What is claimed is:
1. An isolated nucleic acid molecule selected from the group consisting of: a) a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, or the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______;
b) a nucleic acid molecule comprising a fragment of at least 120 nucleotides of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, or the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______;
c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______; or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______;
d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______; and
e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, 3, 4, 6, or a complement thereof, under stringent conditions.
2. The isolated nucleic acid molecule of claim 1, which is selected from the group consisting of:
a) a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______; and
b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______.
3. The nucleic acid molecule of claim 1 further comprising vector nucleic acid sequences.
4. The nucleic acid molecule of claim 1 further comprising nucleic acid sequences encoding a heterologous polypeptide.
5. A host cell which contains the nucleic acid molecule of claim 1.
6. The host cell of claim 5 which is a mammalian host cell.
7. A non-human mammalian host cell containing the nucleic acid molecule of claim 1.
8. An isolated polypeptide selected from the group consisting of:
a) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or a complement thereof.
b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, or a complement thereof under stringent conditions; and
c) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______,wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2 or SEQ ID NO:5.
9. The isolated polypeptide of claim 8 comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5.
10. The polypeptide of claim 8 further comprising heterologous amino acid sequences.
11. An antibody which selectively binds to a polypeptide of claim 8.
12. A method for producing a polypeptide selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______;
b) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______; and
c) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Accession Number ______, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, or a complement thereof under stringent conditions;
comprising culturing the host cell of claim 5 under conditions in which the nucleic acid molecule is expressed.
13. A method for detecting the presence of a polypeptide of claim 8 in a sample, comprising:
a) contacting the sample with a compound which selectively binds to a polypeptide of claim 8; and
b) determining whether the compound binds to the polypeptide in the sample.
14. The method of claim 13, wherein the compound which binds to the polypeptide is an antibody.
15. A kit comprising a compound which selectively binds to a polypeptide of claim 8 and instructions for use.
16. A method for detecting the presence of a nucleic acid molecule of claim 1 in a sample, comprising the steps of:
a) contacting the sample with a nucleic acid probe or primer which selectively hybridizes to the nucleic acid molecule; and
b) determining whether the nucleic acid probe or primer binds to a nucleic acid molecule in the sample.
17. The method of claim 16, wherein the sample comprises mRNA molecules and is contacted with a nucleic acid probe.
18. A kit comprising a compound which selectively hybridizes to a nucleic acid molecule of claim 1 and instructions for use.
19. A method for identifying a compound which binds to a polypeptide of claim 8 comprising the steps of:
a) contacting a polypeptide, or a cell expressing a polypeptide of claim 8 with a test compound; and
b) determining whether the polypeptide binds to the test compound.
20. The method of claim 19, wherein the binding of the test compound to the polypeptide is detected by a method selected from the group consisting of:
a) detection of binding by direct detecting of test compoundpolypeptide binding;
b) detection of binding using a competition binding assay;
c) detection of binding using an assay for 67073-mediated signal transduction.
21. A method for modulating the activity of a polypeptide of claim 8 comprising contacting a polypeptide or a cell expressing a polypeptide of claim 8 with a compound which binds to the polypeptide in a sufficient concentration to modulate the activity of the polypeptide.
22. A method for identifying a compound which modulates the activity of a polypeptide of claim 8, comprising:
a) contacting a polypeptide of claim 8 with a test compound; and
b) determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. Method of operating a burner, said burner having a longitudinal axis, said burner comprising at least one first fuel supply conduit (5) with a first group of fuel outlet openings (6), essentially arranged in the direction of a burner longitudinal axis (3), for the introduction of a first premix fuel quantity into a swirl space and one or a plurality of second fuel supply conduits (7) with a second group of fuel outlet openings (8) essentially arranged in the direction of the burner longitudinal axis (3), it being possible to admit fuel to the second fuel supply conduits (7) independently of the first fuel supply conduit (5), in which the supply of the fuel via the first fuel supply conduits (5) is open-chain controlled or closed-loop controlled separately from the supply of the fuel via the second fuel supply conduits (7), characterized in that the same fuel is supplied to the first and second fuel supply conduits (5, 7).
2. Method according to claim 1, characterized in that premix fuel is supplied to the first fuel supply conduit or conduits (5) and the second fuel supply conduit or conduits (7).
3. Method according to claim 1 or 2, characterized in that gaseous fuel is supplied to the first fuel supply conduit or conduits (5) and the second fuel supply conduit or conduits (7).
4. Method according to one of claims 1 to 3, characterized in that the fuel is introduced into the burner in such a way that it is distributed between the first (5) and the second fuel supply conduits (7) as a function of the load.
5. Method according to one of claims 1 to 3, characterized in that the fuel is introduced into the burner in such a way that it is distributed, as a function of the burner airfuel ratio, between the first (5) and second fuel supply conduits (7).
6. Method according to one of claims 1 to 3, characterized in that in a first operating condition, the total fuel quantity is essentially supplied via the first fuel supply conduit or conduits (5) and is introduced into the combustion airflow via the first group of fuel outlet openings (6), and in that, in a further operating condition, at least a part of the total fuel quantity is supplied to the burner via at least one of the second fuel supply conduits (7) with the second group of fuel supply openings (8).
7. Method of operating a burner according to claims 1 to 3 in a heat generator, characterized in that, in a partial load condition of the heat generator, the total fuel is supplied via the first fuel supply conduits (5), and in that, in at least the full-load operation of the heat generator, the fuel is split between the first fuel supply conduits (5) and at least one second fuel supply conduits (7).
8. Burner, consisting essentially of a swirl generator (1) for a combustion airflow (11), a swirl space (2) and means for introducing fuel into the combustion airflow, the swirl generator (1) having combustion air inlet openings (4) for the combustion airflow entering tangentially into the swirl space (2), which means include means for introducing fuel into the combustion airflow of at least one first fuel supply conduit (5) with a first group of fuel outlet openings (6), essentially arranged in the direction of a burner longitudinal axis (3), for a first premix fuel quantity (P1), and the burner has one or a plurality of second fuel supply conduits (7) with a second group of fuel outlet openings (8), essentially arranged in the direction of the burner longitudinal axis (3), for a second fuel quantity (P2), it being possible to admit fuel to these second fuel supply conduits (7) independently of the first fuel supply conduits (5), characterized in that an inner body (9) is arranged in the swirl space (2), the fuel outlet openings (8) of at least one second fuel supply conduit (7) being arranged on the inner body (9) in such a way that they are essentially distributed in the direction of the burner longitudinal axis (3).
9. Burner according to claim 8, characterized in that the inner body (9) is a fuel lance (12) which has, at its combustion-space end, at least one outlet nozzle for liquid fuel (13) andor pilot fuel.
10. Burner according to claim 8 or 9, characterized in that the fuel outlet openings (8), which are arranged in such a way that they are distributed on the inner body (9) in the direction of the burner longitudinal axis (3), are arranged in a partial axial region of the inner body (9) remote from the combustion-space end.
11. Burner according to one of claims 8 to 10, characterized in that the second group of fuel outlet openings (8) is designed for the supply of premix fuel.
12. Burner according to one of claims 8 to 11, characterized in that at least one of the groups of fuel outlet openings is arranged in the region of at least one of the fuel inlet openings (4).
13. Burner according to one of claims 8 to 12, characterized in that a plurality of first fuel supply conduits (5) and a plurality of second fuel supply conduits (7) are provided, a second fuel supply conduit (7) being associated with each of the first fuel supply conduits (5).
14. Burner according to one of claims 8 to 13, characterized in that second fuel supply conduits (7) are arranged immediately adjacent to first fuel supply conduits (5).
15. Burner according to one of claims 8 to 14, characterized in that the fuel inlet openings (4) are tangential inlet slots extending essentially in the direction of the burner longitudinal-axis (3).
16. Burner according to claim 15, characterized in that a first fuel supply conduit (5) with a first group of fuel outlet openings (6) is arranged along each inlet slot.
17. Burner according to claim 15 or 16, characterized in that at least one second fuel supply conduit (7) with a second group of fuel outlet openings (8) is arranged along each inlet slot.
18. Burner according to one of claims 8 to 17, characterized in that the fuel outlet openings (8) of one or a plurality of second fuel supply conduits (7) are arranged at axial positions between the fuel outlet openings (6) of one or a plurality of first fuel supply conduits (5).
19. Burner according to one of claims 8 to 18, characterized in that the fuel outlet openings (6, 8) of all groups are distributed over the whole of the axial extent of the combustion-air inlet openings (4).
20. Burner according to one of claims 8 to 18, characterized in that the fuel outlet openings (6, 8) of at least one of the groups are distributed over the whole of the axial extent of the combustion-air inlet openings (4) and the fuel outlet openings (6, 8) of at least of one further group are distributed over a partial axial region of the combustion-air inlet openings (4).
21. Burner according to one of claims 8 to 18, characterized in that the fuel outlet openings (6, 8) of at least one of the groups are distributed over a first partial axial region of the combustion-air inlet openings (4) and the fuel outlet openings (6, 8) of other groups are distributed over further partial axial regions of the combustion-air inlet openings (4).
22. Burner according to claim 21, characterized in that the partial axial regions do not overlap.
23. Burner according to claim 21, characterized in that at least two of the partial axial regions overlap at least partially.
24. Burner according to one of claims 8 to 23, characterized in that the fuel outlet openings (6, 8) of two or more groups have different flow cross sections.
25. Burner according to one of claims 8 to 24, characterized in that means are provided for the independent control of the premix fuel supply to the first fuel supply conduit or conduits (5) and to the second fuel supply conduit or conduits (7).
26. Burner according to claim 25, characterized in that means for the independent control of the premix fuel supply have a common fuel line, which branches into a first supply line to the first fuel supply conduit or conduits (5) and into a second supply line to the second fuel supply conduit or conduits (7), a valve (15, 16) for setting the fuel flow quantity being arranged in one of the supply lines.
27. Burner according to one of claims 8 to 26, characterized in that fuel can be admitted to a plurality of the second fuel supply conduits (7) independently of one another.