What is claimed is:
1. A single-stranded nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, said electron donor moiety and said electron acceptor moiety being covalently attached to said nucleic acid.
2. A single-stranded nucleic acid according to claim 1 wherein said covalent attachment is to the ribose-phosphate backbone of said nucleic acid.
3. A single stranded nucleic acid according to claim 1, wherein said single stranded nucleic acid is capable of hybridizing to a complementary target sequence in a second single stranded nucleic acid to form a hybridization complex.
4. A single stranded nucleic acid according to claim 3, wherein said hybridization complex is capable of transferring at least one electron between said electron donor moiety and said electron acceptor moiety.
5. A composition comprising a first single stranded nucleic acid containing at least one electron donor moiety and a second single stranded nucleic acid containing at least one electron acceptor moiety, wherein said electron donor moiety and electron acceptor moiety are covalently linked to the ribose-phosphate backbone of said first and second single stranded nucleic acids.
6. A composition according to claim 5 wherein said first single stranded nucleic acid is capable of hybridizing to said second single stranded nucleic acid to form a double stranded nucleic acid.
7. A double stranded nucleic acid composition according to claim 6 wherein said first single stranded nucleic acid is hybridized to said second single stranded nucleic acid.
8. A composition according to claim 7 wherein said composition is capable of transferring at least one electron between said electron donor moiety and said electron acceptor moiety.
9. A composition according to claim 5 wherein said first and second single stranded nucleic acids are capable of hybridizing to a target sequence stranded nucleic acid, comprising at least a first target domain and a second target domain, wherein said first nucleic acid is capable of hybridizing to said first target domain and said second nucleic acid is capable of hybridizing to said second target domain to form a hybridization complex.
10. A composition according to claim 9 wherein said first target domain and said second target domain are adjacent to one another.
11. A composition according to claim 10 wherein said first nucleic acid and said second nucleic acid in said hybridization complex are ligated together.
12. A composition according to claim 10 wherein said hybridization complex is capable of transferring at least one electron between said electron donor moiety and said electron acceptor moiety.
13. A composition according to claim 10 wherein said target sequence further comprises an intervening target domain between said first and said second target domain.
14. A composition according to claim 13 further comprising an intervening single stranded nucleic acid sequence capable of hybridizing to said intervening target domain to form a hybridization complex.
15. A composition according to claim 14 wherein said hybridization complex is capable of transferring at least one electron between said electron donor moiety and said electron acceptor moiety.
16. A method for making a single stranded nucleic acid containing an electron transfer moiety at the 5 terminus, comprising
a) incorporating a modified nucleotide into a growing nucleic acid at the 5 position to form a modified single stranded nucleic acid;
b) hybridizing said modified single stranded nucleic acid with a complementary single stranded nucleic acid to form a double stranded nucleic acid;
c) reacting said double stranded nucleic acid with an electron transfer moiety such that said moiety is covalently attached to said modified single stranded nucleic acid; and
d) separating said complementary single stranded nucleic acid from said modified single stranded nucleic acid containing said electron transfer moiety.
17. A method for making a single stranded nucleic acid containing an electron transfer moiety covalently attached to an internal nucleotide, comprising
a) incorporating a modified nucleotide dimer into a growing nucleic acid to form a modified single stranded nucleic acid;
b) hybridizing said modified single stranded nucleic acid with a complementary single stranded nucleic acid to form a double stranded nucleic acid;
c) reacting said double stranded nucleic acid with an electron transfer moiety such that said moiety is covalently attached to said modified single stranded nucleic acid; and
d) separating said complementary single stranded nucleic acid from the modified single stranded nucleic acid containing said electron transfer moiety.
18. A method for making a single stranded nucleic acid containing an electron transfer moiety covalently attached to the 3 terminal nucleotide, comprising
a) incorporating a modified nucleotide via enzymatic addition or replacement into a nucleic acid;
b) hybridizing said modified single stranded nucleic acid with a complementary single stranded nucleic acid to form a double stranded nucleic acid;
c) reacting said double stranded nucleic acid with an electron transfer moiety such that said moiety is covalently attached through said phosphoramide bond of said modified single stranded nucleic acid; and
d) separating said complementary single stranded nucleic acid from the modified single stranded nucleic acid containing said electron transfer moiety.
19. A method of detecting a target sequence in a nucleic acid sample comprising
a) hybridizing a single stranded nucleic acid containing at least one covalently attached electron donor moiety and at least one covalently attached electron acceptor moiety to said target sequence to form a hybridization complex;
b) determining the electron transfer rate between said electron donor moiety and said electron acceptor moiety in the hybridization complex; and
c) comparing said electron transfer rate with the electron transfer rate in the absence of the target sequence as an indicator of the presence or absence of said target sequence.
20. A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, wherein said method comprises:
a) hybridizing a first nucleic acid containing at least one electron donor moiety to said first target domain;
b) hybridizing a second nucleic acid containing at least one electron acceptor moiety to said second target domain;
c) determining the electron transfer rate between said electron donor moiety and said electron acceptor moiety while said first and second nucleic acids are hybridized to said first and second target domains; and
d) comparing said electron transfer rate with the electron transfer rate in the absence of the target sequence as an indicator of the presence or absence of said target sequence in said nucleic acid sample.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An image processing device comprising:
a first control unit to control a normal operating mode during which image processing is performed;
a second control unit to control an energy-saving mode during which power consumption is lower than during the normal operating mode;
an interrupt request receiver connected to an external device to receive an interrupt request from the external device;
interrupt factor distributors to distribute the interrupt request received by the interrupt request receiver based on whether or not the interrupt request includes a predetermined interrupt factor;
interrupt request notification units to notify the second control unit as well as the first control unit of the interrupt request in accordance with distribution results from the interrupt factor distributors;
job adjustors to control transfer of a job between the first control unit and the second control unit based on notification from the interrupt request notification units;
a detector to detect a power recovery factor;
a measuring unit to measure a time for the energy-saving mode;
a power supply unit to supply power to the first control unit when the time measured by the measuring unit reaches a predetermined period of time; and
a status notification unit to notify the detector of a status of the first control unit to which power is supplied from the power supply unit,
wherein an operating mode of the image processing device is changed from the energy-saving mode to the normal operating mode based on the power recovery factor detected by the detector.
2. The image processing device according to claim 1, wherein the predetermined interrupt factor is stored in a register.
3. The image processing device according to claim 2, wherein each of the first control unit and the second control unit comprises the register.
4. The image processing device according to claim 1, further comprising:
a request signal control unit to control whether or not the operating mode of the image processing device is changed to the normal operating mode based on the power recovery factor detected by the detector; and
a request signal generator to generate a power-on request signal to change the operating mode of the image processing device to the normal operating mode using the request signal control unit.
5. The image processing device according to claim 4, wherein the request signal control unit comprises a register to prevent the operating mode of the image processing device from being changed to the normal operating mode from the energy-saving mode while the power supply unit supplies power to the first control unit.
6. An image processing method for an image processing device comprising a first control unit to control a normal operating mode during which image processing is performed and a second control unit to control an energy-saving mode during which power consumption is lower than during the normal operating mode, the image processing method comprising:
receiving an interrupt request from an external device connected to the image processing device;
distributing the interrupt request based on whether or not the interrupt request includes a predetermined interrupt factor;
notifying the second control unit as well as the first control unit of the interrupt request in accordance with distribution results obtained by the distributing the interrupt request;
controlling transfer of a job between the first control unit and the second control unit based on notification from the notifying;
detecting a power recovery factor;
measuring a time for the energy-saving mode;
supplying power to the first control unit when the time measured by the measuring reaches a predetermined period of time; and
notifying a status of the first control unit to which power is supplied,
wherein an operating mode of the image processing device is changed from the energy-saving mode to the normal operating mode based on the power recovery factor.
7. The image processing method according to claim 6, wherein the predetermined interrupt factor is stored in a register.
8. The image processing method according to claim 7, wherein each of the first control unit and the second control unit comprises the register.
9. The image processing method according to claim 6, further comprising:
controlling whether or not the operating mode of the image processing device is changed to the normal operating mode based on the power recovery factor; and
generating a power-on request signal to change the operating mode of the image processing device to the normal operating mode.
10. The image processing device according to claim 9, wherein the controlling comprises a register to prevent the operating mode of the image processing device from being changed to the normal operating mode from the energy-saving mode while power is supplied to the first control unit.
11. A recording medium storing an image processing program comprising modules that causes an image processing device comprising a first control unit to control a normal operating mode during which image processing is performed and a second control unit to control an energy-saving mode during which power consumption is lower than during the normal operating mode to execute an image processing method, the image processing method comprising:
receiving an interrupt request from an external device connected to the image processing device;
distributing the interrupt request based on whether or not the interrupt request includes a predetermined interrupt factor;
notifying the second control unit as well as the first control unit of the interrupt request in accordance with distribution results obtained by the distributing the interrupt request;
controlling transfer of a job between the first control unit and the second control unit based on notification from the notifying,
detecting a power recovery factor;
measuring a time for the energy-saving mode;
supplying power to the first control unit when the time measured by the measuring reaches a predetermined period of time; and
notifying a status of the first control unit to which power is supplied, and
an operating mode of the image processing device is changed from the energy-saving mode to the normal operating mode based on the power recovery factor.
12. The recording medium according to claim 11, wherein the predetermined interrupt factor is stored in a register.
13. The recording medium according to claim 12, wherein each of the first control unit and the second control unit comprises the register.
14. The recording medium according to claim 11, wherein the image processing method further comprising:
controlling whether or not the operating mode of the image processing device is changed to the normal operating mode based on the power recovery factor; and
generating a power-on request signal to change the operating mode of the image processing device to the normal operating mode.
15. The recording medium according to claim 14, wherein the controlling comprises a register to prevent the operating mode of the image processing device from being changed to the normal operating mode from the energy-saving mode while power is supplied to the first control unit.