1. A composition comprising
a) a stable aqueous dispersion of polymer particles having one or more structural units of i) a polyurea macromer; and ii) an acrylate, a methacrylate, a vinyl ester, or a styrene monomer, or a combination thereof; andor
b) an aqueous mixture of i) polyurea macromer particles and ii) acrylate, methacrylate, vinyl ester, or styrenic polymer particles, or a combination thereof, wherein the polyurea macromer is characterized by the following formula I:
wherein n is 1 to 20;
R1 is 1,6-hexanediyl,
wherein the dotted lines represent the points of attachment;
R2 and R3 are each independently C2-C10 linear or branched alkanediyl groups, benzenediyl groups, benzenedimethanediyl groups, or cyclohexanediyl groups;
X and Y are independently O or NR4, wherein R4 is H or C1-C6-alkyl, with the proviso that at least one of X and Y is NH;
at least one of A1 and A2 comprise carboxylic acid functionalized groups selected from the group consisting of:
\u2014C(O)\u2014(Z)m\u2014R5; \u2014CH2CH(OH)(CH2)pCOOH; and \u2014CR7\u2550CH\u2014C(O)\u2014O\u2014(CH2)pCOOH;
wherein each R5 is independently
with the proviso that when one of A1 and A2 does not contain carboxylic acid functional groups, the non-carboxylic acid functionalized group is selected from the group consisting of:
\u2014CH2CH(OH)R6; \u2014CR7\u2550CH\u2014C(O)\u2014O\u2014(CH2)pR8; \u2014C(O)\u2014Y\u2032\u2014(CH2)pR9; and \u2014CH2\u2014CH2\u2014C(O)\u2014O\u2014R10; wherein
each R6 is independently \u2014CH2-acrylate or \u2014CH2-methacrylate;
each R7 is independently H or CH3;
each R8 is independently an acrylate group or a methacrylate group;
each R9 is independently an acrylate group or a methacrylate group;
each R10 is independently H or \u2014CH2CH\u2550CH2;
each R11 is independently a linear or branched C1-C15 alkyl group;
Y\u2032 is O or NR4;
m is 0 or 1;
Z is a remnant of a nucleophile; and
each p is independently from 2 to 6.
2. The composition of claim 1 wherein X and Y are each NH; n is from 2 to 10; and the polymer particles comprise from 2 to 30 weight percent structural units of the polyurea macromer and from 70 to 98 weight percent of structural units of the acrylate, the methacrylate, or the styrene, or a combination thereof, wherein A1 and A2 are each independently:
\u2014C(O)\u2014(Z)m\u2014R5; \u2014CH2CH(OH)R6; \u2014CR7\u2550CH\u2014C(O)O(CH2)pR8; \u2014C(O)\u2014Y\u2032\u2014(CH2)pR9; or \u2014CH2CH2\u2014C(O)\u2014O\u2014R10.
3. The composition of claim 2 wherein
R1 is
and R2 and R3 are each independently linear or branched C3-C10-alkanediyl groups.
4. The composition of claim 2 wherein
m is 0; and
R2 and R3 are each \u2014CH2CH(CH3)\u2014 groups; and
A1 and A2 are independently \u2014C(O)\u2014R5.
5. The composition of claim 2 wherein
m is 0; and
at least one of A1 and A2 is \u2014CH2\u2014CH(OH)R6, wherein R6 is \u2014CH2-methacrylate
or \u2014(CH2)p\u2014COOH, wherein p is 2 to 6.
6. The composition of claim 2 wherein at least one of A1 and A2 is
\u2014CR7\u2550CH\u2014C(O)\u2014O\u2014(CH2)pR8; wherein R7 is H, R8 is a methacrylate group or COOH.
7. The composition of claim 2 wherein at least one of A1 and A2 is \u2014C(O)\u2014O\u2014(CH2)pR9 wherein R9 is a methacrylate group; or wherein at least one of A1 and A2 is \u2014CH2\u2014CH2\u2014C(O)O\u2014R10.
8. The composition of claim 7 wherein one of A1 and A2 is \u2014C(O)\u2014O\u2014(CH2)pR9 wherein R9 is a methacrylate group; and wherein the other of A1 and A2 is \u2014CH2\u2014CH2\u2014C(O)\u2014O\u2014R10.
9. The composition of claim 1 wherein one or both of A1 and A2 comprise carboxylic acid functionalized groups selected from the group consisting of:
\u2014C(O)\u2014(Z)m\u2014R5; \u2014CH2CH(OH)(CH2)pCOOH; and \u2014CR7\u2550CHC(O)O(CH2)pCOOH;
wherein R5 is
with the proviso that when one of A1 and A2 does not contain carboxylic acid functional groups, the non-carboxylic acid functionalized group is selected from the group consisting of:
\u2014CH2CH(OH)R6; \u2014CR7\u2550CHC(O)O(CH2)pR8; and \u2014C(O)\u2014Y\u2032\u2014(CH2)pR9;
R6 is \u2014CH2-acrylate, or \u2014CH2-methacrylate;
R8 is an acrylate group or a methacrylate group;
R9 is an acrylate group or a methacrylate group; and
wherein X and Y are each NH; and the polymer particles comprise from 2 to 30 weight percent structural units of the polyurea macromer and from 70 to 98 weight percent of structural units of the acrylate, the methacrylate, or the styrene, or a combination thereof.
10. The composition of claim 9 wherein A1 and A2 are each
independently \u2014C(O)(O)m\u2014R5; \u2014CH2CH(OH)(CH2)pCOOH; or \u2014CR7\u2550CHC(O)O(CH2)pCOOH.
11. The composition of claim 10 wherein
m is 0 and n is 2 to 10.
12. The composition of claim 11 wherein
R1 is
wherein the dotted lines represent the points of attachment; and
R2 and R3 are each independently linear or branched C3-C10-alkanediyl groups.
13. The composition of claim 12 wherein
A1 and A2 are each \u2014C(O)\u2014R5;
each R5 is independently:
14. The composition of claim 9 wherein
m is 0; and
one of A1 and A2 is \u2014CH2\u2014CH(OH)R6, wherein R6 is \u2014CH2-methacrylate or \u2014(CH2)p\u2014COOH.
15. The composition of claim 9 wherein
at least one of A1 and A2 is \u2014CH\u2550CHC(O)O(CH2)pCOOH.
16. The composition of claim 9 wherein
one of A1 and A2 is \u2014C(O)O(CH2)pR9, wherein R9 is a methacrylate group; and the other
of A1 and A2 is \u2014C(O)CH2CH2COOH.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of manufacturing a micro-chamber plate with a built-in sample, comprising a step S20 of settling a micro-chamber plate 100 for sample injection at a micro-chamber plate receiving part 200 formed with an upper opening;
a step S30 of disposing a cover 310 for micro-chamber plate receiving part so as to cover the upper opening of the micro-chamber plate receiving part 200, the cover 310 for micro-chamber plate receiving part comprising a provisional storing part 312 and an auxiliary covering part 314 connected with the provisional storing part 312 and formed with a through-hole 314-1 for auxiliary covering part; and
a step S40 of manufacturing a micro-chamber plate 100A with a built-in sample by putting the micro-chamber plate receiving part 200, on which the cover 310 for micro-chamber plate receiving part is disposed, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part 312 into the micro-chamber plate 100 for sample injection through a vessel communication part which is formed at the provisional storing part 312 so as to be communicated with the micro-chamber plate receiving part 200.
2. The method according to claim 1, wherein the step S40 of manufacturing the micro-chamber plate 100A with the built-in sample comprises:
a vacuum and centrifugal force applying step S41 of applying a vacuum into the centrifugal separator and generating first centrifugal force while the vacuum is applied into the centrifugal separator; and
a vacuum releasing and centrifugal force applying step S42 of injecting the sample solution into the micro-chamber plate 100 for sample injection by releasing the vacuum in the centrifugal separation while second centrifugal force larger than the first centrifugal force is generated by the centrifugal separator.
3. The method according to claim 2, wherein the first centrifugal force is a centrifugal force which can suppress bumping of the sample solution, while the vacuum is applied into the centrifugal separator.
4. The method according to claim 3, wherein the vessel communication part is a cutting line 312-1 which is formed at the provisional storing part 312 so as to be opened by external force, and
the second centrifugal force is a centrifugal force which can open the cutting line 312-1.
5. A method of manufacturing an analytic micro-chamber plate using the micro-chamber plate with the built-in sample manufactured by the method according to claim 1, comprising:
a step S50 of manufacturing the analytic micro-chamber plate by taking out the micro-chamber plate 100A with the built-in sample from the centrifugal separator and then sealing a separation membrane 130 of the micro-chamber plate 100A with the built-in sample.
6. A method of manufacturing a micro-chamber plate with a built-in sample, comprising:
a step S200 of forming a sample solution storing space between a cover 1310 for micro-chamber plate receiving part and an upper surface of a micro-chamber plate 100 for sample injection by closely contacting a lower end of a cover 1310 for micro-chamber plate receiving part to the upper surface of the micro-chamber plate 100 for sample injection, the cover 1310 for micro-chamber plate receiving part comprising a provisional storing part 1312 and an auxiliary covering part 1314 connected with the provisional storing part 1312 and formed with a through-hole 1314-1 for auxiliary covering part; and
a step S300 of manufacturing a micro-chamber plate with a built-in sample by putting the micro-chamber plate 100 for sample injection and the cover 1310 for micro-chamber plate receiving part, which are closely contacted with each other so as to form the sample solution space therebetween, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part 1312 into the micro-chamber plate 100 for sample injection through a vessel communication part which is formed at the provisional storing part 1312 so as to be communicated with the sample solution storing space.
7. The method according to claim 6, wherein the step S300 of manufacturing the micro-chamber plate with the built-in sample comprises:
a vacuum and centrifugal force applying step of applying a vacuum into the centrifugal separator and generating first centrifugal force while the vacuum is applied into the centrifugal separator; and
a vacuum releasing and centrifugal force applying step of injecting the sample solution into the micro-chamber plate 100 for sample injection by releasing the vacuum in the centrifugal separation while second centrifugal force larger than the first centrifugal force is generated by the centrifugal separator.
8. The method according to claim 7, wherein the first centrifugal force is a centrifugal force which can suppress bumping of the sample solution, while the vacuum is applied into the centrifugal separator.
9. The method according to claim 8, wherein the vessel communication part is a cutting line 1312-1 which is formed at the provisional storing part 1312 so as to be opened by external force, and
the second centrifugal force is a centrifugal force which can open the cutting line 312-1.
10. A method of manufacturing an analytic micro-chamber plate using the micro-chamber plate with the built-in sample manufactured by the method according to claim 6, comprising:
a step S50 of manufacturing the analytic micro-chamber plate by taking out the micro-chamber plate 100A with the built-in sample from the centrifugal separator and then sealing a separation membrane 130 of the micro-chamber plate 100A with the built-in sample.
11. An analytic micro-chamber plate in which a body sealing part 120 is formed at a lower surface thereof, a sealed separation membrane is formed at an upper surface thereof, and the unit number of chamber holes 112, which a sample solution including nucleic acid and a special component 140 for analyzing the nucleic acid are built in, is formed, wherein the body sealing part 120 is formed of a material that reflects light, and the sealed separation membrane is a separation membrane 130 which is formed of a porous material and coated and sealed with polymer oil so that a surface of the separation membrane 130 has an increased optical transparency.
12. An apparatus set for manufacturing a micro-chamber plate with a built-in sample, comprising:
a micro-chamber plate receiving part 200 which is formed with an upper opening; and
a cover 310 for a micro-chamber plate receiving part, which comprises a provisional storing part 312 and an auxiliary covering part 314 connected with the provisional storing part 312 and formed with a through-hole 314-1 for auxiliary covering part, and which covers an upper opening of the micro-chamber plate receiving part 200, the provisional storing part 312 being formed with a vessel communication part which can be opened and closed and is communicated with the micro-chamber plate receiving part 200 when being opened.
13. The apparatus set according to claim 12, further comprising a cover 320 for provisional storing part which exposes the through-hole 314-1 for auxiliary covering part to an outside and partially closes the provisional storing part 312.
14. The apparatus set according to claim 13, wherein a lower surface of the provisional storing part 312 is inserted into the micro-chamber plate receiving part 200, and
an upper end of the provisional storing part 312 and the auxiliary covering part 314 are disposed at an upper end of the micro-chamber plate receiving part 200.
15. The apparatus set according to claim 12, further comprising a cover 320 for provisional storing part which covers an upper end of the through-hole 314-1 for auxiliary covering part and an upper end of the provisional storing part 312, wherein the cover 320 for provisional storing part is a membrane filter which allows penetration of gas and prevents penetration of the sample solution.
16. The apparatus set according to claim 12, wherein the vessel communication part is a cutting line 312-1 which is opened by external force.
17. An apparatus set for manufacturing a micro-chamber plate with a built-in sample, comprising:
a cover 1310 for micro-chamber plate receiving part, which comprises a provisional storing part 1312 and an auxiliary covering part 1314 connected with the provisional storing part 1312 and formed with a through-hole 1314-1 for auxiliary covering part, and of which a lower end is closely contacted with an upper surface of a micro-chamber plate 100 for sample injection by coupling means so that a sample solution storing space S is formed between the cover 1310 for micro-chamber plate receiving part and the upper surface of the micro-chamber plate 100 for sample injection,
wherein the provisional storing part 1312 is formed with a vessel communication part which can be opened and closed and is communicated with the sample solution storing space when being opened.
18. The apparatus set according to claim 17, wherein the coupling means comprises:
a micro-chamber plate receiving part 1200 in which the micro-chamber plate 100 for sample injection is settled; and
a coupling case 1400 of which an upper surface is formed with the through-hole 1314-1 for auxiliary covering part and a through-hole 1424 for case communicating the provisional storing part 1312 to an outside, and which compresses an upper end of the cover 1310 for micro-chamber plate receiving part so as to be coupled to the micro-chamber plate receiving part 1200.
19. The apparatus set according to claim 17, wherein the vessel communication part is a cutting line 1312-1 which is opened by external force.
20. The apparatus set according to claim 17, further comprising a case cover 1500 which is attached to the coupling case 1400 so as to cover the through-hole 1424 for case, such that the through-hole 1314-1 is exposed to an outside and the provisional storing part is closed partially.
21. The apparatus set according to claim 17, wherein the case cover 1500 is attached to the coupling case 1400 so as to cover the through-hole 1424, and
the case cover 1500 is a membrane filter which allows penetration of gas and prevents penetration of the sample solution.