1. A method of detecting CD34 negative hematopoietic stem cells in a blood sample, comprising:
removing CD34 positive cells from the blood sample thereby producing a modified blood sample;
extracting CD133 positive cells from the modified blood sample thereby producing a fraction comprising CD34 negative hematopoietic stem cells; and
analyzing the fraction with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.
2. The method of claim 1, wherein the removing of the CD34 positive cells comprises treating the blood sample with CD34 antibodies.
3. The method of claim 2, wherein the CD34 antibodies are fluorescently labeled.
4. The method of claim 3, wherein the CD34 antibodies are fluorescently labeled with fluorescein isothiocyanate.
5. The method of claim 1, wherein the removing of the CD34 positive cells comprises treating the blood sample with magnetic beads, each of which is bound to a fluorescently labeled CD34 antibody.
6. The method of claim 5, further comprising separating a portion comprising the CD34 positive cells and the magnetic beads from the modified blood sample.
7. The method of claim 1, wherein the extracting of the CD133 positive cells from the modified blood sample comprises treating the modified blood sample with CD133 antibodies.
8. The method of claim 7, wherein the CD133 antibodies are fluorescently labeled.
9. The method of claim 8, wherein the CD133 antibodies are fluorescently labeled with R-phycoerythrin.
10. The method of claim 1, wherein the extracting of the CD133 positive cells from the modified blood sample comprises treating the modified blood sample with magnetic beads, each of which is bound to a fluorescently labeled CD133 antibody, wherein the fraction further comprises the magnetic beads.
11. The method of claim 10, further comprising separating the fraction comprising the CD34 negative hematopoietic stem cells and the magnetic beads from a residual portion of the modified blood sample.
12. The method of claim 1, wherein the fraction further comprises CD133 positive cells and CD34 positive hematopoietic stem cells.
13. The method of claim 1, wherein the automated hematology analyzer comprises a SYSMEX SE-9000 analyzer.
14. The method of claim 1, wherein the automated hematology analyzer comprises a SYSMEX XE-2100 analyzer.
15. The method of claim 1, further comprising detecting the CD34 positive cells removed from the blood sample.
16. The method of claim 15, wherein the CD34 positive cells are detected with an automated hematology analyzer.
17. The method of claim 16, wherein the automated hematology analyzer comprises a SYSMEX SE-9000 analyzer.
18. The method of claim 16, wherein the automated hematology analyzer comprises a SYSMEX XE-2100 analyzer.
19. The method of claim 1, wherein the blood sample comprises cells selected from the group consisting of peripheral blood cells, bone marrow cells, cord blood cells, and combinations thereof.
20. The method of claim 1, wherein the blood sample comprises G-CSF mobilized peripheral blood cells.
21. The method of claim 1, further comprising producing a scattergram comprising a hematopoietic progenitor cell region, wherein a presence of the CD34 negative hematopoietic stem cells is identifiable therein.
22. The method of claim 1, wherein the detecting of the CD34 negative hematopoietic stem cells in the blood sample contributes to clinical monitoring of a treatment selected from the group consisting of hematopoiesis, stem cell mobilization, chemotherapy, radiation therapy, apheresis, and combinations thereof.
23. A method of detecting CD34 negative hematopoietic stem cells in a blood sample, comprising:
treating the blood sample with CD34 antibodies to produce a modified blood sample substantially depleted of CD34 positive cells;
treating the modified blood sample with CD133 antibodies to obtain a fraction comprising CD133 positive cells and CD34 negative hematopoietic stem cells; and
analyzing the fraction with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.
24. A method of detecting CD34 hematopoietic stem cells in a blood sample, comprising:
treating the blood sample with CD34 antibodies to produce a modified blood sample substantially depleted of CD34 positive cells and a fraction of the blood sample comprising CD34 positive cells removed from the blood sample;
treating the modified blood sample with CD133 antibodies to obtain a fraction of the modified blood sample comprising CD133 positive cells and CD34 negative hematopoietic stem cells;
analyzing the fraction of the blood sample with an automated hematology analyzer, thereby detecting the CD34 positive cells; and
analyzing the fraction of the modified blood sample with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A utensil for removing edible fruit from the rind of a fruit comprising:
a) a handle member;
b) a base member extending from the handle member;
c) a cutting blade rotatably mounted to the base member;
d) a spring mounted to the base member and the cutting blade so that the cutting blade can be reversibly rotated between a first position and a second position; and
e) a plurality of interconnected toothed sprockets rotatably mounted to the base member and to the cutting-blade;
wherein the utensil is placed in contact with a cutting surface and the handle member is rotated and the plurality of interconnected toothed sprockets are engaged so that cutting blade engages to allow the fruit to be cut from the rind to a user-selected depth.
2. The utensil of claim 1 wherein the base member comprises a first arm and a second arm that are generally parallel and the cutting blade is mounted tranversely between the first and second parallel arms.
3. The utensil of claim 2 wherein the spring is mounted to the first arm.
4. The utensil of claim 3 wherein the plurality of interconnected toothed sprockets are mounted to a rotatable shaft in the second arm.
5. The utensil of claim 1 wherein one of the plurality of interconnected toothed sprockets is formed as approximately one-fourth of a circle with two flat surfaces wherein one of the flat surfaces is adapted to contact the cutting surface during use and the handle member is rotated relative to the cutting surface to vary the depth of the cut and the second flat surface has a stop mounted thereon to prevent movement of the utensil beyond a predetermined position.
6. The utensil of claim 5 wherein the plurality of interconnected toothed sprockets comprises three toothed sprockets.