1. A method for enhancing the differentiation of a Isl1+ primordial progenitor cells into a vasculargenic lineage cell which express Isl1+ and CD31+, the method comprising:
(a) selecting a population of cultured Isl1+ primordial progenitor cells, wherein the Isl1+ primordial progenitor cells express Isl1 but do not express Nkx2.5 or Kdr, and wherein the Isl1+ primordial progenitor cells are obtained from pluripotent stem cells, and
(b) contacting the population of Isl1+ primordial progenitor cells from step (a) with at least one of VEGF or TGF\u03b2 inhibitor.
2. The method of claim 1, wherein the Isl1+ primordial progenitor is a human Isl1+ primordial progenitor.
3. The method of claim 1, wherein the VEGF is selected from the group consisting of: VEGF165, VEGF A, VEGF B, VEGF C, VEGF D, VEGF E, and VEGF F.
4. The method of claim 1, wherein the TGF\u03b2 inhibitor is an ALK5 inhibitor.
5. The method of claim 4, wherein the ALK5 inhibitor is SB431542 or A-83-01.
6. The method of claim 1, wherein the population Isl1+ primordial progenitors are contacted with VEGF or a TGF\u03b2 inhibitor or both for least 2 days.
7. The method of claim 1, wherein the VEGF is used at a concentration of between 25-50 ngml or a concentration of between 2-10 ngml.
8. The method of claim 4, wherein the ALK5 inhibitor is used at a concentration of between 25-50 ngml or a concentration of between 25-50 ngml.
9. The method of claim 1, wherein the pluripotent stem cells are induced pluripotent stem (iPS) cells.
10. The method of claim 1, wherein the plurpotent stem cells are embryonic stem cells.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A portable radio device comprising:
a first enclosure;
a first printed circuit board provided in the first enclosure;
a second enclosure;
an antenna element provided in the second enclosure, and used at a predetermined frequency;
a second printed circuit board provided in the second enclosure, and feeding power to the antenna element;
a feeding section for feeding power to the antenna element from the second circuit board;
a third enclosure interposed between the first enclosure and the second enclosure;
a first hinge section that rotatably joins the first enclosure to the third enclosure around a first shaft center; and
a second hinge section that rotatably joins the second enclosure to the third enclosure around a second shaft center perpendicular to the first shaft center, wherein
the antenna element is disposed along the second shaft center, at a longitudinal end of the second enclosure in a vicinity of the second hinge section,
the power is fed to the antenna element from a lateral end of the second enclosure opposing to an end in a vicinity of the first shaft center,
the first hinge section includes,
a first retaining section that contains a conductive material electrically connected to the first printed circuit board, and is disposed in the first enclosure,
a second retaining section that contains a conductive material, is disposed in the third enclosure and overlaps the antenna element when the second enclosure and the third enclosure are closed around the second shaft center, and
a first hinge shaft that electrically connects the first retaining section to the second retaining section and contains a conductive material, and is disposed in an area between a substantial center of a plane along which the first enclosure and the third enclosure face to each other and an end of a long side of the plane,
at least one of the first retaining section and the second retaining section being disposed along the second shaft center, and
an electrical path length of the first hinge section which is a sum of an electrical path length of a portion, in the first retaining section, protruding from an end of the first circuit board, an electrical path length of the second retaining section, and an electrical path length of the first hinge shaft, corresponds to a value that is not a substantially natural number multiple of \u03bb8, \u03bb indicating a wavelength that corresponds to the frequency at which the antenna element is used.
2. The portable radio device according to claim 1, wherein the value of the electrical path length is less than \u215b of the wavelength \u03bb.
3. The portable radio device of according to claim 1, wherein at least a part of the first hinge section has a shape that is substantially point symmetrical with respect to a shape of the antenna element when the first enclosure is opened through rotation around the first hinge section.