1. A cytotoxic protein comprising a protein having at least 70% or more identity for the amino acid sequence represented by SEQ ID NO:1.
2. A partial peptide of the cytotoxic protein described in claim 1, characterized in that the protein has the same cytotoxic activity as that of the amino acid sequence represented by SEQ ID NO:1.
3. The cytotoxic protein of claim 1 or claim 2 wherein the protein is produced with Helicobacter pylori.
4. The cytotoxic protein of claim 1 or claim 2 wherein the protein is obtained by culturing a transformant which is transformed with a recombination vector containing DNA of SEQ ID NO:2 coding said cytotoxic protein or said partial peptide of said cytotoxic protein.
5. The cytotoxic protein of claim 4 wherein the transformant is deposited by deposition No. FERM BP-8218 at National Institute of Advanced Industrial Scienc and Technology (IPOD).
6. An antitumor agent which contains said cytotoxic protein of claim 1 or 2.
7. A monoclonal antibody specific against the cytotoxic protein which is obtained by immunization of said cytotoxic protein of claim 1 or 2 against a mammal.
8. The monoclonal antibody of claim 7 which is produced with a hybridoma clone of deposition No. FERM BP-8222.
9. The monoclonal antibody of claim 7 which is produced with a hybridoma clone of deposition No. FERM BP-8223.
10. The monoclonal antibody of claim 7 which is produced with a hybridoma clone of deposition No. FERM BP-8224.
11. A polyclonal antibody specific against the cytotoxic protein which is obtained by immunization of said cytotoxic protein of claim 1 or 2 against a mammal.
12. A method for detecting and diagnosing the cytotoxic protein of claim 1 or 2 wherein a monoclonal antibody or a polyclonal antibody is used, said monoclonal or polyclonal antibody being specific against the cytotoxic protein which is obtained by immunization of said cytotoxic protein against a mammal.
13. An agent for preventing and treating gastric cancer, gastritis, or gastric ulcer triggered by the cytotoxic protein of claim 1 or 2, wherein a monoclonal antibody or a polyclonal antibody are used, said monoclonal or polyclonal antibody being specific against the cytotoxic protein which is obtained by immunization of said cytotoxic protein against a mammal.
14. A method for screening a compound promoting or inhibiting activity of the cytotoxic protein of claim 1 or 2, wherein cell multiplication inhibition activity, cytotoxic activity or cell death is judged by comparison between negative or positive control groups with a warm-blooded animal cell.
15. A kit for screening a compound or a salt thereof promoting or inhibiting activity of the cytotoxic protein of claim 1 or 2, comprising said cytotoxic protein.
16. A compound or a salt thereof promoting or inhibiting activity of the cytotoxic protein of claim 1 or 2, wherein the compound is obtained by a screening method comprising comparing inhibition activity, cytotoxic activity or cell death by comparison between negative and positive control groups with a warm-blooded animal cell.
17. A medicine containing a compound or a salt thereof, wherein the compound has activity inhibiting cytotoxic activity of warm-blooded animal cells with the cytotoxic protein of claim 1 or 2.
18. A medicine which is an agent for preventing or treating gastritis, gastric cancer or a disease showing that it is caused with M toxin by the screening method of claim 14.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for recycling an organic ruthenium compound for chemical vapor deposition by extracting an unreacted organic ruthenium compound from a used raw material which has undergone a thin film formation process, wherein the used raw material comprises any of the organic ruthenium compounds having the following chemical formulae, Chemical Formula 1, Chemical Formula 2, Chemical Formula 3, and the method comprising the following steps (a) to (c), wherein order of the steps (a) to (c) is arbitrary and the step (a) and the step (b) may be carried out simultaneously:
(a) a modification step in which the used raw material and a hydrogenation catalyst are brought into contact with each other in a hydrogen atmosphere, thereby hydrogenating an oxidized organic ruthenium compound in the used raw material;
(b) an adsorption step in which the used raw material and an adsorbent are brought into contact with each other, thereby removing impurities in the used raw material; and
(c) a restoration step in which the used raw material is heated at a temperature that is not lower than \u2212100\xb0 C. and not higher than \u221210\xb0 C. with respect to the decomposition temperature of the organic ruthenium compound for eight hours or more, thereby adjusting a ratio of isomers of the organic ruthenium compound in the used raw material;
wherein Chemical Formula 1 is
wherein the substituent R is a linear chain or branched chain alkyl group;
Chemical Formula 2 is
wherein the substituent R is a linear chain or branched chain alkyl group; and
Chemical Formula 3 is
wherein the substituent R is a linear chain or branched chain alkyl group.
2. The method for recycling an organic ruthenium compound according to claim 1, wherein the hydrogenation catalyst in the step (a) is any of a palladium catalyst, a platinum catalyst, a ruthenium catalyst, and a Raney nickel catalyst.
3. The method for recycling an organic ruthenium compound according to claim 1 wherein the adsorbent in the step (b) is any of an activated carbon, silica gel, zeolite, and alumina.
4. The method for recycling an organic ruthenium compound according to claim 1, wherein in a reaction condition of the adsorbent in the step (b), an adsorbent having a specific surface area of 500 m2g to 1500 m2g, and a use amount of the adsorbent is 110 to 10 times as weight of the used raw material.
5. The method for recycling an organic ruthenium compound according to claim 1, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
6. The method for recycling an organic ruthenium compound according to claim 2, wherein the adsorbent in the step (b) is any of an activated carbon, silica gel, zeolite, and alumina.
7. The method for recycling an organic ruthenium compound according to claim 2, wherein in a reaction condition of the adsorbent in the step (b), an adsorbent having a specific surface area of 500 m2g to 1500 m2g, and a use amount of the adsorbent is 110 to 10 times as weight of the used raw material.
8. The method for recycling an organic ruthenium compound according to claim 3, wherein in a reaction condition of the adsorbent in the step (b), an adsorbent having a specific surface area of 500 m2g to 1500 m2g, and a use amount of the adsorbent is 110 to 10 times as weight of the used raw material.
9. The method for recycling an organic ruthenium compound according to claim 6, wherein in a reaction condition of the adsorbent in the step (b), an adsorbent having a specific surface area of 500 m2g to 1500 m2g, and a use amount of the adsorbent is 110 to 10 times as weight of the used raw material.
10. The method for recycling an organic ruthenium compound according to claim 2, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
11. The method for recycling an organic ruthenium compound according to claim 3, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
12. The method for recycling an organic ruthenium compound according to claim 4, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
13. The method for recycling an organic ruthenium compound according to claim 6, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
14. The method for recycling an organic ruthenium compound according to claim 7, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
15. The method for recycling an organic ruthenium compound according to claim 8, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
16. The method for recycling an organic ruthenium compound according to claim 9, wherein the organic ruthenium compound is extracted by distillation after processes by the steps (a) to (c).
17. The method for recycling an organic ruthenium compound according to claim 1 wherein the organic ruthenium compound in the used raw material has Chemical Formula 1.
18. The method for recycling an organic ruthenium compound according to claim 1 wherein the organic ruthenium compound in the used raw material has Chemical Formula 2.
19. The method for recycling an organic ruthenium compound according to claim 1 wherein the organic ruthenium compound in the used raw material has Chemical Formula 3.