1. A method, comprising providing a composition comprising recombinant paramyosin, contacting said composition with a strand separation agent to yield a solution of paramyosin monomers, removing low molecular weight moieties from said solution of monomers, and removing said strand separation agent to yield a product comprising purified full length Schistosomal paramyosin coiled coil dimer, wherein said paramyosin is selected from the group consisting of Schistosoma japonicum 97 (Sj97) and Schistosoma mansoni (Sm97), and wherein said strand separation agent comprises Guanidium Hydrochloride (Gu-HCl) with a concentration of about 7M, urea with a concentration of at least 9M, or an ionic detergent, wherein said ionic detergent comprises at least 0.02% (ww) sodium dodecyl sulfate (SDS), at least 0.2% (ww) N-lauryl sarcosine, or deoxycholic acid.
2. The method of claim 1, wherein said product comprises less than 5% (ww) paramyosin fragments.
3. The method of claim 1, wherein said strand separation agent comprises an ionic detergent.
4. The method of claim 1, wherein said strand separation agent comprises at least 0.05% (ww) sodium dodecyl sulfate.
5. The method of claim 1, wherein said method yields at least 25 mg of purified paramyosin per 10 liters.
6. The method of claim 1, wherein said strand separation agent comprises Gu-HCl with a concentration of about 7M.
7. The method of claim 1, wherein said strand separation agent comprises urea with a concentration of at least 9M.
8. The method of claim 1, wherein said strand separation agent comprises at least 0.02% SDS.
9. The method of claim 1, wherein said strand separation agent comprises N-lauryl sarcosine.
10. The method of claim 1, wherein said strand separation agent comprises deoxycholic acid.
11. A method, comprising providing a composition comprising recombinant paramyosin, contacting said composition with a strand separation agent to yield a solution of paramyosin monomers, removing low molecular weight moieties from said solution of monomers, and removing said strand separation agent to yield a product comprising purified full length Schistosomal paramyosin coiled coil dimer, wherein said paramyosin is selected from the group consisting of Schistosoma japonicum 97 (Sj97) and Schistosoma mansoni (Sm97), and wherein said strand separation agent comprises 8M urea with a temperature of at least 60\xb0 C.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A starter solenoid switch comprising:
a first set of contacts including a first fixed contact and a first movable contact, said first set of contacts being disposed between a battery and a starter motor to make and break an electrical connection therebetween; and
a second set of contacts including a second fixed contact and a second movable contact, said second set of contacts being disposed electrically parallel to said first set of contacts between the battery and the starter motor, said first and said second sets of contacts being so configured that the second movable contact makes contact with the second fixed contact to supply a lighter electric current to the starter motor before the first movable contact makes contact with the. first fixed contact to supply a heavier electric current to the starter motor,
wherein one of the second fixed contact and the second movable contact includes a low-resistance portion at one end thereof and a high-resistance portion that contains more carbon than the low-resistance portion, and wherein the low-resistance and the high-resistance portions are serially disposed along a conducting path of electric current from the battery to the starter motor.
2. The starter solenoid switch as set forth in claim 1, wherein the low-resistance portion of the one of the second fixed contact and the second movable contact has an electric resistivity of 100 \u03bc\u03a9cm or less, and the high-resistance portion of the same has an electric resistivity of 100 times or more higher than that of the low-resistance portion.
3. The starter solenoid switch as set forth in claim 1, further comprising an electrically conductive member that is electrically and mechanically connected to the low-resistance portion of the one of the second fixed contact and the second movable contact.
4. The starter solenoid switch as set forth in claim 3, wherein the electrically conductive member is a twisted wire made of a metal material.
5. The starter solenoid switch as set forth in claim 3, wherein the electrically conductive member is a metal plate.
6. The starter solenoid switch as set forth in claim 1, wherein the one of the second fixed contact and the second movable contact further includes an intermediate portion that is interposed between the low-resistance portion and the high-resistance portion and has a coefficient of thermal expansion between those of the low-resistance and the high-resistance portions.
7. The starter solenoid switch as set forth in claim 1, wherein the second fixed contact includes the low-resistance portion and the high-resistance portion.
8. The starter solenoid switch as set forth in claim 7, wherein the second fixed contact is so arranged that the low-resistance portion is to be connected to an external terminal, which is connected to the battery, in a manner allowing heat transfer from the second fixed contact to the external terminal.
9. The starter solenoid switch as set forth in claim 8, further comprising an electrically conductive member, wherein the second fixed contact is so arranged that the low-resistance portion is to be connected to the external terminal via the electrically conductive member.
10. The starter solenoid switch as set forth in claim 7, further comprising a terminal and an electrically conductive member both of which are integrally formed with the low-resistance portion of the second fixed contact by molding.
11. A starter solenoid switch comprising:
a first set of contacts including a first fixed contact and a first movable contact, said first set of contacts being disposed between a battery and a starter motor to make and break an electrical connection therebetween; and
a second set of contacts including a second fixed contact and a second movable contact, said second set of contacts being disposed electrically parallel to said first set of contacts between the battery and the starter motor, said first and said second sets of contacts being so configured that the second movable contact makes contact with the second fixed contact to supply a lighter electric current to the starter motor before the first movable contact makes contact with the first fixed contact to supply a heavier electric current to the starter motor,
wherein one of the second fixed contact and the second movable contact includes a first low-resistance portion at a first end thereof, a second low-resistance portion at a second end thereof that is opposite to the first end, and a high-resistance portion between the first and the second low-resistance portions which contains more carbon than both the first and the second low-resistance portions, and wherein the first low-resistance portion, the high-resistance portion, and the second low-resistance portion are serially disposed along a conducting path of electric current from the battery to the starter motor.
12. The starter solenoid switch as set forth in claim 1, wherein both the first and the second low-resistance portions of the one of the second fixed contact and the second movable contact has an electric resistivity of 100 \u03bc\u03a9cm or less, and the high-resistance portion of the same has an electric resistivity of 100 times or more higher than those of the first and the second low-resistance portions.
13. The starter solenoid switch as set forth in claim 1, further comprising an electrically conductive member that is electrically and mechanically connected to one of the first and the second low-resistance portions of the one of the second fixed contact and the second movable contact.
14. The starter solenoid switch as set forth in claim 13, wherein the electrically conductive member is a twisted wire made of a metal material.
15. The starter solenoid switch as set forth in claim 13, wherein the electrically conductive member is a metal plate.
16. The starter solenoid switch as set forth in claim 13, wherein the first low-resistance portion of the one of the second fixed contact and the second movable contact is electrically and mechanically connected to the electrically conductive member and the second low-resistance portion of the same faces the other one of the second fixed contact and the second movable contact.
17. The starter solenoid switch as set forth in claim 11, wherein the one of the second fixed contact and the second movable contact further includes a first and a second intermediate portion that are interposed between the first low-resistance portion and the high-resistance portion and between the second low-resistance portion and the high-resistance portion, respectively, and have a coefficient of thermal expansion between those of the first and the second low-resistance portions and the high-resistance portion.
18. The starter solenoid switch as set forth in claim 11, wherein the second fixed contact includes the first low-resistance portion, the high-resistance portion, and the second low-resistance portion.
19. The starter solenoid switch as set forth in claim 18, wherein the second fixed contact is so arranged that one of the first and the second low-resistance portions is to be connected to an external terminal, which is connected to the battery, in a manner allowing heat transfer from the second fixed contact to the external terminal.
20. The starter solenoid switch as set forth in claim 19, further comprising an electrically conductive member, wherein the second fixed contact is so arranged that one of the first and the second low-resistance portions is to be connected to the external terminal via the electrically conductive member.
21. The starter solenoid switch as set forth in claim 18, further comprising a terminal and an electrically conductive member both of which are integrally formed with one of the first and the second low-resistance portions of the second fixed contact by molding.