1. A work station for assembling multiple tire components onto a tire building drum having an axis of rotation; comprising:
a first set of measuring drums;
a second set of measuring drums;
lateral motion means for independently and controllably moving the first and second sets of measuring drums, respectively, in a direction parallel to the axis of rotation of the building drum; and
longitudinal motion means for independently and controllably moving the first and second sets of measuring drums in a direction perpendicular to the axis of rotation of the building drum,
wherein the first set of measuring drums are positioned laterally inside of the second set of measuring drums and the second set of measuring drums are movable towards the tire building drum so as to pass laterally outside of and around the first set of measuring drums.
2. A work station according to claim 1, including:
a first set of measuring drum carriages for the first set of measuring drums;
a second set of measuring drum carriages for the second set of measuring drums;
a first set of drum support bases for attaching the first set of measuring drums to the first set of measuring drum carriages;
a second set of drum support bases for attaching the second set of measuring drums to the second set of measuring drum carriages.
3. A work station according to claim 2, wherein:
the first set of measuring drum carriages associated with the first set of measuring drums are positioned laterally inside of the second set of measuring drum carriages associated with the second set of measuring drums.
4. A work station according to claim 2, wherein:
the lateral motion means comprise laterally oriented leadscrews and lateral leadscrew followers, with both the first and second measuring drum carriages connected to a lateral leadscrew follower.
5. A work station according to claim 2, wherein:
the longitudinal motion means comprise a longitudinally oriented leadscrew mounted to each of the first and second sets of measuring drum carriages and a longitudinal leadscrew follower associated with each longitudinally oriented leadscrew, each of the first and second sets of measuring drum support bases being connected to a longitudinal leadscrew follower.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An isolated mutant of human Follicle Stimulating Hormone (FSH) having FSH activity, wherein one N-glycosylation site has been introduced into the FSH alpha subunit by the mutation F17T.
2. An isolated mutant of human Follicle Stimulating Hormone (FSH) having FSH activity, wherein one N-glycosylation site has been introduced into the FSH alpha subunit by the mutation E56N.
3. An isolated mutant of human Follicle Stimulating Hormone (FSH) having FSH activity, wherein one N-glycosylation site has been introduced into the FSH beta subunit by the mutation A70N.
4. An isolated mutant of human Follicle Stimulating Hormone (FSH) having FSH activity, wherein one N-glycosylation site has been introduced into the FSH beta subunit by the mutation V78N.
5. An isolated mutant of human Follicle Stimulating Hormone (FSH) having FSH activity, wherein one N-glycosylation site has been introduced into the FSH beta subunit by the mutation G100N.
6. An isolated mutant of human Follicle Stimulating Hormone (FSH) mutant having FSH activity, wherein one N-glycosylation site has been introduced into the FSH beta subunit by the mutations D41NA43T.
7. A composition comprising the FSH mutant according to any one of claims 1-6 and at least one pharmaceutically acceptable carrier or excipient.
8. A method of treating an infertile human, comprising administering to the human in need thereof an effective amount of the human FSH mutant according to any one of claims 1-6.
9. A method of stimulating folliculogenesis in a human, comprising administering to the human an effective amount of the FSH mutant according to any one of claims 1-6.
10. A method of inducing ovarian hyperstimulation in a human, comprising administering to the human an effective amount of the FSH mutant according to any one of claims 1-6.
11. An isolated nucleic acid encoding the FSH mutant of claim 1.
12. An isolated nucleic acid encoding the FSH mutant of claim 2.
13. An isolated nucleic acid encoding the FSH mutant of claim 3.
14. An isolated nucleic acid encoding the FSH mutant of claim 4.
15. An isolated nucleic acid encoding the FSH mutant of claim 5.
16. An isolated nucleic acid encoding the FSH mutant of claim 6.
17. An expression vector comprising the nucleic acid according to any one of claims 11-16.
18. An isolated host cell transformed with a vector according to claim 17.
19. The isolated host cell of claim 18 which is eukaryotic.
20. The isolated host cell of claim 19 which is mammalian.
21. A method for producing a FSH mutant, comprising subjecting the isolated host cell according to claim 18 to cultivation under conditions conducive for expression of the FSH mutant to thereby produce the FSH mutant.
22. A method for producing a FSH mutant, comprising subjecting the isolated host cell according to claim 19 to cultivation under conditions conducive for expression of the FSH mutant to thereby produce the FSH mutant.
23. A method for producing a FSH mutant, comprising subjecting the isolated host cell according to claim 20 to cultivation under conditions conducive for expression of the FSH mutant to thereby produce the FSH mutant.