1. A water-soluble polymer represented by chemical formula 1:
wherein, mPEGa and mPEGb are independently monomethoxy polyethylene glycol having molecular weight of 100 to 100,000 Da;
n1 is an integer of 1 to 10;
n2 is an integer of 0 to 10; and Z is \u2014OH, \u2014COOH, \u2014O\u2014C(O)\u2014O\u2014Cl,
2. The water-soluble polymer of claim 1, wherein Z is
3. The water-soluble polymer of claim 1, wherein the polymer is a compound represented by chemical formula 2a:
4. The water-soluble polymer of claim 1, wherein the polymer is a compound represented by chemical formula 3a:
5. The water-soluble polymer of claim 1, wherein mPEGa and mPEGb are the same or different each other, and have a molecular weight of 10,000 Da to 40,000 Da.
6. The water-soluble polymer of claim 1, wherein the polymer further comprises protein, enzyme, nucleotide, lipid, liposome, solid surface, or particle including a functional group being capable of reacting with Z.
7. The water-soluble polymer of claim 6, wherein the polymer is an oligonucleotide aptamer in length of 30 to 45 nucleotides.
8. A method of preparing a compound represented by chemical formula 2a comprising the steps of:
Forming two cyano moieties at a bis-amine group of glycine with cyanoethylation;
Reducing the cyano moieties to amine groups;
Reacting each amine group with monomethoxy polyethylene glycol (mPEG) succinimidyl carbonate to form urethane bond; and
Obtaining the compound of chemical formula 2a by reacting the resultant compound with N-hydroxysuccinimide (NHS):
9. A method of preparing a compound represented by chemical formula 3a comprising the steps of:
Forming two cyano moieties at a bis-amine group of ethanolamine with cyanoethylation;
Reducing the cyano moieties into amine groups;
Reacting each amine group with monomethoxy polyethylene glycol (mPEG) succinimidyl carbonate to form urethane; and
Substituting terminal alcohol group with acid chloride group by reacting with triphosphozine;
Obtaining the compound of chemical formula 3a by reacting the resultant compound with N-hydroxysuccinimide (NHS):
10. A method of preparing a branch-type PEGylated aptamer comprising the steps of:
introducing two phosphoramidite derivatives at 5\u2032-terminus of aptamer to produce a compound of chemical formula 4; and
linking monomethoxy polyethylene glycol (mPEG) having a molecular weight of 10,000 to 20,000 Da to each terminal amine group of the compound of chemical formula 4 to obtain the branch-type PEGylated aptamer represented by chemical formula 5:
In chemical formula 5, x is an integer of 170 to 350.
11. The method of preparing a branch-type PEGylated aptamer according to claim 10, wherein the method is performed by coupling symmetric doubler phosphoramidite to 5\u2032-terminus of an oligonucleotide aptamer synthesized using solid phase polymerization, introducing a terminal amine group of the linked doubler phosphoramidite, and combining the PEG with the terminal amine group.
12. A hyaluronic acid (HA)-aptamer conjugate prepared by linking an aptamer with HA derivative of which a terminal carboxyl group is reacted with adipic acid dihydrazide (ADH), tris(2-aminoethyl)amine (TREN), 2-aminoethyl methacrylate, or N-(3-Aminopropyl) methacrylamide hydrochloride (APMAm).
13. The hyaluronic acid-aptamer conjugate according to claim 12, wherein the HA derivative is selected from the group consisting of the compounds of chemical formulae:
Wherein x and y are each an integer equal to or more than 1, and x+y is 50 to 10,000.
14. The hyaluronic acid-aptamer conjugate according to claim 13, wherein the HA derivative is prepared by reacting ADH or TREN to a carboxyl group of hyaluronic acid in a mixed solvent of water and an organic solvent.
15. The hyaluronic acid-aptamer conjugate according to claim 14, wherein the organic solvent of the mixed solvent ranges from 1 to 90 mol %.
16. The hyaluronic acid-aptamer conjugate according to claim 14, wherein the organic solvent is ethanol.
17. The hyaluronic acid-aptamer conjugate according to claim 13, wherein the hyaluronic acid-aptamer conjugate is prepared by reacting HA-ADH or HA-TREN with the aptamer activated by N-hydroxysuccinimide (NHS).
18. The hyaluronic acid-aptamer conjugate according to claim 13, wherein the hyaluronic acid-aptamer conjugate is prepared by reacting HA-AEMA or HA-APMAm with the aptamer substituted with thiol group at 5\u2032-terminus.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An optical information reproducing apparatus that reproduces information with the use of holography, comprising:
an image acquisition unit that acquires two-dimensional data from a hologram disc;
a maximum likelihood path determination unit that outputs a maximum likelihood path which is a maximum likelihood decoded result of n-th pixel of the two-dimensional data;
a competitive path determination unit that outputs a competitive path which is a decoded result different from the maximum likelihood path of the n-th pixel;
a likelihood calculation unit that outputs a likelihood which is a difference between the maximum likelihood path of the n-th pixel and the competitive path of the n-th pixel;
an inter-signal distance calculation unit that outputs an inter-signal distance which is a distance between a maximum likelihood waveform generated according to the decoded result of the maximum likelihood path of the n-th pixel, and a competitive waveform generated according to the decoded result of the competitive path of the n-th pixel;
a normalized likelihood calculation unit that outputs a normalized likelihood obtained by normalizing the likelihood of the n-th pixel by the inter-signal distance of the n-th pixel;
a reliability output unit that outputs a reliability on the basis of the likelihood candidate; and
an error correction unit that conducts an error correction of data on the basis of the output of the reliability output unit.
2. The optical information reproducing apparatus according to claim 1, further comprising:
a likelihood updating unit that compares likelihood candidates of pixels different in the decoded result of the maximum likelihood path and the decoded result of the competitive path with the normalized likelihood, and sets the normalized likelihood as the likelihood candidate if the normalized likelihood is smaller,
wherein the reliability output unit outputs a reliability on the basis of the likelihood candidate.
3. The optical information reproducing apparatus according to claim 1, further comprising:
a likelihood updating unit that compares likelihood candidates of pixels different in the decoded result of the maximum likelihood path and the decoded result of the competitive path with the likelihood, and sets the likelihood as the likelihood candidate if the likelihood is smaller,
wherein the normalized likelihood calculation unit outputs a normalized likelihood candidate obtained by normalizing the likelihood candidate by the inter-signal distance, and
the reliability output unit outputs a reliability on the basis of the normalized likelihood candidate.
4. An optical information reproducing method of reproducing information with the use of holography, comprising:
acquiring two-dimensional data from a hologram disc;
a maximum likelihood path determination step of outputting a maximum likelihood path which is a maximum likelihood decoded result of n-th pixel of the two-dimensional data;
a competitive path determination step of outputting a competitive path which is a decoded result different from the maximum likelihood path of the n-th pixel;
a likelihood calculation step of outputting a likelihood which is a difference between the maximum likelihood path of the n-th pixel and the competitive path of the n-th pixel;
an inter-signal distance calculation step of outputting an inter-signal distance which is a distance between a maximum likelihood waveform generated according to the decoded result of the maximum likelihood path of the n-th pixel, and a competitive waveform generated according to the decoded result of the competitive path of the n-th pixel;
a normalized likelihood calculation step of outputting a normalized likelihood obtained by normalizing the likelihood of the n-th pixel by the inter-signal distance of the n-th pixel;
a reliability output step of outputting a reliability on the basis of the likelihood candidate; and
an error correction step of conducting an error correction of data on the basis of output of the reliability output step.
5. The optical information reproducing method according to claim 4, further comprising:
a likelihood updating step of comparing likelihood candidates of pixels different in the decoded result of the maximum likelihood path and the decoded result of the competitive path with the normalized likelihood, and setting the normalized likelihood as the likelihood candidate if the normalized likelihood is smaller,
wherein a reliability is set on the basis of the likelihood candidate in the reliability output step.
6. The optical information reproducing method according to claim 4, further comprising:
a likelihood updating step of comparing likelihood candidates of pixels different in the decoded result of the maximum likelihood path and the decoded result of the competitive path with the likelihood, and setting the likelihood as the likelihood candidate if the likelihood is smaller,
wherein a normalized likelihood candidate obtained by normalizing the likelihood candidate by the inter-signal distance is output in the normalized likelihood calculation step; and
a reliability is set on the basis of the normalized likelihood candidate in the reliability output step.