1. A replication defective recombinant adenovirus comprising a heterologous DNA sequence under the control of an expression signal which is inducible by the an Epstein-Barr virus (EBV) antigen, or by a papilloma virus antigen, wherein the EBV antigen is EBNA1 or EBNA2, and wherein the papilloma virus antigen is E6.
2. An The adenovirus according to claim 1, wherein the expression signal is activated by the EBNA1 antigen, or the EBNA2 antigen.
3. An The adenovirus according to claim 2, wherein the expression signal consists of a chimeric promoter comprising a sequence which is activated by the EBNA1 antigen fused upstream of a viral promoter.
4. An The adenovirus according to claim 1 2, lacking regions of its genome which are required for replication in a target cell.
5. An The adenovirus according to claim 4, wherein said adenovirus is a type Ad5 human adenovirus or a type CAV-2 canine adenovirus.
6. An The adenovirus according to claim 1 2, wherein the heterologous DNA sequence comprises a gene which encodes a product toxic in a cell infected by said adenovirus.
7. An The adenovirus according to claim 6, wherein said product renders said cell sensitive to a therapeutic agent.
8. An The adenovirus according to claim 7, wherein the gene is the thymidine kinase gene and the therapeutic agent is ganciclovir or acyclovir.
9. An The adenovirus according to claim 1 2, wherein the heterologous DNA sequence comprises a gene which encodes a product effective to inhibit cell division.
10. An The adenovirus according to claim 9, wherein the gene is selected from the group consisting of tumour suppressor genes, antisense sequences and ribozymes.
11. An The adenovirus according to claim 6, wherein the heterologous DNA sequence comprises a gene whose expression product induces apoptosis of a cell infected by said adenovirus.
12. A composition comprising a the replication defective recombinant adenovirus according to claim 1 2 and an acceptable carrier.
13. A The composition according to claim 12, in injectable form.
14. The adenovirus of claim 4 3, wherein the viral promoter is the terminal protein 1 (TP1) gene promoter.
15. An isolated cell comprising the adenovirus of claim 1 2.
16. The composition of claim 12 comprising from 106 to 1010 pfuml of replication defective recombinant adenoviruses.
17. The adenovirus according to claim 2, wherein the expression signal is the EBNA1 responsive element (EBNA1-RE).
18. The adenovirus according to claim 14, wherein the sequence which is activated by the EBNA1 antigen is the EBNA1 responsive element (EBNA1-RE).
19. The adenovirus according to claim 17, wherein said adenovirus is a type Ad5 human adenovirus or a type CAV-2 canine adenovirus.
20. The adenovirus of claim 2, wherein the expression signal comprises a promoter sequence from an Epstein-Barr virus.
21. The adenovirus according to claim 20, wherein said adenovirus is a type Ad5 human adenovirus or a type CAV-2 canine adenovirus.
22. The adenovirus according to claim 20, wherein the promoter sequence is inducible by the EBNA1 antigen.
23. The adenovirus according to claim 1, wherein the expression signal is inducible by the papilloma virus E6 antigen.
24. The adenovirus according to claim 2, wherein the expression signal comprises a BCR2 viral promoter.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. Testing method for commencing operation of a system that is controlled with a program logic, comprising:
generating a cross-reference list with identifiers used within the program logic and respective locations of use of the identifiers,
selecting module identifiers that are used to identify modules within the program logic and respective locations of use of the module identifiers, and
generating an overview depiction of at least some requests of a selected one of the modules identified by the respective module identifier, for
selecting at least one specific request of the selected module, and for
observing execution of the at least one specific request of the selected module with an editor,
wherein the overview depiction displays a view showing all modules that request the selected module and any modules that request said all modules that request the selected module, within said execution.
2. Testing method as claimed in claim 1, wherein the system is an automation system.
3. Testing method as claimed in claim 1, wherein the cross-reference list is a complete cross-reference list with all the identifiers used within the program logic and the respective locations of use of the identifiers.
4. Testing method as claimed in claim 1, wherein the program logic is implemented in a graphic programming language and is comprised at least largely of modules.
5. Testing method as claimed in claim 4, wherein the graphic programming language comprises ladder diagram or sequential function chart.
6. Testing method as claimed in claim 1, wherein the locations of use of the module identifiers generated in the cross-reference list respectively comprise the module request and a respective return address for the request.
7. Testing method as claimed in claim 6, further comprising:
analyzing the return addresses related to the selected module, and displaying the overview depiction of the requests of the selected module in a hierarchical request tree with a viewer.
8. Testing method as claimed in claim 7, further comprising:
aborting calculation of a request path of the selected module if the return address related to the selected module is listed multiple times.
9. Testing method as claimed in claim 7, wherein the viewer includes a marking tool that marks at least one line in the request tree and thereby uniquely defines at least one request of the selected module.
10. Testing method as claimed in claim 9, further comprising:
displaying the request of the selected module that is uniquely defined by marking the at least one line in the request tree.
11. Testing device for starting up a system controlled with a program logic, comprising:
a filter element selecting module identifiers, which identify modules of the program logic and respective locations of use of the identifiers, from a cross-reference list of all the identifiers used within the program logic and the locations of use of the identifiers within the program logic,
a display device displaying the list of module identifiers to select one or more modules,
a translation unit translating all requests of this module into a hierarchical display, and
a viewer displaying the hierarchical display showing a selected module, all modules that request the selected module, and any modules that request said all modules that request the selected module.
12. Testing device as claimed in claim 11, wherein the system is an automation system.
13. Testing device as claimed in claim 11, wherein the viewer comprises a marking tool marking individual requests in the hierarchical display.
14. Testing device as claimed in claim 11, further comprising an editor observing execution of a module uniquely defined by selecting a specific request of the selected module.
15. A method, comprising:
translating a program logic into machine language and generating a cross reference list containing identifiers within the translated program logic and locations for each of the identifiers;
selecting module identifiers from the cross-reference list to produce a module identifier list containing module identifiers and locations for each of the module identifiers;
selecting one module identifier from the module identifier list as a selected module; and
generating and displaying a chart showing all modules that request the selected module,
wherein the chart further shows any modules that request said all modules that request the selected module.