1. A method of producing induced pluripotent stem cells from mammalian non-pluripotent cells, the method comprising, contacting the cells with at least one of: an agent that inhibits H3K9 methylation or promotes H3K9 demethylation; an L-type Ca channel agonist; an activator of the cAMP pathway; DNA methyltransferase (DNMT) inhibitor; a nuclear receptor ligand; a GSK3 inhibitor; a MEK inhibitor; a TGF\u03b2 receptorALK5 inhibitor; a HDAC inhibitor; and an erk inhibitor, thereby producing induced pluripotent stem cells.
2. (canceled)
3. The method of claim 1 further comprising introducing one or more exogenous polypeptides selected from the group consisting of a KIf polypeptide, an Oct polypeptide, a Myc polypeptide, andor a Sox polypeptide into the non-pluripotent cells.
4. (canceled)
5. A method of producing induced pluripotent stem cells from mammalian non-pluripotent cells, the method comprising, a) introducing one or more exogenous polypeptides selected from the group consisting of: an Oct polypeptide, a KIf polypeptide, a Myc polypeptide, and a Sox polypeptide into the non-pluripotent cells; b) contacting the non-pluripotent cells with an agent that inhibits H3K9 methylation or promotes H3K9 demethylation, thereby producing induced pluripotent stem cells.
6. The method of claim 5, wherein step a) comprises:
(i) at least two cycles of contacting the non-pluripotent cells with one or more exogenous polypeptides selected from the group consisting of: a KIf polypeptide, an Oct polypeptide, a Myc polypeptide, and a Sox polypeptide; followed by culturing the cells in the absence of the exogenous polypeptides;
(ii) contacting the non-pluripotent cells with an exogenous Oct polypeptide and an exogenous Sox polypeptide;
(iii) introducing the one or more exogenous polypeptides into the non-pluripotent cells in vivo;
(iv) introducing the one or more exogenous polypeptides into the non-pluripotent cells ex vivo; or
(v) introducing the one or more exogenous polypeptides into the non-pluripotent cells in vitro.
7.-11. (canceled)
12. The method of claim 5, wherein the non-pluripotent cells are somatic cells.
13. The method of claim 5, wherein the non-pluripotent cells are progenitor cells.
14.-16. (canceled)
17. The method of claim 5, further comprising, c) selecting cells that exhibit pluripotent stem cell characteristics.
18. The method of claim 5, wherein the induced pluripotent stem cells are differentiated into a desired cell type.
19. The method of claim 18, wherein the desired cell type is introduced into the animal.
20. The method of claim 19, wherein the animal is a human.
21.-22. (canceled)
23. The method of claim 5, wherein the agent that inhibits H3K9 methylation is BIX-01294.
24.-27. (canceled)
28. The method of claim 27, wherein the progenitor cells are neural progenitor cells, skin progenitor cells or hair follicle progenitor cells.
29.-30. (canceled)
31. A method for screening for agents that induce reprogramming or dedifferentiation of non-pluripotent mammalian cells into pluripotent stem cells, the method comprising,
a) introducing at least one of, but not all of, an exogenous Oct polypeptide, an exogenous KIf polypeptide, an exogenous Myc polypeptide, and an exogenous Sox polypeptide into the non-pluripotent cells;
b) contacting the non-pluripotent cells to a library of different agents;
c) screening the contacted cells for pluripotent stem cell characteristics; and;
d) correlating the development of stem cell characteristics with a particular agent from the library, thereby identifying an agent that stimulates dedifferentiation of cells into pluripotent stem cells.
32.-40. (canceled)
41. The method of claim 31, wherein the Oct polypeptide is Oct4, the KIf polypeptide is KIf 4, the Myc polypeptide is c-Myc, and the Sox polypeptide is Sox2.
42. The method of claim 13, wherein step (b) comprises contacting cells with a MAPKERK kinase (MEK) inhibitor such that growth of non-pluripotent cells is inhibited and growth of pluripotent stem cells is promoted.
43.-45. (canceled)
46. The method of claim 42, wherein the MEK inhibitor is PD0325901.
47. A mixture of non-pluripotent mammalian cells and an agent that inhibits H3K9 methylation or promotes H3K9 demethylation, wherein the cells are in contact with at least one or more of an exogenous Oct polypeptide, an exogenous KIf polypeptide, an exogenous Sox polypeptide, and an exogenous Myc polypeptide.
48. (canceled)
49. The mixture of claim 47, wherein the agent that inhibits H3K9 methylation is BIX-01294.
50.-52. (canceled)
53. The mixture of claim 47, wherein the cells are human cells.
54. (canceled)
55. The mixture of claim 47, wherein the cells comprise progenitor cells.
56. (canceled)
57. The mixture of claim 47, wherein the Oct polypeptide is Oct4, the KIf polypeptide is KIf 4, the Myc polypeptide is c-Myc, and the Sox polypeptide is Sox2.
58.-68. (canceled)
69. A mixture of non-pluripotent mammalian cells and at least one of an agent that inhibits H3K9 methylation or promotes H3K9 demethylation, an L-type Ca channel agonist; an activator of the cAMP pathway; DNA methyltransferase (DNMT) inhibitor; a nuclear receptor ligand; a GSK3 inhibitor; a MEK inhibitor; a TGF\u03b2 receptorALK5 inhibitor; a HDAC inhibitor; and an erk inhibitor.
70. (canceled)
71. The mixture of claim 69, wherein one or more exogenous polypeptides selected from the group consisting of: a KIf polypeptide, an Oct polypeptide, a Myc polypeptide, and a Sox polypeptide have been introduced into the non-pluripotent cells.
72.-74. (canceled)
75. A composition comprising:
(a) at least one of: an agent that inhibits H3K9 methylation; an L-type Ca channel agonist; an activator of the cAMP pathway; DNA methyltransferase (DNMT) inhibitor; a nuclear receptor ligand; a GSK3 inhibitor; a MEK inhibitor; a TGF\u03b2 receptorALK5 inhibitor; a HDAC inhibitor; and an erk inhibitor; and
(b) one or more polypeptides selected from the group consisting of: a KIf polypeptide, an Oct polypeptide, a Myc polypeptide, and a Sox polypeptide.
76. (canceled)
77. (canceled)
78. A kit comprising (i) an agent that inhibits H3K9 methylation and (ii) an agent selected from the group consisting of an L-type Ca channel agonist; an activator of the cAMP pathway; DNA methyltransferase (DNMT) inhibitor; a nuclear receptor ligand; a GSK3 inhibitor; a MEK inhibitor; a TGF\u03b2 receptorALK5 inhibitor; a HDAC inhibitor; and an erk inhibitor.
79. The kit of claim 78, comprising mammalian cells.
80. The kit of claim 78, comprising one or more polypeptides selected from the group consisting of: a KIf polypeptide, an Oct polypeptide, a Myc polypeptide, and a Sox polypeptide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for a command center to communicate with a movable barrier operator via a satellite system comprising the steps of:
connecting a module capable of telemetry communication to said movable barrier operator;
receiving a first signal from said module via said satellite system to said command center;
sending a second signal from said command center to said module via said satellite system;
deriving a control signal from said second signal from said command center; and
transmitting said control signal from said module to said movable barrier operator.
2. The method of claim 1 wherein communication with said movable barrier operator from said command center further comprises the step of monitoring a status of said movable barrier operator.
3. The method of claim 1 wherein communication with said movable barrier operator from said command center further comprises the step of adjusting a parameter of said movable barrier operator.
4. The method of claim 1 wherein communication with said movable barrier operator from said command center further comprises the step of controlling a function of said movable barrier operator.
5. The method of claim 1 wherein communication with said movable barrier operator from said command center further comprises the step of upgrading a program of said movable barrier operator.
6. The method of claim 2 wherein monitoring said status of said movable barrier operator further comprises the steps of:
checking battery power supply levels of said movable barrier operator;
sensing functionality issues arising in said movable barrier operator; and
sensing changes in weight and in voltage of said movable barrier operator.
7. The method of claim 3 wherein adjusting said parameter of said movable barrier operator further comprises the steps of changing said parameter for:
time delay to close said movable barrier;
overlap delay to open said movable barrier; and
obstruction sensor sensitivity of said movable barrier operator.
8. The method of claim 4 wherein controlling said function of said movable barrier operator further comprises the steps of:
opening said movable barrier;
closing said movable barrier;
stopping any movement of said movable barrier; and
simulating different sensor events in said movable barrier operator for troubleshooting purposes.
9. The method of claim 5 wherein upgrading said program of said movable barrier operator further comprises the step of replacing firmware of said program in order to bring said movable barrier operator up-to-date with current firmware.
10. The method as in claim 9 wherein upgrading said program of said movable barrier operator further comprises the step of replacing said program on:
an individual level;
a regional level;
a countrywide level; and
a global level.
11. The method as in claim 9 wherein upgrading said program of said movable barrier operator further comprises the step of replacing said program by:
a type of access control;
by a customer; and
by a distributor.
12. An access control system for communicating with a movable barrier operator utilizing a satellite system, comprising:
a command center for communicating via said satellite system to a module;
wherein said module is configured for telemetry communication with said command center; and
a movable barrier operator to control a movable barrier, said movable barrier operator connected to said module wherein said command center monitors a status of said movable barrier operator when said command center receives a signal sent from said module via said satellite system.
13. The system of claim 12 wherein said monitoring by said command center includes:
checking of battery power supply levels of said movable barrier operator;
sensing a plurality of functions arising in said movable barrier operator; and
sensing changes in weight and in voltage of said movable barrier operator.
14. The system of claim 12 wherein said command center is capable of adjusting a parameter of said movable barrier by sending a task signal to said module via said satellite system, and wherein said module derives a control signal from said task signal sent via said satellite system from said command center.
15. The system of claim 14 wherein said adjusting by said command center includes changing said parameter for:
a time delay to close said movable barrier;
an overlap delay to open said movable barrier; and
obstruction sensor sensitivity of said movable barrier operator.
16. The system of claim 15 wherein said controlling by said command center includes transmitting said task signal for:
opening said movable barrier;
closing said movable barrier;
stopping any movement of said movable barrier; and
simulating different sensor events in said movable barrier operator for troubleshooting purposes.
17. The system of claim 12 wherein said command center is also for upgrading a program of said movable barrier operator when said module derives said control signal from said task signal sent via said satellite system from said command center.
18. The system of claim 17 wherein said upgrading by said command center includes replacement of firmware of said program in order to make said movable barrier operator up-to-date.
19. The system of claim 18 wherein upgrading said program of said movable barrier operator includes replacement of said program from said command center on an individual basis and at multiple locations.
20. The system of claim 19 wherein upgrading said program of said movable barrier operator includes replacement of said program by:
a type of access control;
a customer; and
a distributor.
21. An access control system for simultaneous communication with and between a plurality of movable barrier operators that control a plurality of movable barriers via a satellite comprising:
a first module configured to communicate with a first movable barrier operator and a command center via said satellite;
a second module configured to communicate with a second movable barrier operator and said command center via said satellite;
wherein said command center transmits a task signal to said first and second modules via said satellite system, and said first and second modules receive said task signal and derive a control signal from said task signal; and
wherein said first and second modules transmit said control signal to said plurality of movable barrier operators to perform a task at said plurality of movable barriers.
22. The access control system of claim 21 wherein said task comprises upgrading a firmware installed at said movable barrier operator.
23. The access control system of claim 22 wherein said task comprises adjusting a parameter of said movable barrier operator.
24. An access control system for communicating with a movable barrier operator utilizing a satellite system, comprising:
a command center for communicating via said satellite system to said movable barrier operator; and
a movable barrier operator connected to a movable barrier,
wherein said movable barrier operator is configured for telemetry communication with said command center, and
wherein said command center monitors a status of said movable barrier operator when said command center receives a signal sent from said movable barrier operator via said satellite system.
25. An access control system for communicating with a movable barrier operator utilizing a satellite system, comprising:
a module configured for telemetry communication with a command center;
a movable barrier operator connected to said module;
wherein said command center is for:
receiving a task signal via said satellite system from said module; and
transmitting a control signal derived from said task signal to said module via said satellite system;
wherein said module is for:
sending said first signal via said satellite system to said command center;
deriving a control signal from said second signal from said command center; and
transmitting said control signal to said movable barrier operator; and wherein said command center is also for:
monitoring a status of said movable barrier operator when said command center receives said first signal sent via said satellite system from said module;
adjusting a parameter of said movable barrier operator when said module derives said control signal from said second signal sent via said satellite system from said command center;
controlling a function of said movable barrier operator when said module transmits said control signal to said movable barrier operator; and
upgrading a program of said movable barrier operator when said module derives said control signal from said second signal via said satellite system from said command center; and wherein said command center is also for:
upgrading said program of said movable barrier operator, which includes replacement of said program from said command center on an individual basis and at multiple locations where there are movable barrier operators within a region, a country, and on a global level.