1. An electrophoresis gel matrix comprising:
at least one chemical reagent being at least one of a denaturing, buffering, disaggregating, reducing, staining or dissolving reagent, wherein:
the matrix contains a volume of dimethylsulfoxide.
2. Matrix of claim 1, wherein the matrix is an electrophoresis gel matrix for conducting proteomics.
3. Matrix of claim 1, wherein the matrix is a Poly-N,N-Dimethylacrylamide (PDMA), Methylcellulose (MC), Hydroxyethylcellulose (HEC), Hydroxypropyl cellulose (HPC), Hydroxypropylmethylcellulose (HPMC), Dextran, linear Polyacrylamide, Poly-N-Acryloylaminoethoxyethanol (PMEE), Polyacryloylaminopropanol (PAAP), Poly-(acryloylaminoethoxy)ethyl-glucopyranoside (PAEG), Polyethylene Glycol (PEG), Polyethyleneoxide (PEO) or Polyvinyl pyrrolidone (PVP) matrix.
4. Matrix of claim 1, wherein the volume of dimethylsulfoxide ranges from 4% to 15%, with respect to the total volume of chemical reagents being comprised in the matrix.
5. Matrix of claim 1, wherein the buffering reagent is a tris-tricine buffer andor the denaturing reagent is sodiumdodecylsulfate andor the dissolving and reducing reagent is a thiol containing compound andor the staining reagent is a fluorescent dye.
6. Matrix according to claim 5, wherein the fluorescent dye is water soluble andor stable in aqueous solutions.
7. The use of dimethylsulfoxide in a matrix according to claim 1, as enhancing reagent for the resolution of proteins.
8. The use of dimethylsulfoxide in matrices for conducting proteomics comprising separation andor identification of proteins.
9. The use according to claim 7, wherein a volume of dimethylsulfoxide is added to the matrix which ranges from 4% to 15%, with respect to the total volume of chemical reagents being comprised in the matrix.
10. The use according to claim 7, wherein sodiumdodecylsulfate is added to the matrix in order to denature the proteins, andor tis-tricine is added in order to buffer the matrix, andor a thiol containing compound is used in order dissolve and reduce proteins, andor a fluorescent dye is added to the matrix in order to stain proteins.
11. The use of a matrix according to claim 1, for being filled into an electrophoresis capillary being part of a microfluidic device for carrying out proteomics.
12. The use of a matrix according to claim 12, for obtaining an enhanced resolution in comparison to a resolution obtained by using a matrix containing less than 2% dimethylsulfoxide with respect to the total volume of reagents.
13. A method for preparing an electrophoresis gel matrix for conducting proteomics according to claim 1 comprising
adding of at least one chemical reagent being at least a denaturing, buffering, disaggregating, reducing, staining or dissolving reagent, and
adding a volume of dimethylsulfoxide to a Poly-N,N-Dimethylacrylamide (PDMA), Methylcellulose (MC), Hydroxyethylcellulose (HEC), Hydroxypropylcellulose (HPC), Hydroxypropylmethylcellulose (HPMC), Dextran, linear Polyacrylamide, PolyN-Acryloylaminoethoxyethanol (PMEE), Polyacryloylaminopropanol (PAAP), Poly-(acryloylaminoethoxy)ethyl-glucopyranoside (PAEG), Polyethylene Glycol (PEG), Polyethyleneoxide (PEO) or Polyvinyl Pyrrolidone (PVP) matrix gel.
14. A method for preparation of an electrophoresis capillary as separation channel comprised in a microfluidic device, wherein an electrophoresis gel matrix according to claim 1 is filled into the capillary preferably under pressure.
15. A method for carrying out separation and identification of proteins, comprising:
introducing a sample containing proteins into a microfluidic device comprising an electrophoresis capillary as separation channel,
moving the sample through the separation channel in order to sieve the proteins according to their size, and
identifying the proteins when leaving the electrophoresis capillary by application of an identification method,
wherein results obtained by the identification method show an enhanced resolution in comparison to results obtained by performing an identical method for carrying out separation and identification using a matrix which contains less than 2% dimethylsulfoxide with respect to the total volume of chemical reagents being comprised in the matrix.
16. Method according to claim 15, wherein the enhancement of the resolution is optically detected.
17. Method according to claim 15, wherein the enhancement of the resolution is measured by fluid conductivity measurements.
18. Method according to claim 15, wherein the identification method is an optical detection technique.
19. Method according to claim 18, wherein the optical detection technique includes laser induced fluorescence.
20. Matrix of claim 1, wherein the volume of dimethylsulfoxide ranges from 5% to 10% with respect to the total volume of chemical reagents being comprised in the matrix.
21. Matrix of claim 1, wherein the volume of dimethylsulfoxide ranges from 6% to 8% with respect to the total volume of chemical reagents being comprised in the matrix.
22. The use according to claim 7, wherein a volume of dimethylsulfoxide is added to the matrix which ranges from 5% to 10% with respect to the total volume of chemical reagents being comprised in the matrix.
23. The use according to claim 7, wherein a volume of dimethylsulfoxide is added to the matrix which ranges from 6% to 8% with respect to the total volume of chemical reagents being comprised in the matrix.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for digital subscriber line signal processing, comprising:
using a symbol in a frame bearing data to be sent;
acquiring a quantity of remaining symbols that do not bear the data in the frame; and
adding information about the quantity of the remaining symbols in the frame, and sending the frame with the information about the quantity of the remaining symbols.
2. The method according to claim 1, wherein when the remaining symbols are sent, signal sending power of the remaining symbols is set to 0.
3. The method according to claim 2, wherein the sending the frame with the information comprises:
generating a downlink timeslot synchronization signal, and sending the frame with the information about the quantity of the remaining symbols, when triggered by the downlink timeslot synchronization signal.
4. The method according to claim 2, further comprising:
setting, when sending the sent frame, signal sending power of all symbols in a pre-assigned symbol set in the frame with the information about the quantity of the remaining symbols to a non-0 value.
5. The method according to claim 1, further comprising: replacing the remaining symbols with idle symbols known by a receiving side.
6. The method according to claim 5, wherein the idle symbols are the same as a last symbol that bears the sent data, or a specific symbol which modulation mode and bit bearer content are pre-arranged by sending and receiving sides.
7. The method according to claim 1, further comprising: replacing a part of the remaining symbols with idle symbols known by a receiving side, and setting signal sending power of the other part of the remaining symbols to 0.
8. An apparatus for digital subscriber line signal processing, comprising:
a bearing module, configured to use a symbol in a frame bearing data to be sent;
a remaining symbol acquiring module, configured to acquire a quantity of remaining symbols that do not bear the data in the frame; and
an adding and sending module, configured to add information about the quantity of the remaining symbols in the frame, and send the frame with the information about the quantity of the remaining symbols.
9. The apparatus according to claim 8, further comprising:
a first power setting module, configured to set, signal sending power of the remaining symbols to 0, when the adding and sending module sends the remaining symbols.
10. The apparatus according to claim 9, further comprising:
a synchronization signal generating module, configured to generate a downlink timeslot synchronization signal, wherein:
the adding and sending module is configured to send, the frame with the information about the quantity of the remaining symbols, when triggered by the downlink timeslot synchronization signal.
11. The apparatus according to claim 9, wherein the first power setting module is further configured to set, signal sending power of all symbols in a pre-assigned symbol set in the sent frame to a non-0 value, when the adding and sending module sends the frame with the information about the quantity of the remaining symbols.
12. The apparatus according to claim 8, further comprising:
a symbol replacing module, configured to replace the remaining symbols with idle symbols known by a receiving side.
13. The apparatus according to claim 12, wherein the idle symbols are the same as a last symbol that bears the sent data or a specific symbol which modulation mode and bit bearer content are pre-arranged by sending and receiving sides.
14. The apparatus according to claim 8, wherein the symbol replacing module is configured to replace a part of the remaining symbols with idle symbols known by a receiving side;
the apparatus further comprises:
a second power setting module, configured to set signal sending power of the other part of the remaining symbols to 0.
15. A system for digital subscriber line signal processing, comprising an xDSL (digital subscriber line) transceiver at a central office and an xDSL transceiver at a customer premises, wherein the xDSL transceiver at a central office is connected to the xDSL transceiver at a customer premises through a twisted pair, and at least one of the xDSL transceiver at the central office and the xDSL transceiver at the customer premises comprises an apparatus for digital subscriber line signal processing;
wherein the apparatus for digital subscriber line signal processing comprises:
a bearing module, configured to use a symbol in a frame bearing data to be sent;
a remaining symbol acquiring module, configured to acquire a quantity of remaining symbols that do not bear the data in the frame; and
an adding and sending module, configured to add information about the quantity of the remaining symbols in the frame, and send the frame with the information about the quantity of the remaining symbols.