1. An electrophoretic chip having a trench-type flow path allowing therethrough electrophoretic migration of a sample to be subjected to mass analysis, said chip comprising:
a reservoir structure in adjacent to said flow path;
a substrate forming the bottom surface of said flow path and the bottom surface of said reservoir structure; and
a frame component provided on said substrate so as to form the sidewall of said flow path and the sidewall of said reservoir structure,
wherein said frame component has at least a part of openings in said flow path,
said substrate and said frame component are separable,
said substrate has a plurality of cylindrical components at least on the bottom surface of said flow path, and
said reservoir structure and said flow path are communicated while placing, in between, a region having an array of said cylindrical components formed therein.
2. The electrophoretic chip as claimed in claim 1,
wherein at least a part of either one of said frame component and said substrate is composed of a material having adhesiveness to the other.
3. The electrophoretic chip as claimed in claim 2,
wherein said material having adhesiveness is a gel-like material containing either one of a silicon polymer and an acryl polymer.
4-10. (canceled)
11. The electrophoretic chip as claimed in claim 1,
wherein said cylindrical components contain those different from each other in either of the size and geometry.
12. The electrophoretic chip as claimed in claim 1,
wherein said cylindrical components are arranged according to different densities.
13. (canceled)
14. (canceled)
15. An electrophoretic chip having a trench-type flow path allowing therethrough electrophoretic migration of a sample to be subjected to mass analysis, said chip comprising:
a substrate forming the bottom surface of said flow path; and
a frame component provided on said substrate so as to form the sidewall of said flow path,
wherein said frame component is formed by using a gel-like material containing either one of a silicon polymer and an acryl polymer, and
said substrate and said frame component are separable.
16. The electrophoretic chip as claimed in claim 1,
wherein either one of said frame component and said substrate has an alignment mark used for alignment between said frame component and said substrate.
17. The electrophoretic chip as claimed in claim 16,
wherein said alignment mark includes a pair of projection and hole engageable with each other,
said projection is provided to either one of said frame component and said substrate, and
said hole is provided to the other corresponding to said projection.
18. The electrophoretic chip as claimed in claim 1,
wherein either one of said frame component and said substrate fits into the other.
19. The electrophoretic chip as claimed in claim 1,
wherein said sidewall of said flow path has a tapered portion.
20. The electrophoretic chip as claimed in claim 1,
wherein the bottom surface of said flow path has a maximum difference of height of 100 \u03bcm or smaller.
21. A method of using the electrophoretic chip claimed in claim 1 as a target plate for mass analysis, said method comprising:
a first step which includes:
injecting a solution of said sample into said flow path;
applying voltage along said flow path to thereby fractionate a solution of said sample in said flow path; and
drying said sample, and
a second step setting said sample to a laser desorptionionization mass spectrometer, irradiating laser
to said sample while moving the bottom surface of said flow path relative to the region of laser irradiation, to thereby detect mass of said sample,
wherein said first step is carried out while combining said substrate and said frame component,
said second step is carried out while detaching said frame component from said substrate, and
said first step is carried out so as to bring the top surface of the solution of said sample retained by said flow path into contact with a gas.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting essentially of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid, said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
2. The fluid composition according to claim 1 wherein said refrigerator oil has a pour point of \u221220\xb0 C. to \u221280\xb0 C.
3. The fluid composition according to claim 1 wherein said 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid are in a molar ratio of 1:1.
4. The fluid composition according to claim 1 which additionally contains 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters.
5. The fluid composition according claim 1 which additionally contains at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters.
6. The fluid composition according to claim 1 which additionally contains at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators.
7. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil being as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid, said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
8. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; and 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
9. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; and at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
10. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; and 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
11. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; and not more than 10% by weight of at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
12. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes; and 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
13. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil being as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters; and 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
14. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil being as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters; and not more than 10% by weight of at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
15. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes; and 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
16. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil being as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters; and not more than 10% by weight of at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
17. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes; and not more than 10% by weight of at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
18. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes; 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters; and 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
19. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and 1\u2013500 parts by weight based on 100 parts by weight of said refrigerant of a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes; 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters; and not more than 10% by weight of at least one additive selected from the group consisting\u2014of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
20. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters; 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters; and not more than 10% by weight of at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
21. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes; 0.1\u20135%, by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters; and not more than 10% by weight of at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.
22. A fluid composition for a refrigerator, which comprises a chlorine-free fluorocarbon refrigerant and a refrigerator oil, said refrigerator oil consisting of as a major component a tetraester of pentaerythritol of formula (1)
with both 2-ethylhexanoic acid and 3,5,5-trimethylhexanoic acid; at least one conventional oil selected from the group consisting of paraffinic mineral oils, naphthenic mineral oils, poly\u03b1-olefins and alkylbenzenes; 0.1\u20135% by weight based on the total amount of said refrigerator oil of at least one epoxy compound, said epoxy compound being a member selected from the group consisting of phenylglycidyl ether epoxy compounds, alkylphenylglycidyl ether epoxy compounds, glycidyl ester epoxy compounds, and epoxidized fatty acid monoesters; 0.1\u20135.0% by weight based on the total amount of said refrigerator oil of at least one phosphorus compound selected from the group consisting of phosphoric esters, acid phosphoric esters, amine salts of acid phosphoric esters, chlorinated phosphoric esters, and phosphorous esters; and not more than 10% by weight of at least one additive selected from the group consisting of phenol antioxidants, amine antioxidants, wear resistant additives, extreme pressure agents, oiliness improvers, antifoaming agents and metal inactivators and said refrigerator oil exhibiting a pour point not higher than \u221210\xb0 C.