1. A method for staining, comprising:
first immersing a sample in an eosin solution; and
then immersing the sample in a hematoxylin solution.
2. The method of claim 1, further comprising fixing the sample in a watery alcohol solution.
3. The method of claim 2, wherein the watery alcohol solution is 80 percent alcohol.
4. The method of claim 1, wherein the sample is immersed in the eosin solution for ten intervals.
5. The method of claim 1, further comprising immersing the sample in tap water.
6. The method of claim 1, wherein the sample is immersed in the hematoxylin solution for 20 intervals.
7. The method of claim 5, wherein the sample is immersed in tap water for ten intervals.
8. The method of claim 1, further comprising air drying the sample.
9. The method of claim 1, further comprising immersing the sample in an eosin solution after first immersing the sample in the eosin solution and secondly immersing the sample in the hematoxylin solution.
10. The method of claim 9, wherein the second immersion of the sample in eosin is for ten intervals.
11. The method of claim 9, further comprising immersing the sample in tap water after the second immersion of the sample in eosin.
12. The method of claim 11, wherein immersing the sample in tap water after the second immersion is for ten intervals.
13. A method for staining, comprising:
first immersing a sample in an eosin solution;
then immersing the sample in a water solution;
then immersing the sample in a hematoxylin solution;
then immersing the sample in a water solution;
then immersing the sample in an eosin solution;
then immersing the sample in a water solution; and
then air drying the sample.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An airtight device for a container cap, comprising:
a container having a pouring portion defined therein;
a filling tube selectively inserted into the container via the pouring portion, the filling tube having an annular flange radially and outwardly extending from one end thereof;
an airtight element air-tightly mounted to the annular flange of the filling tube;
a cap mounted to the pouring portion in the container, a through hole defined in the cap to allow the filling tube to extend through the cap during filling; and
a shelter mounted between the annular flange and the cap for closing the through hole in the cap after filling.
2. The airtight device as claimed in claim 1, wherein the cap has multiple bosses formed on an inner periphery thereof and the multiple bosses surround the airtight element and engage an outer periphery of the airtight element such that the filling tube is connected to the cap when the cap is mounted to the pouring portion.
3. The airtight device as claimed in claim 1, wherein the shelter has a raised portion centrally formed thereon, the raised portion having a diameter corresponding to that of the through hole in the cap.
4. The airtight device as claimed in claim 1 further comprising an airtight washer mounted between the cap and the shelter.
5. The airtight device as claimed in claim 1, wherein the shelter has a diameter smaller than that of the airtight element.
6. The airtight device as claimed in claim 1, wherein the shelter has multiple protrusions radially and outwardly extending therefrom, the multiple protrusions defining a circumference that has a diameter equal to that of the airtight element such that the shelter is easily guided to a central position when the cap is screwed onto the pouring portion.
7. The airtight device as claimed in claim 1, wherein the container, the cap and the shelter are made of metal.