What is claimed is:
1. A monoclonal antibody that specifically binds to an isolated A-type substance obtainable from human liver or placenta, wherein the substance is a cyclitol-containing carbohydrate comprising a Zn2 ion and has the biological activity of regulating lipogenic activity and inhibiting cAMP dependent protein kinase.
2. The monoclonal antibody of claim 1 wherein the substance comprises phosphate.
3. A hybridoma producing the monoclonal antibody of claim 1.
4. A pharmaceutical composition comprising the monoclonal antibody of claim 1 in combination with a pharmaceutically acceptable carrier.
5. The monoclonal antibody of claim 1, wherein the monoclonal antibody is an antagonist having the property of:
a) inhibiting the release of the A-type substance;
b) binding to the A-type substance and thereby reducing its level; andor
c) reducing a biological activity of the A-type substance.
6. The monoclonal antibody of claim 1, wherein the monoclonal antibody is linked, directly or indirectly, to a label.
7. The monoclonal antibody of claim 1, wherein the monoclonal antibody is immobilized on a solid phase.
8. An immunoassay method comprising:
a) contacting a biological sample with the monoclonal antibody of claim 1 under suitable conditions for specific binding of the monoclonal antibody to A-type substance present in the sample, if any; and
b) determining whether the monoclonal antibody binds specifically to the sample.
9. The immunoassay method of claim 8, additionally comprising measuring the amount of specific binding as an indication of the concentration of the A-type substance in the sample.
10. The immunoassay method of claim 9, additionally comprising determining the concentration of one or more P-type inositolphosphoglycans (IPGs) and then determining the ratio of the concentration of P-type IPG(s) to the concentration of the A-type substance determined in the immunoassay method.
11. A monoclonal antibody that specifically binds to a A-type cyclitol-containing carbohydrate substance comprising a Zn2 ion, wherein the substance has the biological activity of regulating lipogenic activity and inhibiting cAMP dependent protein kinase and:
(a) a molecular weight determined using negative mode MALDI mass spectroscopy as shown in tables 3 and 4, or a molecular weight related to one of the molecular weights set out in tables 3 and 4 by the addition or subtraction of one or more structure units of about 211 mz; or,
(b) a molecular weight determined using positive mode MALDI mass spectroscopy as shown in table 5, or a molecular weight related to one of the molecular weights set out in table 5 by the addition or subtraction of one or more structure units of about 211 mz.
12. The monoclonal antibody of claim 11 wherein the substance comprises phosphate.
13. A hybridoma producing the monoclonal antibody of claim 11.
14. A pharmaceutical composition comprising the monoclonal antibody of claim 11 in combination with a pharmaceutically acceptable carrier.
15. The monoclonal antibody of claim 11, wherein the monoclonal antibody is an antagonist having the property of:
a) inhibiting the release of the A-type substance;
b) binding to the A-type substance and thereby reducing its level; andor
c) reducing a biological activity of the A-type substance.
16. The monoclonal antibody of claim 11, wherein the monoclonal antibody is linked, directly or indirectly, to a label.
17. The monoclonal antibody of claim 11, wherein the monoclonal antibody is immobilized on a solid phase.
18. An immunoassay method comprising:
a) contacting a biological sample with the monoclonal antibody of claim 11 under suitable conditions for specific binding of the monoclonal antibody to A-type substance present in the sample, if any; and
b) determining whether the monoclonal antibody binds specifically to the sample.
19. The immunoassay method of claim 18, additionally comprising measuring the amount of specific binding as an indication of the concentration of the A-type substance in the sample.
20. The immunoassay method of claim 19, additionally comprising determining the concentration of one or more P-type inositolphosphoglycans (IPGs) and then determining the ratio of the concentration of P-type IPG(s) to the concentration of the A-type substance determined in the immunoassay method.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An angle testing apparatus for testing whether a mounting angle between a first object and a second object is in a desired angle range or not, the second object having a mounting portion for mounting the first object, the angle testing apparatus comprising:
a workbench configured for loading the second object thereon;
a fixing pole extending up from the workbench;
a triggering member rotatably mounted to the fixing pole, with a first contact portion and a second contact portion located at opposite sides of the fixing pole, the first contact portion configured for contacting the mounting portion of the second object;
a pair of first and second contact poles fixed at predetermined positions on the workbench respectively according to a minimum value and a maximum value of the desired angle range, for contacting the second contact portion of the triggering member when the mounting angle is not in the desired angle range;
a controller electrically connected to the fixing pole and the contact poles configured to send a signal based on connecting status between the second contact portion of the triggering member and the contact poles when the second object is moved on the workbench; and
an indicating assembly electrically connected to the controller to indicate the testing result according to the signal.
2. The angle testing apparatus as described in claim 1, wherein the triggering member defines a pivot hole in an intersection of the first and second contact portions thereof, for rotatably fitting about the fixing pole.
3. The angle testing apparatus as described in claim 1, further comprising a locating member installed on the workbench, wherein the locating member comprises a movable locating portion configured for locating the second object together with the first contact portion of the triggering member.
4. The angle testing apparatus as described in claim 3, wherein the locating member further comprises a limiting plate immovably fixed to the workbench, a pin slidably passing through the limiting plate, and an elastic member fitting about the pin and elastically located between the limiting plate and an end of the pin, the locating portion is fixed to an opposite end of the pin.
5. The angle testing apparatus as described in claim 4, wherein an installing hole is defined in the workbench, an upper part of the locating portion of the locating member extends through the installing hole.
6. The angle testing apparatus as described in claim 1, further comprising a photosensitive switch configured for generating a start signal when the second object is loaded on the workbench and sending the start signal to the controller.
7. The angle testing apparatus as described in claim 6, wherein the second contacting portion of the triggering member is located between the first and second contact poles, the indicator assembly indicates the mounting angle is smaller than or equal to a minimum value of the desired angle range when the second contacting portion contacts and is electrically connected to the first contact pole, and indicates the mounting angle is larger than or equal to a maximum value of the desired angle range when the contacting portion contacts and is electrically connected to the second contact pole.
8. An angle testing apparatus for testing a mounting angle between a first line and a second line extending through an object, the lines crossing with each other at different planes, the angle testing apparatus comprising:
a workbench for loading the object in such a manner that the object is slidable on the workbench along one of the lines;
a fixing pole fixed on the workbench, a first predetermined voltage level being applied to the fixing pole;
at least one contact pole fixed on a predetermined position of the workbench, a second predetermined voltage level being applied to said at least one contact pole;
a triggering member rotatably mounted to the fixing pole and matchable with the other one of the lines of the object, the triggering member being rotatable about the fixing pole, when being pushed by the object, to selectively electrically connect or disconnect the fixing pole with the at least one contact pole based on the mounting angle of the object; and
an indicator for giving out instruction according to the connection of the fixing pole, the at least one contact pole, and the triggering member.
9. The angle testing apparatus as described in claim 8, wherein when the fixing pole, said at least one contact pole, and the triggering member are electrically connected the instruction is given out to indicate that the mounting angle of the object is unqualified.
10. The angle testing apparatus as described in claim 8, wherein the predetermined position, where said at least one contact pole is fixed, is determined according to a desired maximum andor minimum tolerance of the angle.
11. The angle testing apparatus as described in claim 8, wherein the triggering member comprises two contact portions respectively configured for contacting the object and said at least one contact pole.
12. The angle testing apparatus as described in claim 8, further comprising a controller configured for providing the predetermined voltage levels for the fixing pole and said at least one contact pole and sending a signal according to the connection of the fixing pole, the at least one contact pole, and the triggering member to the indicator.
13. The angle testing apparatus as described in claim 8, further comprising a locating member slidably installed on the workbench, the object is clamped between the locating member and the triggering member when fixed on the workbench.
14. The angle testing apparatus as described in claim 13, wherein the locating member comprises:
a limiting plate immovably fixed on a first side of the workbench;
a pin slidably penetrating the limiting plate on the first side of the platform;
a locating portion fixed to the pin and penetrating the workbench from the first side to a second side thereof configured to locate the object; and
an elastic member sleeving the pin on the first side of the workbench, two ends of the elastic member being respectively fixed to the limiting plate and the pin.