1. An isolated or purified protein with an amino acid sequence at least 95% identical to SEQ ID NO: 1.
2. The amino acid sequence of claim 1, wherein the sequence is SEQ ID NO:1.
3. A crystal formed from an isolated or purified protein with an amino acid sequence consisting of SEQ ID NO:2, which is Form I, Form II or Form III, wherein Form I has space group C2221 and unit cell parameters of a=86.66-90.86, b=100.83-102.94, and c=65.95-66.62 \u212b, and \u03b1=\u03b2=\u03b3=90.0\xb0; wherein Form II has space group P21 and unit cell parameters of a=63.61-64.97, b=65.44-66.78, and c=64.22-65.95 \u212b, and \u03b1=\u03b3=90.0\xb0, \u03b2=112.66-114.73\xb0; and wherein Form III has space group C2 and unit cell parameters of a=102.51, b=89.25, and c=132.61 \u212b, and \u03b1=\u03b3=90.0\xb0, and \u03b2=89.98\xb0.
4. The crystal of claim 3, which is Form I.
5. The crystal of claim 3, which is Form II.
6. The crystal of claim 3, which is Form III.
7. The crystal of claim 4, in complex with a nucleoside monophosphate (NMP) or an endonuclease inhibitor.
8. A method, comprising contacting a compound with the crystal of claim 4 and determining the structure of the crystal in complex with the compound.
9. A crystal formed from an isolated or purified protein with an amino acid sequence consisting of SEQ ID NO:2, which is Form I, Form II or Form III, wherein Form I has space group C2221 and unit cell parameters of a=88.28, b=102.06, and c=66.26 \u212b, and \u03b1=\u03b2=\u03b3=90.0\xb0; wherein Form II has space group P21 and unit cell parameters of a=65.38, b=66.91, and c=66.14 \u212b, and \u03b1=\u03b3=90.0\xb0, \u03b2=115.34\xb0; wherein Form III has space group C2 and unit cell parameters of a=102.51, b=89.25, and c=132.61 \u212b, and \u03b1=\u03b3=90.0\xb0, \u03b2=89.98\xb0, and wherein the parameters can vary by +\u22125%.
10. The crystal of claim 9, which is Form I.
11. The crystal of claim 9, which is Form II.
12. The crystal of claim 9, which is Form III.
13. The crystal of claim 10, in complex with a nucleoside monophosphate (NMP) or an endonuclease inhibitor.
14. A method, comprising contacting a compound with the crystal of claim 10 and determining the structure of the crystal in complex with the compound.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A lock assembly, said assembly comprising:
a first lock portion, said first lock portion having an open end which receives a key;
a second lock portion operatively connected to said first lock portion;
a torsion spring interposed between said first lock portion and said second lock portion and operatively attached to said first lock portion and said second lock portion such that rotation of one of said first and second lock portions to a first position causes said torsion spring to bias the other of said first and second lock portions;
a housing which contains said first and second lock portions and said torsion spring;
apertures in said housing from which balls are selectively biased;
a terminal end of said second lock portion, said terminal end having a flats portion and an engagement portion; and
wherein said engagement portion of said terminal end of said second lock portion biases said balls from said apertures to a locked position of said lock assembly and said flats portion allows said balls to retreat into said apertures to an unlocked position; and
wherein said second lock portion is capable of biased rotational movement relative to and independent of said first lock portion such that said lock assembly can be held in an intermediate position between said locked and unlocked positions in which said balls are urged partially out of said apertures by said rotationally biased engagement portion enabling said lock assembly to be preloaded into a locking device thereby eliminating the need for an installation key.
2. The lock assembly of claim 1, further comprising a split ring locking device, said split ring including:
a curved sidewall having a first end and a second end;
a collar on said first end having a bore which receives said lock assembly, said bore including at least one relatively shallow aperture;
a bushing on said second end with an opening that receives said lock assembly, said bushing opening including at least one relatively deep aperture; and
wherein said lock assembly may pass through said bore into said bushing opening to lock said assembly when said collar and bushing are axially aligned.
3. The lock assembly of claim 2, wherein said balls are biased partially out of said apertures of said housing and into said at least one relatively shallow aperture of said bore of said collar to preload said lock assembly.
4. The lock assembly of claim 3, wherein when said bushing is axially aligned with said collar preloaded with said lock assembly, the assembly may be pushed axially into said bushing thereby disengaging said balls from said at least one relatively shallow aperture and forcing said biased balls into said at least one relatively deep aperture thereby locking said split ring.
5. A barrel lock with a rotating locking mechanism, said barrel lock comprising:
a stem, said stem having a terminal end portion which locks and unlocks said barrel lock;
a cylinder having a first end and a second end, said first end containing a rotating locking mechanism and said second end including a means for rotatably attaching said stem to said cylinder;
a torsion spring interposed between said stem and said cylinder and operatively attached to said stem and said cylinder for rotationally biasing said stem relative to said cylinder when said cylinder is rotated to a preloaded position;
a housing which contains said stem, cylinder, means for rotatably attaching said stem, and said torsion spring;
apertures in said housing from which balls are selectively biased;
said terminal end portion of said stem having a flats portion and an engagement portion;
wherein said engagement portion of said terminal end of said stem biases said balls from said apertures to a locked position of said lock assembly and said flats portion allows said balls to retreat into said apertures to an unlocked position; and
wherein said stem is capable of biased rotational movement relative to and independent of said cylinder such that said barrel lock can be held in an intermediate position between said locked and unlocked positions in which said balls are biased partially out of said apertures enabling said barrel lock to be preloaded into a lock assembly thereby eliminating the need for an installation key.