1. An isolated polypeptide comprising the amino acid sequence shown as SEQ ID NO:483.
2. An isolated polypeptide comprising the amino acid sequence shown as SEQ ID NO:483, lacking its associated signal peptide.
3. An isolated polypeptide comprising the amino acid sequence of the polypeptide encoded by the full-length coding sequence of the cDNA deposited under ATCC accession number 209859.
4. A chimeric polypeptide comprising a polypeptide according to claim 1 fused to a heterologous polypeptide.
5. The chimeric polypeptide of claim 4, wherein said heterologous polypeptide is an epitope tag or an Fc region of an immunoglobulin.
6. A chimeric polypeptide comprising a polypeptide according to claim 2 fused to a heterologous polypeptide.
7. The chimeric polypeptide of claim 6, wherein said heterologous polypeptide is an epitope tag or an Fc region of an immunoglobulin.
8. A chimeric polypeptide comprising a polypeptide according to claim 3 fused to a heterologous polypeptide.
9. The chimeric polypeptide of claim 8, wherein said heterologous polypeptide is an epitope tag or an Fc region of an immunoglobulin.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for removing \u03b1-amylase from an oral fluid sample in order to minimize or prevent the screening effects caused by said \u03b1-amylase in a laboratory assay of said oral fluid sample, said method comprising:
contacting said oral fluid sample with starch under conditions enabling binding between the \u03b1-amylase and said starch, at a temperature of 15-35\xb0 C., wherein said starch is not bound to resin and is not chemically bound to another substrate;
separating the starch-amylase bound complexes from the free components, thereby removing the bound \u03b1-amylase;
wherein said contacting and said separating are achieved by means of passing said oral fluid through a filtering device loaded with said starch, wherein said starch is immobilized, applied or disposed on or above a filter that is a sheet of porous media;
collecting the non-bound components in a filtrate having a decreased amount of \u03b1-amylase, thereby obtaining a fluid with a reduced \u03b1-amylase concentration; and
using said fluid with reduced \u03b1-amylase concentration for testing of agents, bio-markers or hormones present in the fluid.
2. The method according to claim 1, wherein the filter is a filter paper.
3. A method according to claim 1, wherein the filtering device through which the sample is passed is a syringe comprising a syringe barrel having an amylase filter occupying a cross-sectional section thereof, wherein the sample is introduced into the syringe barrel and thereafter a syringe plunger is also inserted thereinto and advanced thereinside by means of plunger shaft, such that the sample is forced to pass through said amylase filter.
4. A method according to claim 1, wherein the layer of starch is sandwiched between two porous sheets.
5. A method according to claim 3, wherein the starch is potato starch.
6. A method according to claim 1, wherein said passing of said oral fluid is at a rate of 0.5-2 mlmin.