1. A method for controlling a printing device, which comprises the steps of:
A) heating up a primary printing component of the printing device such that at least the surface area of this component has a temperature suitable to perform a first printing job,
B) performing said first printing job, and
C) heating the mass of the primary printing component substantially to it’s surface temperature before a subsequent printing job is performed.
2. The method according to claim 1, wherein during step C) at least a part of the mass of a secondary printing component of the printing device is also heated up substantially to it’s respective surface area temperature.
3. The method according to claim 2, wherein during step C) the substantially entire mass of the secondary printing component is heated up substantially to it’s surface area temperature.
4. The method according to claim 1, wherein step B) and step C) are performed simultaneously for at least a period of time.
5. The method according to claim 1, wherein step A) and step C) are performed simultaneously for at least a period of time.
6. The method according to claim 1 further comprising step D) of performing at least one subsequent printing job after step C) has been completed.
7. The method according to claim 6, which further comprises step E) of heating up the printing device after a subsequent printing job has been performed.
8. The method according to claim 7, which further comprises step F) of allowing the printing device to cool down after completion of all printing jobs.
9. A printing device, comprising:
a primary printing component for performing a printing job, and
a controller for performing the method as claimed in claim 1.
10. The printing device according to claim 9, further comprising a secondary printing component for supporting the primary printing component.
11. The printing device according to claim 10, wherein the printing device further comprises thermal insulation for thermally insulating the primary printing component andor the secondary printing component.
12. A printing device which comprises:
a roller rotatable about its axes, said roller adapted to support a substrate,
a primary printer component operatively associated with said roller for scanning said substrate,
a secondary printing component operatively communicating with the primary printing component for dispensing ink pellets to the primary printing component,
heating means for heating the primary printing component and the secondary printing component, and
controller means for controlling the primary printing component, the secondary printing component and the heating means.
13. The printing device of claim 1, wherein the primary printing component comprises a plurality of printheads.
14. The printing device of claim 1, wherein the primary printer component, the secondary printing component and the heating means are provided with an insulation means.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A composition comprising:
an in vitro culture of cells, said cells comprising a nanogel comprising an active agent and a lytic agent, wherein said lytic agent is provided in an amount sufficient to cause lysis of said stem cells at a predetermined time.
2. The composition of claim 1, wherein said cells are stem cells.
3. The composition of claim 2, wherein said stem cells are selected from the group consisting of pluripotent stem cells and multipotent stem cells.
4. The composition of claim 2, wherein said stem cells are selected from the group consisting of embryonic stem cells and adult stem cells.
5. The composition of claim 2, wherein said stem cells are umbilical cord matrix stem cells.
6. The composition of claim 1, wherein said cells are immune system cells.
7. The composition of claim 6, wherein said immune system cells are selected from the group consisting of leukocytes and lymphocytes.
8. The composition of claim 7, wherein said leukocytes are selected from the neutrophils, macrophages, dendritic cells, mast cells, eosinophils, basophils, monocytes and natural killer cells.
9. The composition of claim 7, wherein said lymphocytes are selected from the group consisting of helper T cells, killer T cells, and B cells.
10. The composition of claim 1, wherein said lytic agent is a detergent.
11. The composition of claim 10, wherein said detergent is selected from the group consisting of Triton X-100 and Tween-20.
12. The composition of claim 1, wherein said cells comprise a suicide gene and said lytic agent is a pro-drug that is activated by the gene product of the suicide gene.
13. The composition of claim 12, wherein said suicide gene is thymidine kinase and said pro-drug is ganciclivor.
14. The composition of claim 1, wherein said nanogel comprises a polymer selected from the group consisting of PEG, PEI, PGA, PLGA and PLA and combinations thereof.
15. The composition of claim 1, wherein said nanogel is a PEGPEI nanogel.
16. The composition of claim 15, wherein said PEGPEI nanogel has a methylene proton ratio (CH2O:CH2N) of about 6.0:1 to about 8.0:1.
17. The composition of claim 1, wherein said predetermined time is from about 36 to 96 hours.
18. The composition of claim 1, wherein said active agent is selected from the group consisting of a therapeutic protein, a therapeutic compound, an antibiotic compound, and an antiviral compound.
19. The composition of claim 18, wherein said therapeutic protein is an antimicrobial polypeptide.
20. The composition of claim 18, wherein said therapeutic compound is a chemotherapeutic compound.
21. A nanogel comprising a therapeutic agent and a lytic agent, wherein said lytic agent is provided in an amount sufficient to cause cell lysis at a predetermined time following introduction into a cell.
22. A composition comprising:
an in vitro culture of stem cells, said cells comprising a nanogel comprising an active agent.
23. A process for making a targeted therapeutic cell composition comprising:
providing a culture of cells and a nanogel comprising a therapeutic agent and a lytic agent, wherein said lytic agent is provided in an amount sufficient to cause lysis of said cells at a predetermined time;
loading said nanogel into said cells to provide nanogel-loaded cells.
24. A method for treating a subject comprising:
administering to a subject in need of treatment the composition of claim 1.
25. A non-toxic nanogel composition comprising particles comprising PEI having a size of from about 0.1 to about 200 nm, wherein said particles are non-toxic when introduced into a cell.
26. The non-toxic nanogel composition of claim 25, further comprising a blocking agent present in a sufficient concentration to block amino groups on said PEI so that said PEI is non-toxic to cells.
27. The non-toxic nanogel composition of claim 26, wherein said blocking agent is PEG and said PEG is present in said composition so that said nanogel has a methylene proton ratio (CH2O:CH2N) of about 6.0:1 to about 8.0:1.
28. The non-toxic nanogel composition of claim 25, wherein said nanogel further comprises PEG cross-linked with said PEI and a blocking moiety.
29. The non-toxic nanogel composition of claim 28, wherein aid blocking agent is selected from the group consisting of an alkyl moiety, and alkenyl moiety, an aryl moiety, and acetyl moiety, and rhodamine.
30. The non-toxic nanogel composition of claim 29, wherein said blocking agent is attached to said nanogel via an amino group on said nanogel.
31. The non-toxic nanogel composition of claim 25, wherein said nanogel composition is lyophilized.
32. The non-toxic nanogel composition of claim 25, wherein said nanogel composition further comprises a labeling agent.
33. A composition comprising:
an in vitro culture of immune system cells, said cells comprising a nanogel comprising an active agent.