1. A method for the expression of a coding region of interest in a transformed yeast cell comprising:
a) providing a transformed yeast cell having a recombinant construct, wherein the recombinant construct comprises:
(1) a promoter region of an ALK2 Yarrowia gene; and
(2) a coding region of interest which is expressible in the yeast cell;
wherein the promoter region is operably linked to the coding region of interest; and
b) growing the transformed yeast cell of step (a) under conditions whereby the recombinant construct of step (a) is expressed.
2. The method according to claim 1, wherein the promoter region of the ALK2 Yarrowia gene comprises SEQ ID NO:56.
3. The method according to claim 1, wherein the promoter region of the ALK2 Yarrowia gene comprises SEQ ID NO:6, wherein said promoter optionally comprises at least one modification selected from the group consisting of:
a) a deletion at the 5\u2032-terminus of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 603, 604, 605, 606, 607, 608, 609, 610, 611, 612, 613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, or 634 consecutive nucleotides, wherein the first nucleotide deleted is the adenine nucleotide \u2018A\u2019 at position 1 of SEQ ID NO:6;
b) a deletion at the 3\u2032-terminus of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, or 66 consecutive nucleotides, wherein the first nucleotide deleted is the cytosine \u2018C\u2019 nucleotide at position 942 of SEQ ID NO:6;
c) substitution of a guanine \u2018G\u2019 nucleotide for the cytosine \u2018C\u2019 nucleotide at position 753 of SEQ ID NO:6;
d) substitution of an adenine \u2018A\u2019 nucleotide or a thymine \u2018T\u2019 nucleotide for the cytosine \u2018C\u2019 nucleotide at position 753 of SEQ ID NO:6;
e) substitution of the nucleotide sequence \u2018AA\u2019 for the nucleotide sequence \u2018TT\u2019 at positions 41 and 42 of SEQ ID NO:6;
f) insertion of the nucleotide sequence \u2018TOG\u2019 between positions 109 and 110 of SEQ ID NO:6;
g) insertion of the nucleotide sequence \u2018AAAT\u2019 between position 131 and position 132 of SEQ ID NO:6;
h) substitution of the nucleotide sequence \u2018AAA\u2019 for the nucleotide sequence \u2018TT\u2019 at positions 76 to 77 of SEQ ID NO:6;
i) substitution of the nucleotide sequence \u2018GTTT\u2019 for the nucleotide sequence \u2018AAAA\u2019 at positions 631 to 634 of SEQ ID NO:6;
j) insertion of the nucleotide sequence \u2018TA\u2019 between position 512 and position 513 of SEQ ID NO:6;
k) insertion of the nucleotide sequence \u2018TTTA\u2019 between position 365 and position 366 of SEQ ID NO:6;
l) insertion of the nucleotide sequence \u2018TTAAA\u2019 between position 230 and position 231 of SEQ ID NO:6; and
m) any combination of part a), part b), part c), part d), part e), part f), part g), part h), part i), part j), part k), and part l).
4. The method according to claim 3, wherein the promoter region of the ALK2 Yarrowia gene comprises a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:27, SEQ ID NO:15, SEQ ID NO:47, SEQ ID NO:43, SEQ ID NO:39, SEQ ID NO:35, SEQ ID NO:31, SEQ ID NO:19, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53 and SEQ ID NO:55.
5. The method according to claim 4, wherein the promoter region of the ALK2 Yarrowia gene comprises SEQ ID NO:55, and wherein said promoter region optionally comprises at least one modification selected from the group consisting of:
a) a deletion at the 5\u2032-terminus of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, or 174 consecutive nucleotides, wherein the first nucleotide deleted is the adenine nucleotide \u2018A\u2019 at position 1 of SEQ ID NO:55; and
b) a deletion of part (a) in combination with a substitution of a thymine \u2018T\u2019 nucleotide, adenine \u2018A\u2019 nucleotide or a cytosine \u2018C\u2019 nucleotide for the guanine \u2018G\u2019 nucleotide at position 119 of SEQ ID NO:55.
6. The method according to claim 1, wherein the transformed yeast cell is an oleaginous yeast cell.
7. The method of claim 6, wherein the oleaginous yeast cell is a member of a genus selected from the group consisting of Yarrowia, Candida, Rhodotorula, Rhodosporidium, Cryptococcus, Trichosporon and Lipomyces.
8. The method according to claim 1, wherein the coding region of interest encodes a polypeptide, wherein the polypeptide is selected from the group consisting of: desaturases, elongases, acyltransferases, aminopeptidases, amylases, carbohydrases, carboxypeptidases, catalases, cellulases, chitinases, cutinases, cyclodextrin glycosyltransferases, deoxyribonucleases, esterases, alpha-galactosidases, beta-galactosidases, glucoamylases, alpha-glucosidases, beta-glucanases, beta-glucosidases, invertases, laccases, lipases, mannosidases, mutanases, oxidases, pectinolytic enzymes, peroxidases, phospholipases, phosphatases, phytases, polyphenoloxidases, proteolytic enzymes, ribonucleases, transglutaminases and xylanases.
9. The method according to claim 1, wherein the promoter region of the ALK2 Yarrowia gene further (i) comprises an enhancer region set forth in SEQ ID NO:57, or (ii) is operably linked to said enhancer region.
10. The method according to claim 1, wherein
the coding region encodes at least one omega-3 fatty acid or omega-6 fatty acid biosynthetic pathway enzyme, and
wherein an omega-3 fatty acid or omega-6 fatty acid is produced in step b).
11. The method according to claim 10, wherein the omega-3 fatty acid or omega-6 fatty acid biosynthetic pathway enzyme is selected from the group consisting of desaturases and elongases.
12. An isolated nucleic acid molecule comprising a promoter region of an ALK2 Yarrowia gene as set forth in SEQ ID NO:6, wherein said promoter region optionally comprises at least one modification selected from the group consisting of:
a) a deletion at the 5\u2032-terminus of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 603, 604, 605, 606, 607, 608, 609, 610, 611, 612, 613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, or 634 consecutive nucleotides, wherein the first nucleotide deleted is the adenine nucleotide \u2018A\u2019 at position 1 of SEQ ID NO:6;
b) a deletion at the 3\u2032-terminus of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, or 66 consecutive nucleotides, wherein the first nucleotide deleted is the cytosine \u2018C\u2019 nucleotide at position 942 of SEQ ID NO:6;
c) substitution of a guanine \u2018G\u2019 nucleotide for the cytosine \u2018C\u2019 nucleotide at position 753 of SEQ ID NO:6;
d) substitution of an adenine \u2018A\u2019 nucleotide or a thymine \u2018T\u2019 nucleotide for the cytosine \u2018C\u2019 nucleotide at position 753 of SEQ ID NO:6;
e) substitution of the nucleotide sequence \u2018AA\u2019 for the nucleotide sequence \u2018TT\u2019 at positions 41 and 42 of SEQ ID NO:6;
f) insertion of the nucleotide sequence \u2018TCG\u2019 between positions 109 and 110 of SEQ ID NO:6;
g) insertion of the nucleotide sequence \u2018AAAT\u2019 between position 131 and position 132 of SEQ ID NO:6;
h) substitution of the nucleotide sequence \u2018AAA\u2019 for the nucleotide sequence \u2018TT\u2019 at positions 76 to 77 of SEQ ID NO:6;
i) substitution of the nucleotide sequence \u2018GTTT\u2019 for the nucleotide sequence \u2018AAAA\u2019 at positions 631 to 634 of SEQ ID NO:6;
j) insertion of the nucleotide sequence \u2018TA\u2019 between position 512 and position 513 of SEQ ID NO:6;
k) insertion of the nucleotide sequence \u2018TTTA\u2019 between position 365 and position 366 of SEQ ID NO:6;
l) insertion of the nucleotide sequence \u2018TTAAA\u2019 between position 230 and position 231 of SEQ ID NO:6; and
m) any combination of part a), part b), part c), part d), part e), part f), part g), part h), part i), part j), part k), and part l).
13. The isolated nucleic acid molecule of claim 12, wherein the promoter region comprises a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:27, SEQ ID NO:15, SEQ ID NO:47, SEQ ID NO:43, SEQ ID NO:39, SEQ ID NO:35, SEQ ID NO:31, SEQ ID NO:19, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53 and SEQ ID NO:55.
14. The isolated nucleic acid molecule of claim 12, wherein the promoter region of the ALK2 Yarrowia gene further (i) comprises an enhancer region set forth in SEQ ID NO:57, or (ii) is operably linked to said enhancer region.
15. An isolated nucleic acid molecule comprising a promoter region of an ALK2 Yarrowia gene comprising SEQ ID NO:56.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A display control system comprising:
a display control apparatus including a command data transmission section for transmitting command data; and
a display apparatus for performing display based on command data transmitted from the command data transmission section,
wherein the display apparatus includes:
a command data receiving section for receiving the command data;
a command data interpretation section for applying a mathematical operation to the received command data so as to generate image data; and
a display section for displaying an image in accordance with the image data.
2. A display control system according to claim 1, wherein the command data interpretation section also serves as the command data receiving section.
3. A display control system according to claim 1, wherein the command data interpretation section generates the image data based on data which is received via the command data receiving section.
4. A display control system according to claim 1, wherein the command data transmission section and the command data receiving section are coupled via a transfer cable.
5. A display control system according to claim 1, wherein the command data transmission section and the command data receiving section are coupled via a wireless device.
6. A display control system according to claim 1, wherein the command data contains draw command data.
7. A display control system according to claim 1, wherein the command data contains at least one of error check information and error correction information.
8. A display control system according to claim 1, wherein the command data is encrypted.
9. A display control method for a display control system including a display control apparatus including a command data transmission section for transmitting command data and a display apparatus for performing display based on command data transmitted from the command data transmission section,
wherein the method comprises the steps of:
transferring command data from the display control apparatus to the display apparatus;
applying a mathematical operation to the transferred command data in the display apparatus to generate image data; and
displaying an image in accordance with the image data.
10. A display apparatus for performing display based on command data transmitted from outside of the display apparatus, comprising:
a command data interpretation section for applying a mathematical operation to command data so as to generate image data; and
a display section for displaying an image in accordance with the image data.
11. A display apparatus according to claim 10, wherein the command data interpretation section also serves as the command data receiving section.
12. A display apparatus according to claim 10, wherein the command data interpretation section generates the image data based on data which is received via the command data receiving section.