1. A method for treating Bethlem myopathy, Ullrich Congenital Muscular Dystrophy or Sorsby’s fundus dystrophy, comprising administering to a mammal a biglycan polypeptide, which biglycan polypeptide comprises an amino acid sequence that is at least about 90% identical to amino acids 38-368 of SEQ ID NO: 9, or a portion thereof, and which amino acid sequence has a biological activity of human biglycan.
2. The method of claim 1, wherein the biglycan polypeptide binds to Muscle-specific kinase (MuSK) on a mammalian cell.
3. The method of claim 1, wherein the biglycan polypeptide binds to a \u03b1-sarcoglycan andor \u03b3-sarcoglycan on a mammalian cell.
4. The method of claim 1, wherein the biglycan polypeptide binds to a collagen VI polypeptide on a mammalian cell.
5. The method of claim 1, wherein the biglycan polypeptide causes phosphorylation of sarcoglycans on a mammalian cell.
6. The method of claim 1, wherein the biglycan polypeptide causes an increase in utrophin levels in a mammalian cell.
7. The method of claim 1, wherein the biglycan polypeptide is derivatized with one or more glycosaminoglycan (GAG) side chains.
8. The method of claim 1, wherein the amino acid sequence of the biglycan polypeptide is at least about 95% identical to amino acids 38-368 of SEQ ID NO: 9.
9. The method of claim 1, wherein the biglycan polypeptide comprises amino acids 38-368 of SEQ ID NO: 9.
10. The method of claim 1, wherein the biglycan polypeptide consists of amino acids 38-368 of SEQ ID NO: 9.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A seed of rice cultivar L-206, wherein a representative sample of seed of said cultivar was deposited under ATCC Accession No. PTA-9822.
2. A rice plant, or a part thereof, produced by growing the seed of claim 1.
3. A tissue culture of cells produced from the plant of claim 2, wherein said cells of the tissue culture are produced from a plant part selected from the group consisting of leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip, pistil, anther, flower, stem, glumes and panicle.
4. A protoplast produced from the plant of claim 2.
5. A protoplast produced from the tissue culture of claim 3.
6. A rice plant regenerated from the tissue culture of claim 3, wherein the plant has all the morphological and physiological characteristics of cultivar L-206.
7. A method for producing an F1 hybrid rice seed, wherein the method comprises crossing the plant of claim 2 with a different rice plant and harvesting the resultant F1 hybrid rice seed.
8. A hybrid rice seed produced by the method of claim 7.
9. A hybrid rice plant, or a part thereof, produced by growing said hybrid seed of claim 8.
10. A method of producing an herbicide resistant rice plant wherein the method comprises transforming the rice plant of claim 2 with a transgene wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.
11. An herbicide resistant rice plant produced by the method of claim 10.
12. A method of producing an insect resistant rice plant wherein the method comprises transforming the rice plant of claim 2 with a transgene that confers insect resistance.
13. An insect resistant rice plant produced by the method of claim 12.
14. The rice plant of claim 13, wherein the transgene encodes a Bacillus thuringiensis endotoxin.
15. A method of producing a disease resistant rice plant wherein the method comprises transforming the rice plant of claim 2 with a transgene that confers disease resistance.
16. A disease resistant rice plant produced by the method of claim 15.
17. A method of producing a rice plant with modified fatty acid metabolism or modified carbohydrate metabolism wherein the method comprises transforming the rice plant of claim 2 with a transgene encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or encoding an antisense of stearyl-ACP desaturase.
18. A rice plant having modified fatty acid metabolism or modified carbohydrate metabolism produced by the method of claim 17.
19. A method of introducing a desired trait into rice cultivar L-206 wherein the method comprises:
(a) crossing an L-206 plant, wherein a representative sample of seed was deposited under ATCC Accession No. PTA-9822, with a plant of another rice cultivar that comprises a desired trait to produce progeny plants wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, modified fatty acid metabolism, modified carbohydrate metabolism and resistance to bacterial disease, fungal disease or viral disease;
(b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants;
(c) crossing the selected progeny plants with the L-206 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and the physiological and morphological characteristics of rice cultivar L-206 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three times to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of rice cultivar L-206 as listed in Table 1.
20. A plant produced by the method of claim 19, wherein the plant has the desired trait and all of the physiological and morphological characteristics of rice cultivar L-206 as listed in Table 1.
21. The plant of claim 20, wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.
22. The plant of claim 20, wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.
23. The plant of claim 20, wherein the desired trait is modified fatty acid metabolism or modified carbohydrate metabolism and said desired trait is conferred by a nucleic acid encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or encoding an antisense of stearyl-ACP desaturase.