1. An isolated or recombinant antibody, which is capable of specifically binding to interleukin (IL)-3R\u03b1 chain, wherein the antibody comprises:
(i) a light chain variable region (VL) comprising CDRs 1, 2 and 3 as set forth in SEQ ID NOs: 2, 3 and 4, respectively;
(ii) a heavy chain variable region (VH) comprising CDRs 1, 2 and 3 as set forth in SEQ ID NOs: 5, 6 and 7, respectively; and
(iii) a heavy chain constant region comprising amino acid substitutions S239D and I332E according to the EU numbering system of Kabat.
2. An isolated or recombinant humanized antibody, the antibody capable of specifically binding to interleukin (IL)-3R\u03b1 chain and comprising:
(i) a light chain variable region (VL) comprising an amino acid sequence according to SEQ ID NO: 8 and a heavy chain variable region (VH) comprising an amino acid sequence according to SEQ ID NO: 9; and
(ii) a heavy chain constant region comprising amino acid substitutions S239D and I332E according to the EU numbering system of Kabat.
3. The antibody of claim 1 or 2, wherein the heavy chain constant region comprises a sequence set forth between residues 121-450 (inclusive) of SEQ ID NO:
4. An isolated or recombinant humanized antibody, the antibody capable of specifically binding to IL-3R\u03b1 chain and comprising a light chain comprising a sequence set forth in SEQ ID NO: 13 and a heavy chain comprising a sequence set forth in SEQ ID NO: 11.
5. The antibody of any one of claims 1 to 4, which is a naked antibody.
6. A pharmaceutical composition comprising the antibody of any one of claims 1 to 5 and a pharmaceutically acceptable carrier, diluent or excipient.
7. A nucleic acid encoding the humanized antibody of any one of claims 1 to 5 or a heavy chain or light chain thereof.
8. A genetic construct comprising a nucleic acid encoding a heavy chain of the antibody of any one of claims 1 to 5 operably linked to a promoter and a nucleic acid encoding a light chain of the antibody of any one of claims 1 to 5 operably linked to a promoter.
9. An isolated cell expressing the humanized antibody of any one of claims 1 to 5 or a cell genetically modified to express the humanized antibody of any one of claims 1 to 5.
10. The cell of claim 9 comprising the nucleic acid of claim 7 or the genetic construct of claim 8.
11. A method for treating a disease or condition in a mammal, the method comprising administering the antibody of any one of claims 1 to 5 or the composition of claim 6 to a mammal in need thereof.
12. Use of the antibody of any one of claims 1 to 5 or the composition of claim 6 in medicine.
13. Use of the antibody of any one of claims 1 to 5 in the manufacture of a medicament for the treatment of a disease or condition in a mammal.
14. The antibody of any one of claims 1 to 5 or the composition of claim 6 for use in the treatment of a disease or condition in a mammal
15. The method of claim 11 or the use of claim 12 or 13 or the antibody or composition of claim 14, wherein the disease or condition is an IL-3R\u03b1-mediated disease or condition.
16. The method, use, antibody or composition of claim 15, wherein the disease or condition is cancer or an autoimmune or inflammatory condition.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A method of electronically specifying a construction item made up of constituent elements, comprising the steps of:
determining a number of constituent elements needed to specify said construction item;
storing information related to a first constituent element as a database object;
storing additional information related to additional constituent elements until the number of constituent elements matches the number of elements in said determining step; and
publishing said first constituent element and said additional constituent elements in XML format.
2. The method of claim 1, wherein said publication takes place over the Internet.
3. The method of claim 1, wherein said construction item is a constituent material of concrete.
4. The method of claim 1, wherein said construction item is a concrete recipe.
5. The method of claim 1, wherein said construction item is a construction standard test method.
6. The method of claim 1, wherein said first constituent element comprises a first quantifiable property.
7. The method of claim 6, wherein said first quantifiable property is stored in said database as a discrete value.
8. The method of claim 6, wherein said first quantifiable property is stored in said database as a continuous value.
9. The method of claim 6, wherein said first quantifiable property is stored in said database as dependent on another quantifiable property in the database.
10. The method of claim 6, wherein said first quantifiable property is stored in said database as independent of other quantifiable properties in the database.
11. The method of claim 6, further comprising the steps of:
creating a material specification by selecting appropriate database objects describing said test method; and
publishing said material specification over the Internet in XML format.
12. The method of claim 11, wherein said material specification is based on industry standards.
13. The method of claim 11, wherein said material specification is based on manufacturer specifications.
14. The method of claim 12, further comprising the steps of:
creating a material brand name based on industry defined product specifications; and
storing said brand name in a brand name data depository; and
publishing said brand name in XML format over the Internet.
15. The method of claim 11 further comprising the steps of:
verifying standards compliance for each material specification and
publishing a compliance report over the Internet in XML format.
16. An electronic method of specifying concrete, comprising the steps of:
receiving a specification for a concrete recipe, wherein each component of said recipe is based on an industry standard;
matching each of said components with a real-world material at a certain locality that satisfies said industry standard;
creating a concrete production mix by listing all of said real-world material; and
publishing said production mix in XML format over the Internet.
17. The method of claim 16, wherein said receiving step is comprised of:
receiving a specification for a concrete recipe, wherein each component in said recipe is based on a real-world material at a certain locality; and
creating a concrete basemix by converting each of said real-world materials into a standards-based material whose characteristics are satisfied by the real-world material.
18. A method for mixing a batch of concrete at a batch plant, comprising the steps of:
obtaining a mix recipe for the concrete;
sensing the external environmental conditions at the batch plant;
adjusting the mix recipe to account for said sensed environmental conditions; and
mixing said batch of concrete.
19. A method of producing concrete comprising the steps of:
selecting a basemix from a data repository, said basemix have a plurality of components;
instantiating said components into real world material; and
combining said real world materials.
20. An electronic system for specifying a production mix to be produced at a specified locality comprised of:
a database having a first production mix;
means for instantiating concrete mixes, wherein said means said is capable of converting said first production mix into a generic basemix, and further wherein said means is capable of converting said generic basemix into a second production mix said specified locality.