1. A method for isolating a protein, comprising,
a) suspending chromatography particles in at least one chamber in a centrifugal force field wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the particles, wherein the forces substantially immobilize the particles by the summation of the vector forces acting on the particles, and forming a chromatography bed, wherein each particle is independently suspended without the need for support structure in relation to every other particle to allow for efficient exposure to all surfaces of the particle and the density and shape of the bed may be changed by changing the force parameter on the particles;
b) adding a heterogeneous liquid comprising a protein,
c) separating one or more portions of the heterogeneous liquid by the actions of the chromatography bed; and
d) retaining a separated portion comprising the protein.
2. The method of claim 1, wherein the actions of the chromatography bed separate the protein from the heterogeneous liquid.
3. The method of claim 1, wherein the chromatography particles are adsorbent particles.
4. The method of claim 3, wherein the actions of the chromatography bed separate the heterogeneous liquid by adsorbing the protein to the adsorbent particles.
5. The method of claim 3, wherein the actions of the chromatography bed separate the heterogeneous liquid by allowing materials that are not the protein to pass through the chromatography bed.
6. The method of claim 3, further comprising,
eluting the retained portion comprising the protein from the adsorbent particles; and
collecting the eluted protein.
7. The method of claim 3, wherein the chromatography particles are immunoadsorbent particles.
8. The method of claim 1, wherein the chromatography particles comprise, an adsorbent material; a resin; an agarose particle; a silica particle, an ion exchange particle; a gel; a bead, plastics, glass, methacrylate; anionic particles, cationic particles, polar particles, nonpolar particles, hydrophobic particles, hydrophilic particles, ligand exchange particles, ion-pairing particles, size exclusion particles or affinity chromatography particles.
9. The method of claim 1, wherein the heterogeneous liquid comprises a feed stock, tissue extract, chemical reaction mixture, stock broth, bacterial homogenate, bacterial lysate, E. coli inclusion bodies, products secreted from bacteria, yeast, insect, animal, or plant cells, or tissue homogenates of bacteria, yeast, insect, animal, or plant cells, yeast cell homogenates, cellular homogenates, hybridoma fermentation liquids, myeloma cell culture, animal cell culture liquids, milk, animal tissue extracts, plant tissue extracts, unknown source materials, and culture supernatant from a continuous fluidized bed bioreactor.
10. A method for performing chromatography, comprising,
a) containing chromatography particles in at least one chamber in a centrifugal force field, the chamber having an inlet and an outlet;
b) introducing a heterogeneous liquid to the chamber through the inlet in a liquid stream, wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the particles, wherein the forces substantially immobilize the particles by the summation of the vector forces acting on the particles, wherein each particle is independently suspended without the need for support structure in relation to every other particle to allow for efficient exposure to all surfaces of the particle and the density and shape of the bed may be changed by changing the force parameter on the particles;
c) collecting the liquid passing through the chamber and the particles, and
d) isolating a protein from either the liquid collected or from the chromatographic particles.
11. The method of claim 10, further comprising,
a) providing a second chamber; and
b) containing chromatography particles in the second chamber in a centrifugal force field, the chamber having an inlet and an outlet;
c) introducing the collected liquid to the chamber through the inlet in a liquid stream, wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the particles, wherein the forces substantially immobilize the particles by the summation of the vector forces acting on the particles;
d) collecting the liquid passing through the second chamber and the particles,
e) isolating a protein from either the liquid collected or from the chromatographic particles.
12. The method of claim 10, further comprising,
a) providing a second chamber; and
b) containing chromatography particles in the second chamber in a centrifugal force field, the chamber having an inlet and an outlet;
c) introducing the heterogeneous liquid to the chamber through the inlet in a liquid stream, wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the particles, wherein the forces substantially immobilize the particles by the summation of the vector forces acting on the particles;
d) collecting the liquid passing through the second chamber and the particles,
e) isolating a protein from either the liquid collected or from the chromatographic particles.
13. The method of claim 10, further comprising,
a) introducing the collected liquid to the chamber through the inlet in a liquid stream,
b) collecting the liquid passing through the second chamber and the particles; and
c) repeating the introducing and collecting steps until a predetermined amount of desired product is collected.
14. The method of claim 10, further comprising, changing at least one force in at least one chamber to affect either the density or shape of the chromatographic material.
15. The method of claim 10, wherein containing a chromatographic material in at least one chamber comprises containing the chromatographic material in a plurality of chambers.
16. The method of claim 10, wherein the chromatography particles comprise, an adsorbent material; a resin; an agarose particle; a silica particle, an ion exchange particle; a gel; a bead, plastics, glass, methacrylate; anionic particles, cationic particles, polar particles, nonpolar particles, hydrophobic particles, hydrophilic particles, ligand exchange particles, ion-pairing particles, size exclusion particles or affinity chromatography particles.
17. The method of claim 10, wherein the heterogeneous liquid comprises a purified feed stock, tissue extract, stock broth, bacterial homogenate, bacterial lysate, E. coli inclusion bodies, products secreted from bacteria, yeast, insect, animal, or plant cells, or tissue homogenates of bacteria, yeast, insect, animal, or plant cells, yeast cell homogenates, cellular homogenates, whole hybridoma fermentation liquid, myeloma cell culture, whole animal cell culture liquid, milk, animal tissue extracts, plant tissue extracts, unknown source materials, chemical reaction mixtures, and culture supernatant from a continuous fluidized bed bioreactor.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A combination exercise and massage device comprising:
(a) a lower frame member having a bed assembly and a weight enclosure attached thereto;
(b) said bed assembly having a continuous belt, a plurality of massage rollers and a translating frame assembly including a headrest and shoulder support units;
(c) said weight enclosure including a plurality of weights attached to said translating frame assembly via a weight cable; and
(d) wherein said bed assembly is pivotally mounted to said frame member and includes a locking mechanism such that said bed assembly is capable of tilting to adjust the desired angle, and locking into place in that desired angle for operation;
whereby a user lies on said bed assembly with head on said headrest, shoulders on said shoulder supports and feet against said weight enclosure, and bends then extends the legs to exercise and simultaneously obtain a back massage.
2. The combination exercise and massage device according to claim 1, wherein said translating frame assembly including a headrest and shoulder support units further includes an adjustment bar and locking mechanism such that said translating, frame assembly is user adjustable for the size and height of different users.
3. The combination exercise and massage device according to claim 1, wherein said plurality of massage rollers further include a keyed roller shaft and a variable replaceable outer sleeve.
4. The combination exercise and massage device according to claim 3, wherein said variable replaceable outer sleeves include numerous varied massage elements, and further wherein said massage elements are constructed with materials of varying softness or hardness such that a user may combine massage elements to obtain a desired massage effect.
5. The combination exercise and massage device according to claim 1, wherein said weight enclosure includes handles and rubberized or non-skid foot mats.
6. The combination exercise and massage device according to claim 1, wherein said weight enclosure including a plurality of weights attached to said translating frame assembly via a weight cable, further includes a weight adjustment pin whereby said plurality of weights is user adjustable for a desired level of exercise and massage effect.
7. The combination exercise and massage device according to claim 1, wherein said bed assembly having a continuous belt further includes one or more plain rollers in communication with a belt tightening apparatus for belt tensioning.
8. The combination exercise and massage device according to claim 1, wherein said translating frame assembly further includes padded side members.
9. The combination exercise and massage device according to claim 1, wherein said a translating frame assembly further includes optional massage elements located in the headrest and shoulder supports.
10. The combination exercise and massage device according to claim 9, wherein said optional massage elements located in the headrest and shoulder supports are user adjustable for size and softness or hardness.
11. A method for making a combination exercise and massage device, comprising the steps of:
(a) providing a lower frame member having a bed assembly and a weight enclosure attached thereto;
(b) providing said bed assembly having a continuous belt, a plurality of massage rollers and a translating frame assembly including a headrest and shoulder support units;
(c) providing said weight enclosure including a plurality of weights attached to said translating frame assembly via a weight cable;
(d) pivotally mounted said bed assembly to said frame member and providing a locking mechanism integrated within said bed assembly;
(e) tilting said bed assembly to adjust the desired angle; and
(f) locking said bed assembly into place in that desired angle for operation;
whereby a user lies on said bed assembly with head on said headrest, shoulders on said shoulder supports and feet against said weight enclosure, and bends then extends the legs to exercise and simultaneously obtain a back massage.
12. The method of making a combination exercise and massage device according to claim 11, wherein said step of providing a bed assembly having a translating frame assembly, further includes the step of providing a bed assembly having a translating frame assembly wherein said translating frame assembly includes an adjustment bar and locking mechanism such that said translating frame assembly is user adjustable for the size and height of different users.
13. The method of making a combination exercise and massage device according to claim 11, wherein said step of providing a plurality of massage rollers further includes the step of providing a plurality of massage rollers wherein said plurality of massage rollers further include a keyed roller shaft and a variable replaceable outer sleeve.
14. The method of making a combination exercise and massage device according to claim 13, wherein said step of providing a variable replaceable outer sleeve includes the step of providing a variable replaceable outer sleeve wherein said variable replaceable outer sleeves include numerous varied massage elements, and further wherein said massage elements are constructed with materials of varying softness or hardness such that a user may combine massage elements to obtain a desired massage effect.
15. The method of making a combination exercise and massage device according to claim wherein said step of providing a weight enclosure further includes the step of providing a weight enclosure wherein said weight enclosure includes handles and rubberized or non-skid foot mats.
16. The method of making a combination exercise and massage device according to claim 1 wherein said step of providing a weight enclosure including a plurality of weights attached to said translating frame assembly via a weight cable further includes the step of providing a weight enclosure including a plurality of weights attached to said translating frame assembly via a weight cable wherein said weight enclosure including a plurality of weights attached to said translating frame assembly via a weight cable, further includes a weight adjustment pin whereby said plurality of weights is user adjustable for a desired level of exercise and massage effect.
17. The method of making a combination exercise and massage device according to claim 1 wherein said step of providing a bed assembly having a continuous belt further includes the step of providing a bed assembly having a continuous belt wherein said bed assembly having a continuous belt further includes one or more plain rollers in communication with a belt tightening apparatus for belt tensioning.
18. The method of making a combination exercise and massage device according to claim 1 wherein said step of providing a bed assembly having a translating frame assembly further includes providing a bed assembly having a translating frame assembly wherein said translating frame assembly further includes padded side members.
19. The method of making a combination exercise and massage device according to claim 11, wherein said step of providing a bed assembly having a translating frame assembly further includes providing a bed assembly having a translating frame assembly wherein said a translating frame assembly further includes optional massage elements located in the headrest and shoulder supports.
20. The method of making a combination exercise and massage device according to claim 19, wherein said step of providing a translating frame assembly including optional massage elements located in the headrest and shoulder supports further includes the step of providing a translating frame assembly including optional massage elements wherein said optional massage elements located in the headrest and shoulder supports are user adjustable for size and softness or hardness.