1. A method for producing helper-dependent adenoviral vectors, said method comprising:
a) providing;
i) an isolated helper-dependent adenoviral DNA sequence comprising an origin of replication linked to a replication-promoting agent,
ii) a helper viral DNA sequence, and
iii) target cells; and
b) transfecting said target cells with said helper-dependent adenoviral DNA sequence and with said helper viral DNA sequence under conditions such that helper-dependent adenoviral vectors are produced.
2. The method of claim 1, wherein said replication-promoting agent is selected from Ad2 preterminal protein, Ad2 terminal protein, Ad5 preterminal protein, and Ad5 terminal protein.
3. The method of claim 1, wherein said replication-promoting agent comprises adenoviral terminal protein.
4. The method of claim 1, wherein said helper-dependent adenoviral DNA sequence comprises a heterologous gene sequence.
5. The method of claim 1, wherein said helper viral DNA sequence comprises helper adenoviral DNA sequence.
6. A method for producing helper-dependent adenoviral vectors, said method comprising:
a) providing;
i) an isolated helper-dependent adenoviral DNA sequence comprising a first origin of replication and a second origin of replication, wherein each of the first and the second origin of replication is linked to a replication-promoting agent,
ii) a helper viral DNA sequence, and
iii) target cells; and
b) transfecting said target cells with said helper-dependent adenoviral DNA sequence and with said helper viral DNA sequence under conditions such that helper-dependent adenoviral vectors are produced.
7. The method of claim 6, wherein said replication-promoting agent is selected from Ad2 preterminal protein, Ad2 terminal protein, Ad5 preterminal protein, and Ad5 terminal protein.
8. The method of claim 6, wherein said replication-promoting agent comprises adenoviral terminal protein.
9. The method of claim 6, wherein said helper-dependent adenoviral DNA sequence comprises a heterologous gene sequence.
10. The method of claim 6, wherein said helper viral DNA sequence comprises helper adenoviral DNA sequence.
11. A host cell comprising an isolated helper-dependent adenoviral DNA sequence comprising a first origin of replication and a second origin of replication, wherein each of the first and the second origin of replication is linked to a replication-promoting agent.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method comprising:
receiving at a server a broadcasted a request for an Internet Protocol (IP) address from a client computer;
upon the server determining that the request for the IP address is from a client computer whose hardware identifier is unknown to the server, directing the client computer to a deployment server; and
downloading a pre-determined set of software from the deployment server to the client computer.
2. The method of claim 1, wherein the directing of the client computer to the deployment server is via an initial direction of the client computer to a domain name server, the domain name server then directing the client computer to the deployment server.
3. The method of claim 1, wherein the pre-determined set of software includes Operating System (OS) configurations.
4. The method of claim 1, wherein the pre-determined set of software includes application software.
5. A computer program product, residing on a computer usable medium, for providing software to a client computer, the computer program product comprising:
program code for receiving at a server a broadcasted a request for an Internet Protocol (IP) address from a client computer;
program code for, upon the server determining that the request for the IP address is from a client computer whose hardware identifier is unknown to the server, directing the client computer to a deployment server; and
program code for downloading a pre-determined set of software from the deployment server to the client computer.
6. The computer program product of claim 5, wherein the directing of the client computer to the deployment server is via an initial direction of the client computer to a domain name server, the domain name server then directing the client computer to the deployment server.
7. The computer program product of claim 5, wherein the pre-determined set of software includes Operating System (OS) configurations.
8. The computer program product of claim 5, wherein the pre-determined set of software includes application software.
9. A system comprising:
a client computer;
a Dynamic Host Configuration Protocol (DHCP) server in communication with the client computer, the DHCP server containing an Internet Protocol (IP) address of only one domain server, the domain server containing an IP address of only one deployment server; and
a deployment server containing the IP address of the only one deployment server stored in the domain server, the deployment server containing a list of software that is authorized to be downloaded to the client computer only upon an authorized user logging into the deployment server from the client computer.
10. The system of claim 9, wherein the list of software includes a link to another website for downloading the software.
11. The system of claim 9, wherein software corresponding with the list of software that is authorized to be downloaded to the client computer is stored in the deployment server.
12. The system of claim 9, wherein the list of software includes application software.
13. The system of claim 9, wherein the list of software includes OS settings.