What is claimed is:
1. An isolated nucleic acid having a nucleotide sequence selected from the group consisting of:
(a) at least 10 consecutive nucleotides of SEQ ID NO: 1;
(b) at least 12 consecutive nucleotides of SEQ ID NO: 1;
(c) at least 14 consecutive nucleotides of SEQ ID NO: 1;
(d) at least 16 consecutive nucleotides of SEQ ID NO: 1;
(e) at least 18 consecutive nucleotides of SEQ ID NO: 1; and
(f) a sequence complementary to any one of the sequences of (a)-(e).
2. An isolated nucleic acid having a nucleotide sequence selected from the group consisting of:
(a) a sequence encoding a SID-1 protein;
(b) a sequence encoding at least a transmembrane domain of a SID-1 protein;
(c) a sequence encoding at least an extracellular domain of a SID-1 protein; and
(d) a sequence complementary to any one of the sequences of (a)-(c).
3. An isolated nucleic acid encoding a polypeptide having at least 80% amino acid sequence identity with a polypeptide selected from the group consisting of:
(a) a SID-1 protein;
(b) at least a transmembrane domain of a SID-1 protein; and
(c) at least an extracellular domain of a SID-1 protein.
4. An isolated nucleic acid encoding a polypeptide having at least 80% sequence identity with a SID-1 protein and having SID-1 activity in a cell capable of expressing SID-1 activity.
5. An isolated nucleic acid comprising a nucleotide sequence that hybridizes to at least a portion of a nucleic acid of SEQ ID NO: 1 under conditions including a wash step of 1.0SSC at 65 C.
6. The isolated nucleic acid according to claim 5 wherein said nucleic acid encodes a polypeptide having SID-1 activity.
7. A nucleic acid comprising:
(i) a nucleotide sequence encoding a polypeptide having SID-1 activity, wherein said nucleic acid hybridizes to at least a portion of a nucleic acid of SEQ ID NO: 1 under conditions including a wash step of 1.0SSC at 65 C.; and
(ii) a heterologous regulatory region operably joined to said sequence such that said sequence is expressed.
8. A nucleic acid comprising:
(i) a nucleotide sequence encoding a polypeptide having at least 80% amino acid sequence identity with an amino acid sequence of SEQ ID NO:2; and
(ii) a heterologous regulatory region operably joined to said sequence such that said sequence is expressed.
9. The kit for detecting at least a portion of a SID-1 nucleic acid comprising an isolated nucleic acid according to any one of claims 1-8 and a means for detecting said isolated nucleic acid.
10. The kit according to claim 9, wherein said means for detecting said isolated nucleic acid comprises a detectable label bound thereto.
11. A kit according to claim 9, wherein said means for detecting said isolated nucleic acid comprises a labeled secondary nucleic acid which hybridizes to said isolated nucleic acid.
12. A vector comprising an isolated nucleic acid according to any one of claims 1-8.
13. A vector comprising a genetic construct capable of expressing a nucleic acid according to any one of claims 1-6.
14. The vector according to claim 13, wherein said nucleic acid is operably joined to an exogenous regulatory region.
15. The vector according to claim 13, wherein said nucleic acid is operably joined to heterologous coding sequences to form a fusion vector.
16. A vector comprising an isolated nucleic acid according to any one of claims 3-11 operably joined to a reporter gene.
17. A cell transformed with a nucleic acid according to any one of claims 3-11.
18. A cell transformed with a genetic construct capable of expressing a nucleic acid according to any one of claims 3-11.
19. The cell according to claim 18, wherein said nucleic acid is operably joined to heterologous sequences to encode a fusion protein.
20. The cell according to claim 18, wherein said cell is selected from the group consisting of bacterial cells, yeast cells, insect cells, nematode cells, amphibian cells, rodent cells, and human cells.
21. The cell according to in claim 18 wherein said cell is selected from the group consisting of mammalian somatic cells, fetal cells, embryonic stem cells, zygotes, gametes, germ line cells, and transgenic animal cells.
22. A non-human transgenic animal, wherein a genetic construct has introduced a modification into a genome of said animal, or an ancestor thereof, and wherein said modification is selected from the group consisting of insertion of a nucleic acid encoding at least a fragment of a SID-1 protein, inactivation of an endogenous SID-1 protein, and insertion by homologous recombination of a reporter gene operably linked to SID-1 regulatory elements.
23. The animal according to claim 22, wherein said modification is insertion of a nucleic acid encoding a polypeptide selected from the group consisting of a SID-1 protein, at least a transmembrane domain of a SID-1 protein, and at least an extracellular domain of a SID-1 protein.
24. The animal according to claim 23, wherein said animal is selected from the group consisting of rats, mice, hamsters, guinea pigs, rabbit, dogs, cats, goats, sheep, pigs, and non-human primates.
25. A substantially pure protein preparation comprising a polypeptide selected from the group consisting of:
(a) a SID-1 protein;
(b) at least a transmembrane domain of a SID-1 protein; and
(c) at least an extracellular domain of a SID-1 protein.
26. The substantially pure protein preparation according to claim 25, wherein said polypeptide is selected from the group consisting of:
(a) SEQ ID NO: 2;
(b) a sequence comprising a polypeptide encoding residues 314-339, 425-451, 481-502, 509-541, 546-571, 575-599, 601-621, 633-655, 659-681, 692-712, or 742-766 of SEQ ID NO:2; and
(c) a sequence comprising a polypeptide encoding residues 19-314 of SEQ ID NO:2.
27. A substantially pure protein preparation comprising a polypeptide having at least 80% amino acid sequence identity with a polypeptide selected from the group consisting of:
(a) a SID-1 protein;
(b) at least a transmembrane domain of a SID-1 protein; and
(c) at least an extracellular domain of a SID-1 protein.
28. A substantially pure protein preparation comprising a polypeptide having at least 80% amino acid sequence identity with a SID-1 protein and having SID-1 activity in a cell capable of expressing SID-1 activity.
29. A substantially pure antibody preparation comprising an antibody raised against a SID-1 polypeptide.
30. The substantially pure antibody preparation according to claim 29, wherein said antibody is a monoclonal antibody.
31. The substantially pure antibody preparation according to claim 29, wherein said antibody is an antibody fragment selected from the group consisting of an Fab fragment, an F(ab)2 fragment, an Fv fragment, and a single-chain Fv fragment (scFv).
32. A kit for detecting at least an epitope of a SID-1 protein comprising an anti-SID-1 antibody of claims 29 and a means for detecting said antibody.
33. The kit according to claim 33, wherein said means for detecting said anti-SID-1 antibody comprises a labeled secondary antibody which specifically binds to said anti-SID-1 antibody.
34. The kit according to claim 33, wherein said means for detecting said anti-SID antibody comprises a detectable label bound thereto.
35. The kit according to claim 33, wherein the means for detecting the anti-SID antibody comprises a labeled secondary antibody which specifically binds to said anti-SID-1 antibody.
36. A method for reducing the expression of a target gene in a cell, the method comprising:
(a) introducing a nucleic acid vector comprising a SID-1 sequence; and
(b) introducing a double-stranded RNA molecule having a sequence complementary to the target gene,
wherein the SID-1 sequence encodes a polypeptide having SID-1 activity.
37. A method for reducing the expression of a target gene in a population of cells, the method comprising:
(a) introducing a nucleic acid vector comprising a SID-1 sequence, into at least some of the cells; and
(b) introducing a double-stranded RNA molecule having a sequence complementary to the target gene,
wherein the SID-1 sequence encodes a polypeptide having SID-1 activity.
38. A method for reducing the expression of a target gene in an animal, the method comprising:
(a) introducing a nucleic acid vector comprising a SID-1 sequence into the animal; and
(b) introducing a double-stranded RNA molecule having a sequence complementary to the target gene,
wherein the SID-1 sequence encodes a polypeptide having SID-1 activity.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A method comprising the steps of:
providing a voice communication over a conventional telephone network; and
making a payment to a caller for placing a call.
2. A method as recited in claim 1, in which said payment is in the form of cash.
3. A method as recited in claim 1, in which said payment is in the form of a credit.
4. A method as recited in claim 1, in which said payment is in the form of a coupon.
5. An apparatus comprising:
a network;
a first voice terminal; and
a second voice terminal;
a server;
said server being used to connect said first voice terminal to said second voice terminal;
said server including software;
said software being used to issue credits to callers for placing calls.
6. A propagated signal comprising:
an emanation issued from a central computer;
said emanation generated by software running on said central computer;
said emanation being used to furnish a credit to a caller for placing a call.
7. A method comprising the steps of:
detecting a frame of mind of a consumer;
presenting a precisely targeting promotion to said consumer that is triggered by the detection of said frame of mind.
8. A method as recited in claim 7, in which said frame of mind of a consumer is detected by sensing a spoken keyword using a speech recognition engine.
9. A method as recited in claim 7, in which said frame of mind of a consumer is recorded to provide empirical evidence of the effectiveness of an advertising campaign.
10. A method as recited in claim 7, in which said frame of mind of a consumer is recorded and then analyzed to provide a sponsor with marketing information.
11. A method as recited in claim 7, in which said frame of mind of a consumer is recorded, analyzed and then combined with call data to provide a sponsor with demographic information.
12. A method as recited in claim 8, in which said precisely targeting promotion includes an offer to purchase specific goods associated with said spoken keyword.
13. A method as recited in claim 8, in which said precisely targeting promotion includes an offer to purchase specific services associated with said spoken keyword.
14. A method as recited in claim 8, in which said precisely targeting promotion includes an offer to obtain information associated with said spoken keyword.
15. A method as recited in claim 8, in which said precisely targeting promotion includes an offer to allow specific comments associated with said spoken keyword to be recorded.
16. A method as recited in claim 8, in which the deployment of said precisely targeting promotion is automatically altered in response to recognized trends in the detection of said spoken keyword.
17. A method as recited in claim 7, in which said frame of mind of a consumer is detected regarding a political campaign.
18. A method as recited in claim 7, in which said precisely targeting promotion that is triggered by the detection of said frame of mind pertains to a political campaign.