1. A method comprising:
classifying a business entity;
answering questions pre-populated with answers and replacing said pre-populated answers with different answers when said pre-populated answers are incorrect; and
performing at least one of a return-on-investment (\u201cROI\u201d) analysis of equipment prior to a purchase of said equipment, generating an output of said analysis, storing a result of said answered questions, and transmitting said answered questions towards a server.
2. The method of claim 1, wherein said ROI analysis comprises:
acquiring costs for at least one piece of first equipment from a first provider and for at least one piece of second equipment from a second provider;
comparing said costs of said at least one piece of first equipment with said at least one piece of second equipment.
3. The method of claim 1, wherein said ROI analysis comprises:
estimating costs associated with a user’s current equipment.
4. The method of claim 1, wherein said ROI analysis comprises:
estimating costs associated with at least one of an equipment purchase, an implementation of said equipment, operating costs associated with said equipment, and risksbenefits associated with said equipment.
5. The method of claim 1, wherein said ROI analysis comprises:
altering a classification of a user’s resources based upon a priority selection.
6. The method of claim 1, further comprising:
receiving, from said server, data updates; and
storing said data updates in a database.
7. A computer-readable medium having stored thereon a plurality of instructions, the plurality of instructions including instructions which, when executed by a processor, cause the processor to perform the steps comprising:
classifying a business entity;
answering questions pre-populated with answers and replacing said pre-populated answers with different answers when said pre-populated answers are incorrect; and
performing at least one of a return-on-investment (\u201cROI\u201d) analysis of equipment prior to a purchase of said equipment, generating an output of said analysis, storing a result of said answered questions, and transmitting said answered questions towards a server.
8. The computer-readable medium of claim 7, wherein said ROI analysis comprises:
acquiring costs for at least one piece of first equipment from a first provider and for at least one piece of second equipment from a second provider;
comparing said costs of said at least one piece of first equipment with said at least one piece of second equipment.
9. The computer-readable medium of claim 7, wherein said ROI analysis comprises:
estimating costs associated with a user’s current equipment.
10. The computer-readable medium of claim 7, wherein said ROI analysis comprises:
estimating costs associated with at least one of an equipment purchase, an implementation of said equipment, operating costs associated with said equipment, and risksbenefits associated with said equipment.
11. The computer-readable medium of claim 7, wherein said ROI analysis comprises:
altering a classification of a user’s resources based upon a priority selection.
12. The computer-readable medium of claim 7, further comprising:
receiving, from said server, data updates; and
storing said data updates in a database, wherein said database comprises pre-populated answers.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of administering a protein or peptide to a subject, the method comprising administering to the subject a chimeric flavivirus comprising structural sequences of a first flavivirus and non-structural sequences of a yellow fever virus, wherein the genome of said chimeric flavivirus comprises an internal ribosome entry site and a transgene located in the 3\u2032-untranslated region of the flavivirus.
2. The method of claim 1, wherein the chimeric flavivirus comprises pre-membrane and envelope sequences of said first flavivirus, and capsid and non-structural sequences of said yellow fever virus.
3. The method of claim 1, wherein the first flavivirus is selected from the group consisting of Japanese encephalitis, Dengue-1, Dengue-2, Dengue-3, Dengue-4, Murray Valley encephalitis, St. Louis encephalitis, West Nile, Kunjin, Rocio encephalitis, Ilheus, Tick-borne encephalitis, Central European encephalitis, Siberian encephalitis, Russian Spring-Summer encephalitis, Kyasanur Forest Disease, Omsk Hemorrhagic fever, Louping ill, Powassan, Negishi, Absettarov, Hansalova, Apoi, and Hypr viruses.
4. The method of claim 1, wherein the first flavivirus is a Japanese encephalitis virus.
5. The method of claim 1, wherein the flavivirus comprises a deletion within the 3\u2032-untranslated region of the flavivirus.
6. The method of claim 1, wherein the transgene encodes a vaccine antigen.
7. The method of claim 6, wherein the vaccine antigen is derived from an infectious agent.
8. The method of claim 7, wherein the infectious agent is an influenza virus.
9. The method of claim 8, wherein the vaccine antigen is selected from the group consisting of hemagglutinin, neuraminidase, or an immunogenic fragment thereof.
10. The method of claim 6, wherein the vaccine antigen is a tumor-associated antigen.
11. A nucleic acid molecule encoding a chimeric flavivirus comprising structural sequences of a first flavivirus and non-structural sequences of a yellow fever virus, wherein the genome of said chimeric flavivirus comprises an internal ribosome entry site and a transgene located in the 3\u2032-untranslated region of the chimeric flavivirus.
12. The nucleic acid molecule of claim 11, wherein the transgene encodes an influenza antigen or an immunogenic fragment thereof.