1. An isolated polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3;
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
2. An isolated polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4;
b) a proregion comprising the sequence of SEQ ID No. 5;
c) a mature peptide comprising the sequence SEQ ID No. 6;
d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or
e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6;
wherein said signal peptide causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said proregion inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5;
wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity;
wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and
wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
3. An isolated polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6 and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
4. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide fragment comprising at least 15 consecutive amino acids.
5. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide comprising the sequence of SEQ ID No. 3.
6. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3.
7. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of the polypeptide of SEQ ID No. 3.
8. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a signal peptide comprising the sequence of SEQ ID No. 4.
9. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a proregion comprising the sequence of SEQ ID No. 5.
10. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a mature peptide comprising the sequence of SEQ ID No. 6.
11. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6.
12. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of the signal peptide comprising the sequence of SEQ ID No. 4.
13. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of a proregion comprising the sequence of SEQ ID No. 5.
14. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of a mature peptide comprising the sequence of SEQ ID No. 6.
15. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising the sequence of SEQ ID No. 6.
16. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6.
17. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6.
18. The isolated polynucleotide according to claim 3, wherein said polynucleotide encodes a polypeptide comprising at least 15 consecutive amino acids.
19. The isolated polynucleotide according to claim 2, wherein said polynucleotide encodes a polypeptide comprising at least 15 consecutive amino acids.
20. The isolated polynucleotide according to claim 11, wherein said polynucleotide encodes a polypeptide having an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4.
21. The isolated polynucleotide according to claim 1, wherein said polynucleotide encodes a polypeptide having an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 5.
22. The isolated polynucleotide according to claim 11, wherein said polynucleotide encodes a polypeptide having an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 6.
23. A vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3;
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
24. A vector comprising a polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4;
b) a proregion comprising the sequence of SEQ ID No. 5;
c) a mature peptide comprising the sequence SEQ ID No. 6;
d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or
e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6;
wherein said signal peptide causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said proregion inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5;
wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity;
wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and
wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
25. A vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6 and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6;
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
26. The vector according to claim 23, further comprising elements ensuring the expression of said polynucleotide in a host cell.
27. The vector according to claim 24, further comprising elements ensuring the expression of said polynucleotide in a host cell.
28. The vector according to claim 25, further comprising elements ensuring the expression of said polynucleotide in a host cell.
29. A host cell transformed with a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3;
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
30. A host cell transformed with a vector comprising a polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4;
b) a proregion comprising the sequence of SEQ ID No. 5;
c) a mature peptide comprising the sequence SEQ ID No. 6;
d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or
e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6;
wherein said signal peptide causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said proregion inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5;
wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity;
wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and
wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
31. A host cell comprising a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6; and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
32. A method of producing a polypeptide comprising culturing a host cell transformed with the vector comprising the polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 3;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 3 and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of SEQ ID No. 3;
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3, antimicrobial activity, and cytotoxic activity.
33. A method of producing a polypeptide comprising culturing a host cell transformed with a vector comprising the polynucleotide encoding a polypeptide comprising:
a) a signal peptide comprising the sequence of SEQ ID No. 4;
b) a proregion comprising the sequence of SEQ ID No. 5;
c) a mature peptide comprising the sequence SEQ ID No. 6;
d) a polypeptide comprising an amino acid sequence at least 90% identical over the full length to the amino acid sequence of SEQ ID No. 4, SEQ ID No. 5, or SEQ ID No. 6; or
e) a fragment comprising at least 10 consecutive amino acids of SEQ ID NO: 4, SEQ ID NO. 5, or SEQ ID NO. 6;
wherein said signal peptide causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said proregion inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5;
wherein said mature peptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity;
wherein said fragment of SEQ ID No. 4 causes intra- or extracellular secretion of a polypeptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 4;
wherein said fragment of SEQ ID No. 5 inactivates the precursor form of the defensin molecule andor provides a support for the acquisition of the active conformation of the mature peptide andor is recognized by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO: 5; and
wherein said fragment of SEQ ID No. 6 has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID No. 3 or SEQ ID NO 6, antimicrobial activity, and cytotoxic activity.
34. A method of producing a polypeptide comprising culturing a host cell transformed with a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of:
a) a polypeptide comprising the sequence of SEQ ID No. 6;
b) a polypeptide comprising an amino acid sequence at least 80% identical over the full length to the amino acid sequence of SEQ ID No. 6 and
c) a polypeptide comprising a fragment of at least 10 consecutive amino acids of the sequence of SEQ ID No. 6
wherein said isolated polypeptide has at least one biological activity selected from the group consisting of recognition by an antibody specific for the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 6, antimicrobial activity, and cytotoxic activity.
35. The transformed host cell according to claim 29, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
36. The transformed host cell according to claim 30, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
37. The transformed host cell according to claim 31, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
38. The method according to claim 32, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
39. The method according to claim 33, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
40. The method according to claim 34, wherein said vector further comprises elements ensuring the expression of said polynucleotide in said host cell.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A magnetic resonance imaging permanent magnet system comprising:
a generally C-shaped yoke (1) formed from a single piece of cast steel;
said yoke (1) having a vertical post (24), said vertical post having two ends, said yoke further comprising diagonal sections (20, 22) extending from each end of said vertical post, said diagonal sections having angled inside and outside faces, said inside faces defining an angle of approximately 45 degrees in relation to said vertical post, and said yoke further comprising substantially horizontal arms (10, 12, 16; and 10, 14, 18) extending from each of said diagonal sections;
a pair of poles (2) supported on said horizontal arms (10), each of said poles (2) being made substantially of permanently magnetized NdFeB and having respective pole faces which are opposed to one another and in a spaced relation to define an imaging volume between said pole faces;
said vertical post having a portion of reduced width at its medial extent, with said vertical post (24) being wider at an intersection thereof with said diagonal sections (20,22);
the ends of said horizontal arms (12, 14) adjacent said imaging volume being beveled inward on the opposite side from the imaging volume;
a substantially flat permanently magnetized NdFeB plate (3) mounted on the surface of the inside face of each of said diagonal sections (20, 22) in face to face relationship with said inside face toward said imaging volume, said inside face and said plate (3) defining an angle of approximately 45 degrees in relation to said horizontal post, said plates (3) being poled to oppose leakage flux between the nearest pole (2) and the post, and to maintain the flux both in the imaging volume and in the vertical post;
a pair of composite pole plates (4), one pole plate mounted on each of said pole faces on either side of the imaging volume, each of said pole plates (4) comprising a tape wound laminated thin sheet of high resistivity silicon steel with an insulating adhesive between each layer of the tape, and wherein said laminated sheet is segmented into a plurality of wedge-shaped elements, with said wedge-shaped elements being formed into a circular disk shape with an insulating adhesive between the respective wedge-shaped elements; and
a pole ring (5) mounted on each of said pole plates (4).
2. A magnet system as in claim 1 wherein said imaging volume has a vertical extent of at least 0.47 m, with the diameter of said poles (2) being between 1.0 m and 1.2 m, and the thickness of said pole plates (4) being between 45 mm and 60 mm.
3. A magnet system as in claim 2 wherein the vertical post (24) has a vertical extent between 1.8 m and 1.9 m, with the width of said diagonal sections being between 1.2 m and 1.4 m, and with the depth of said vertical post (24) being between 1.7 m and 1.9 m.