1. A method of controlling flow of frame based data transmitted from a local frame based data channel interface over a synchronous digital network, comprising steps of:
receiving said frame based data at a first rate;
configuring a buffer to receive said frame based data;
predetermining a data amount threshold level for said buffer;
with respect to said threshold level, monitoring an amount of said transmitted frame based data that has been received; and
in response to said step of monitoring said amount of said data received, generating a signal, wherein said signal is configurable to adapt said first rate of transmission from said frame based data channel interface, to a second rate.
2. A method according to claim 1, wherein said data is received directly from an Ethernet local area network.
3. A method according to claim 1, wherein said network comprises a synchronous digital hierarchy (SDH) network.
4. A method according to claim 1, wherein said network comprises a SONET network.
5. A method according to claim 1, wherein said step of receiving said frame based data comprises receiving one or more pause frames generated by a local area network switch.
6. A method according to claim 1, wherein said buffer comprises data storage locations configurable to store at least one data frame.
7. A method according to claim 1, wherein said buffer comprises a size equal to a number of maximum length Ethernet frames, said number being selectable from the set comprising 4 and 6.
8. A method according to claim 1, wherein said buffer is configured as a first in first out (FIFO) queue.
9. A method according to claim 1, wherein said buffer is configured as a circular buffer.
10. A method according to claim 1, wherein said step of monitoring said amount of transmitted frame based data received, comprises determining if said amount is less than said threshold levels.
11. A method according to claim 1, wherein said signal is generated and sent to said local interface if said amount of said frame based data received is not less than said threshold level.
12. A method according to claim 1, wherein if said amount of said frame based data received is not less than said threshold level, then a decision to generate said signal is made substantially immediately.
13. A method according to claim 1, wherein if said amount of said frame based data is not less than said threshold level then said signal is transmitted to said local interface upon another frame, currently being transmitted to said local interface, being completed.
14. A method according to claim 1, wherein said signal comprises a pause frame specifying a predetermined time interval for inhibiting further transmissions from said local frame based data channel interface.
15. A method according to claim 1, wherein said buffer comprises, above said threshold level, an amount of data storage capacity equal to the size of two maximum length Ethernet frames.
16. A method of controlling flow of frame based data, received from a synchronous digital network, to a frame based data channel interface, comprising steps of:
receiving said frame based data at a first rate;
configuring a buffer to receive said frame based data;
predetermining a data amount threshold level for said buffer;
with respect to said threshold level, monitoring an amount of said frame based data received; and
in response to said step of monitoring said amount of said data received, generating a signal, wherein said signal is configurable to adapt said first rate of transmission of said frame based data over said synchronous digital network to a second rate.
17. A method according to claim 16, wherein said network comprises a synchronous digital hierarchy network.
18. A method according to claim 16, wherein said network comprises a SONET network.
19. A method according to claim 16, wherein said step of receiving said frame based data comprises receiving one or more pause frames.
20. A method according to claim 16, wherein said buffer comprises data storage locations configurable to store at least one data frame.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An isolated peptide selected from the group consisting of:
a) a peptide consisting of SEQ ID NO:2;
b) a peptide consisting of an amino acid sequence that differs from SEQ ID NO:2 by at least one substitution of an amino acid residue at a position of SEQ ID NO:2 selected from the group consisting of: 1, 2, 5, 6, 9, 10, 11, 12, 13, and 14, wherein the peptide promotes myoblast differentiation; and
c) a peptide consisting of an amino acid sequence that differs from SEQ ID NO:2 by substitutions at positions 1, 5, 6, 9, 11, and 14, wherein the peptide promotes myoblast differentiation;
wherein the amino acid residues substituted into positions 1, 2, 5, 6, 9, 10, 11, 12, 13, and 14 of SEQ ID NO:2 are those residues found at positions 1, 2, 5, 6, 9, 10, 11, 12, 13, and 14 in any of SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, and SEQ ID NO: 24, respectively.
2. The isolated peptide of claim 1, wherein the peptide consists of an amino acid sequence that differs from SEQ ID NO:2 by one substitution, deletion or insertion of an amino acid residue at a position of SEQ ID NO:2 selected from the group consisting of: 1, 2, 5, 6, 9, 10, 11 and 12.
3. The isolated peptide of claim 1, wherein the peptide consists of SEQ ID NO:2.
4. The isolated peptide of claim 1, wherein the peptide comprises a modification selected from the group consisting of farnesylation, carboxymethylation, geranyl-geranylation, and complexing with a lipid carrier.
5. A composition comprising the isolated peptide of claim 1 and a pharmaceutically acceptable carrier.
6. A protein comprising a protein that is chemically or recombinantly conjugated to a agent that increases the half-life of the protein in cardiac or skeletal muscle tissue, wherein the protein is selected from the group consisting of:
a) a protein comprising an amino acid sequence represented by SEQ ID NO:4;
b) a protein consisting of at least 600 consecutive amino acids of SEQ ID NO:4, wherein the protein promotes myoblast differentiation; and
c) a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:4, wherein the protein promotes myoblast differentiation.
7. A method to identify compounds that regulate myoblast activation and differentiation, comprising:
a) contacting a prelamin A protein (SEQ ID NO:4) or a prelamin A pre peptide (SEQ ID NO:2) with a test compound under conditions suitable for binding of the prelamin A protein or prelamin A pre peptide by the test compound;
b) detecting binding of the prelamin A protein or prelamin A pre peptide by the test compound;
c) contacting myoblast with test compound of step b) that bind SEQ ID NO:2 or SEQ ID NO:4; and
d) detecting myoblast differentiation in the presence and absence of said test compound, thereby identifying said test compound that regulates myoblast differentiation.
8. The isolated peptide of claim 1, wherein the peptide consists of an amino acid sequence that differs from SEQ ID NO:2 by at least one substitution of an amino acid residue at a position of SEQ ID NO:2 selected from the group consisting of: 1, 2, 5, 6, 9, 10, 11, 12, 13, and 14, wherein the peptide promotes myoblast differentiation.
9. The isolated peptide of claim 1, wherein the peptide consists of an amino acid sequence that differs from SEQ ID NO:2 by at least one substitution of an amino acid residue at a position of SEQ ID NO:2 selected from the group consisting of: 1, 2, 5, 6, 9, 10, and 11, wherein the peptide promotes myoblast differentiation.
10. The protein of claim 6, wherein the protein comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:4.
11. The protein of claim 6, wherein the protein comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:4.
12. The protein of claim 6, wherein the protein comprises an amino acid sequence represented by SEQ ID NO:4.
13. The protein of claim 6, wherein the protein comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:4.
14. A method to promote myoblast differentiation, comprising administering to a myoblast stem cell the isolated peptide of claim 1.
15. The method of claim 14, wherein the isolated peptide consists of SEQ ID NO:2.