1. A method of altering read, transmit or detection characteristics of a radio frequency identification (RFID) device, said method comprising the steps of:
obtaining an RFID device, the RFID device comprising a substrate, a transponder chip and an antenna coupled to the chip; and
reducing a read, transmit or detection range of the RFID device via reducing an amount of the antenna that is coupled to the chip;
wherein said step of reducing the amount of the antenna comprises altering a substantial portion of the substrate andor the antenna;
wherein the RFID device is initially operable within an original range; and
wherein said step of reducing the read, transmit or detection range comprises leaving a remainder device sufficient for responding to a signal solely at a range shorter than the original range.
2. The method according to claim 1, wherein said step of leaving a remainder comprises leaving a remainder device sufficient for responding to a signal solely at a range less than approximately 15 mm.
3. The method according to claim 1, wherein said step of altering a substantial portion of the substrate andor the antenna comprises a step taken from a group of steps consisting of: exposing the device to a suitable solvent; laundering an object to which the device is coupled; and applying a physical force to at least part of the device.
4. The method according to claim 1, wherein the substrate andor the antenna includes soluble material.
5. The method according to claim 4, wherein the soluble material includes a water-soluble material.
6. The method according to claim 4, wherein the soluble material includes a material that is soluble in a mixture of water and a laundry detergent.
7. The method according to claim 4, wherein the soluble material includes a material that is soluble when a solvent is in a predetermined temperature range.
8. The method according to claim 4, wherein the soluble material includes a material that is soluble in a dry cleaning fluid.
9. The method according to claim 4, wherein the soluble material includes a material that is soluble in carbon tetrachloride.
10. The method according to claim 1, wherein the antenna includes a soluble conductive material.
11. The method according to claim 10, wherein the soluble conductive material includes a soluble conductive ink.
12. The method according to claim 1, wherein said step of altering a substantial portion of the substrate andor the antenna changes an operating frequency of the device.
13. A radio frequency identification (RFID) device comprising:
a substrate, a transponder chip and an antenna coupled to the chip; and
an arrangement for reducing a read, transmit or detection range of the RFID device via reducing an amount of the antenna that is coupled to the chip;
wherein said arrangement for reducing a read or detection range comprises an arrangement for altering a substantial portion of the substrate andor the antenna;
wherein said RFID device is initially operable within an original range; and
wherein said arrangement for reducing the read, transmit or detection range is adapted to leave a remainder device sufficient for responding to a signal solely at a range shorter than the original range.
14. The RFID device according to claim 13, wherein said arrangement for reducing the read, transmit or detection range is adapted to leave a remainder device sufficient for responding to a signal solely at a range less than approximately 15 mm.
15. The RFID device according to claim 13, wherein said arrangement for altering a substantial portion of the substrate andor the antenna comprises an arrangement taken from a group of arrangements consisting of: an arrangement for facilitating exposing the device to a suitable solvent; an arrangement for facilitating laundering an object to which the device is coupled; and an arrangement for facilitating applying a physical force to at least part of the device.
16. The RFID device according to claim 13, wherein the substrate andor the antenna includes soluble material.
17. The RFID device according to claim 16, wherein the soluble material includes a water-soluble material.
18. The RFID device according to claim 16, wherein the soluble material includes a material that is soluble in a mixture of water and a laundry detergent.
19. The RFID device according to claim 16, wherein the soluble material includes a material that is soluble when a solvent is in a predetermined temperature range.
20. The RFID device according to claim 16, wherein the soluble material includes a material that is soluble in a dry cleaning fluid.
21. The RFID device according to claim 16, wherein the soluble material includes a material that is soluble in carbon tetrachloride.
22. The RFID device according to claim 13, wherein the antenna includes a soluble conductive material.
23. The RFID device according to claim 22, wherein the soluble conductive material includes a soluble conductive ink.
24. The RFID device according to claim 13, wherein said arrangement for altering a substantial portion of the substrate andor the antenna changes an operating frequency of the device.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A nucleic acid adaptor, comprising:
a double stranded nucleic acid element that comprises a plurality of deoxyinosine species positionally located in a spaced relationship from each other on a first strand and base pair to an A, T, or G nucleotide species on a second strand, wherein a first end of the double stranded nucleic acid element is constructed and arranged to preferentially ligate to each end of a double stranded target nucleic acid molecule.
2. The nucleic acid adaptor of claim 1, wherein:
the first strand comprises a 3\u2032 single base overhang and a second strand comprises a 5\u2032 phosphate group that preferentially ligates the first end of the double stranded nucleic acid element to each end of the double stranded target nucleic acid molecule.
3. The nucleic acid adaptor of claim 1, wherein:
the first strand comprises a detectable moiety at the 5\u2032 terminus.
4. The nucleic acid adaptor of claim 1, wherein:
the second strand comprises a detectable moiety at the 3\u2032 terminus.
5. The nucleic acid adaptor of claim 1, wherein:
the deoxyinosine species are spaced 4 or more sequence positions away from each other.
6. The nucleic acid adaptor of claim 1, wherein:
the deoxyinosine species are spaced no closer than 6 sequence positions from an end of the double stranded nucleic acid element arranged to ligate to a double stranded target nucleic acid molecule.
7. The nucleic acid adaptor of claim 1, wherein:
the first and the second strands comprise a plurality of phophorothioates at each end.
8. The nucleic acid adaptor of claim 7, wherein:
one or more of the phophorothioates at each end of the first strand are positionally located in a complementary relationship to one or more of the phosphorothioates at each end of the second strand.
9. The nucleic acid adaptor of claim 8, wherein:
the first and the second strands comprise at least 4 phosphorothioates at each end, wherein the phosphorothioates are interspaced by a natural nucleotide species.
10. A kit comprising the nucleic acid adaptor of claim 1.
11. A method for preparing a nucleic acid library, comprising the steps of:
ligating a fully complementary double stranded nucleic acid adaptor to each end of a double stranded nucleic acid target molecule to produce a ligated double stranded adaptor-target-adaptor molecule, wherein the fully complementary double stranded nucleic acid adaptor comprises a plurality of deoxyinosine species positionally located in a spaced relationship from each other on a first adaptor strand that base pair to an A, T, or G nucleotide species on a second adaptor strand;
denaturing the double stranded adaptor-target-adaptor molecule to produce two single stranded adaptor-target-adaptor molecules, wherein the single stranded adaptor-target-adaptor molecules comprise a first region from the first adaptor strand at a first end, a middle region from the nucleic acid target molecule, and a second region from the second adaptor strand at a second end;
annealing a first primer to the second region of a first strand of the single stranded adaptor-target-adaptor molecules;
extending the first primer to produce a second strand complementary to the first strand, wherein the second strand comprises a third region at the second end comprising C nucleotide species base paired to the deoxyinosine species in the first region of the first strand;
annealing a second primer to the third region of second strand; and
extending the second primer to produce a third strand complementary to the second strand, wherein the second and third strands comprise semi-complementary ends resistant to hybridization to each other.
12. The method of claim 11, comprising:
the second strand comprises sequence composition from the first primer at a first end, a middle region from the nucleic acid target molecule, and the third region at a second end.
13. The method of claim 11, comprising:
the third strand comprises second region at a first end, a middle region from the nucleic acid target molecule, and sequence composition from the second primer at a second end.
14. The method of claim 11, comprising:
the second strand and the third strand are each sequencable products.
15. The method of claim 11, further comprising the step of:
clonally amplifying the second strand to produce a population of substantially identical copies; and
sequencing the population of substantially identical copies to produce a sequence composition of the second strand.
16. The method of claim 11, further comprising the step of:
clonally amplifying the third strand to produce a population of substantially identical copies; and
sequencing the population of substantially identical copies to produce a sequence composition of the third strand.
17. The nucleic acid adaptor of claim 11, wherein:
the first adaptor strand comprises a 3\u2032 single base overhang and the second adaptor strand comprises a 5\u2032 phosphate group that preferentially ligates a first end of the the fully complementary double stranded nucleic acid adaptor to each end of the double stranded target nucleic acid molecule.
18. The nucleic acid adaptor of claim 11, wherein:
the deoxyinosine species are spaced 4 or more sequence positions away from each other.
19. The nucleic acid adaptor of claim 11, wherein:
the deoxyinosine species are spaced no closer than 6 sequence positions from an end of the fully complementary double stranded nucleic acid adaptor arranged to ligate to the double stranded target nucleic acid molecule