1. An isolated polynucleotide molecule having gene regulatory activity and comprising at least 95 contiguous bases of a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1-4.
2. An isolated polynucleotide molecule having gene regulatory activity and comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1-5.
3. The isolated polynucleotide molecule according to claim 2, wherein said isolated polynucleotide molecule comprises a polynucleotide sequence which exhibits a substantial percent sequence identity of about 98% identity with the polynucleotide sequence of SEQ ID NO: 1-5.
4. A DNA construct comprising an isolated polynucleotide molecule having gene regulatory activity and comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1-5, wherein said isolated polynucleotide molecule is operably linked to a transcribable polynucleotide molecule.
5. The DNA construct of claim 4, wherein said transcribable polynucleotide molecule is a marker gene.
6. The DNA construct of claim 4, wherein said transcribable polynucleotide molecule is a gene of agronomic interest.
7. The DNA construct of claim 6, wherein said gene of agronomic interest is a herbicide tolerance gene selected from the group consisting of genes that encode for phosphinothricin acetyltransferase, glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase, hydroxyphenyl pyruvate dehydrogenase, dalapon dehalogenase, bromoxynil resistant nitrilase, anthranilate synthase, glyphosate oxidoreductase and glyphosate-N-acetyl transferase.
8. A transgenic plant stably transformed with the DNA construct of claim 4.
9. The transgenic plant of claim 8, wherein said plant is a monocotyledonous selected from the group consisting of wheat, maize, rye, rice, oat, barley, turfgrass, sorghum, millet and sugarcane.
10. The transgenic plant of claim 8, wherein said plant is a dicotyledonous plant selected from the group consisting of tobacco, tomato, potato, soybean, cotton, canola, sunflower and alfalfa.
11. A seed of said transgenic plant of claim 9.
12. A seed of said transgenic plant of claim 10.
13. A method of inhibiting weed growth in a field of transgenic glyphosate tolerant crop plants comprising:
i) planting the transgenic plants transformed with an expression cassette comprising (a) an isolated polynucleotide molecule having gene regulatory activity and comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1-5 and operably linked to a DNA molecule encoding a glyphosate tolerance gene and
ii) applying glyphosate to the field at an application rate that inhibits the growth of weeds, wherein the growth and yield of the transgenic crop plant is not substantially affected by the glyphosate application.
14. In the method of claim 13, wherein said glyphosate tolerance gene is selected from the group consisting of a gene encoding for a glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase, a glyphosate oxidoreductase, and a glyphosate-N-acetyltransferase.
15. In the method of claim 13, wherein the transgenic plants are capable of tolerating an application rate up to 256 ouncesacre.
16. In the method of claim 13, wherein the transgenic plants are capable of tolerating an application rate ranging from 8 ouncesacre to 128 ouncesacre.
17. In the method of claim 13, wherein the transgenic plants are capable of tolerating an application rate ranging from 32 ouncesacre to 96 ouncesacre.
18. In the method of claim 13, wherein the application of glyphosate is at least once during the growth of the crop.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1-12. (canceled)
13. A fluids transfer system for medical use comprising a Luer male connector (10) and a Luer female connector (11), of which the said Luer female connector (11) comprises a main body (14) containing an internal channel (15) for the passage of said fluids and a collar (12) surrounding at least a part of the end of the main body, while the said Luer male connector (10) comprises a main body (18) with a longitudinal internal opening (19, 21), characterized in that:
the main body (14) of the female connector contains a first threading (16) on at least a part of the external surface of that portion that is surrounded by the collar (12), located at least in part facing an internal surface of the collar (12),
one of the ends of the main body (18) of the said Luer male connector (10) contains a second threading (20) on at least a part of its internal surface delineating the longitudinal internal opening (19, 21), which engages into and fits in the initial threading (16) to ensure joining between the Luer male and female connectors,
at least the external surface of the Luer male connector (10) end where the second threading (20) is located forms a conical male portion (24) having a conical axis of revolution, while the said interior surface of the collar (12) of the said female connector forms a conical female connecting surface (13) having a conical axis of revolution, so that the external surface of the said male conical part (24) fits against the conical connecting surface of the collar (12) of the Luer female connector (11) to provide a sealed joint when the Luer male and female connectors are fitted together.
14. A system, according to claim 13, characterized in that at least one of the said Luer male (10) and female (11) connectors is made of a single piece and of plastic material.
15. A system according to claim 13, characterized in that the internal channel (15) of the said Luer female connector (11) has a diameter at least equal to the diameter of the internal channel (15) of the male conical portion (24) of the Luer male connector (10) set out in ISO standard 594-2.
16. A system according to claim 13, characterized in that the said Luer male connector (10), andor the Luer female connector (11), contains an internal wall located in its internal longitudinal opening (19, 21), respectively andor in its internal channel (15), with the said internal wall comprising an orifice for the flow of fluids.
17. A system according to claim 16, characterized in that the said Luer male connector (10) with an internal wall containing an orifice in its internal longitudinal opening (19, 21) which orifice has a truncated portion located at the end of the longitudinal internal opening (19, 21) that has a second threading (20), and the end of the main body of the Luer female connector (11) is conical so that with this end being located near the orifice of the said internal wall, fluids are directed through the orifice.
18. A system according to claim 16, characterized in that the said Luer male connector (10) with an internal wall containing an orifice in its longitudinal internal opening (19, 21), which orifice has a portion protruding from the said wall being located at the end of the longitudinal internal opening (19, 21) that comprises said second threading (20), with said protruding portion having an external diameter inferior to the diameter of the internal channel (15) of the main body of the Luer female connector (11) so that it can be received in the internal channel (15) when the said Luer male and female connectors are joined together in order to direct said fluids through the said orifice.
19. A system according to claim 16, characterized in that the internal channel (15) of the said Luer female connector (11) has a diameter at least equal to the diameter of the internal channel (15) of the male conical portion (24) of the Luer male connector (10) set out in ISO standard 594-2.
20. A Luer male connector (10) intended for use in a fluids transfer system for medical use, according to claim 13, characterized in that it comprises:
a main body containing a longitudinal internal opening (19, 21),
one of the ends of the main body of the Luer connector contains a thread on at least a part of its internal surface delineating the longitudinal internal opening (19, 21), which engages into and fits in the threading of a Luer female connector (11) to ensure a secure fit between the Luer male and female connectors,
at least the external surface of the Luer male connector (10) end where the said threading is located forms a conical male portion (24) having a conical axis of revolution, said conical male portion (24) fitting against the conical female connecting surface (13) onto the collar (12) of the Luer female connector (11) when the said Luer male and female connectors are fitted together.
21. A connector, according to claim 20, characterized in that the end of the male connector opposite to the end forming a conical male portion (24) contains gripping elements on its external surface.
22. A connector, according to claim 20, characterized in that it contains a flange ring that forms a stopping point for the collar (12) of the said Luer female connector (11).
23. A Luer female connector (11) intended for use in a fluids transfer system for medical use, according to claim 13, characterized in that the said Luer female connector (11) includes:
a main body containing an internal channel (15) for the passage of fluids and a collar (12) surrounding at least a part of the end of the said main body,
the main body (14) of the female connector contains a first threading (16) on at least a part of the external surface of that portion that is surrounded by the collar (12), located at least in part facing an internal surface of the collar (12)
the internal surface of the collar (12) of the said female connector forms a conical female connecting surface (13) having a conical axis of revolution so that the external surface of the conical male part (24) of a Luer male connector (10) fits against said conical female connecting surface (13) when the Luer male and female connectors are fitted together, said conical male part (24) having a conical axis of revolution.
24. A connector according to claim 23 characterized in that the said part surrounded by the collar (12) includes at least one orifice opening laterally that allows communication of fluids between the internal channel (15) and the area located between the interior surface of the collar (12) and the external surface of the said part in order to limit any loss of fluids during their transfer.
25. A medical device that includes at least one recipient containing fluids for medical use equipped with a fluids transfer system for medical use according to claim 13.
26. A system according to claim 14, characterized in that the internal channel (15) of the said Luer female connector (11) has a diameter at least equal to the diameter of the internal channel (15) of the male conical portion (24) of the Luer male connector (10) set out in ISO standard 594-2.
27. A system according to claim 14, characterized in that the said Luer male connector (10), andor the Luer female connector (11), contains an internal wall located in its internal longitudinal opening (19, 21), respectively andor in its internal channel (15), with the said internal wall comprising an orifice for the flow of fluids.
28. A Luer male connector (10) intended for use in a fluids transfer system for medical use, according to claim 14, characterized in that it comprises:
a main body containing a longitudinal internal opening (19, 21),
one of the ends of the main body of the Luer connector contains a thread on at least a part of its internal surface delineating the longitudinal internal opening (19, 21), which engages into and fits in the threading of a Luer female connector (11) to ensure a secure fit between the Luer male and female connectors,
at least the external surface of the Luer male connector (10) end where the said threading is located forms a conical male portion (24) having a conical axis of revolution, said conical male portion (24) fitting against the conical female connecting surface (13) onto the collar (12) of the Luer female connector (11) when the said Luer male and female connectors are fitted together.
29. A Luer female connector (11) intended for use in a fluids transfer system for medical use, according to claim 14, characterized in that the said Luer female connector (11) includes:
a main body containing an internal channel (15) for the passage of fluids and a collar (12) surrounding at least a part of the end of the said main body,
the main body (14) of the female connector contains a first threading (16) on at least a part of the external surface of that portion that is surrounded by the collar (12), located at least in part facing an internal surface of the collar (12)
the internal surface of the collar (12) of the said female connector forms a conical female connecting surface (13) having a conical axis of revolution so that the external surface of the conical male part (24) of a Luer male connector (10) fits against said conical female connecting surface (13) when the Luer male and female connectors are fitted together, said conical male part (24) having a conical axis of revolution.
30. A medical device that includes at least one recipient containing fluids for medical use equipped with a fluids transfer system for medical use according to claim 14.