What is claimed is:
1. A suspension of cells comprising pluripotent hematopoietic stem cells substantially free of c cells.
2. The cell suspension of claim 1 wherein the pluripotent hematopoietic stem cells comprise single cells having both lymphoid and myeloid potential.
3. The cell suspension of claim 2 wherein greater than about 10% of single cells comprise pluripotent hematopoietic stem cells having both lymphoid and myeloid potential.
4. The cell suspension of claim 2 wherein greater than about 5% of cells comprise pluripotent hematopoietic stem cells that proliferated late as measured by the Extended Long Term Culture-Initiating Cell (ELTC-IC) assay.
5. The cell suspension of claim 1 wherein the pluripotent hematopoietic stem cells comprise CD34 cells.
6. The cell suspension of claim 5 comprising greater than about 85% CD34 c cells.
7. The cell suspension of claim 6 comprising greater than about 85% CD34CD38 c cells.
8. The cell suspension of claim 6 comprising greater than about 85% CD34CD38 c IL-7R cells.
9. A method of purifying hematopoietic stem cells comprising
(a) obtaining a sample of cells that includes hematopoietic stem cells;
(b) separating the cells in the sample into CD34 and CD34 cells;
(c) collecting the CD34 cells;
(d) separating the CD34 cells into CD34 c and CD34 c cells;
(e) collecting the CD34 c cells.
10. The method of claim 9 wherein (d) and (e) are modified as follows
(d) separating CD34 cells into a first group of CD34 c IL-7R cells and a second group of CD34 c IL-7R, CD34 c IL-7R and CD34 c IL-7R cells;
(e) collecting the first group of cells.
11. A suspension of human cells from marrow or blood comprising CD34 c cells and substantially free of cells that are not CD34 c, said suspension having the ability to restore the production of lymphoid and hematopoietic cells to a human lacking said production.
12. A suspension of normal human cells derived from blood or bone marrow of an individual affected with leukemia comprising normal pluripotent hematopoietic stem cells substantially free of c leukemia cells, mature lymphoid cells and mature myeloid cells.
13. The suspension of normal human cells of claim 12, wherein the normal pluripotent hematopoietic stem cells are substantially free of CD34 c IL-7R, CD34c IL-7R and CD34 c IL-7R leukemia cells, mature lymphoid cells and mature myeloid cells.
14. A method of purifying normal pluripotent hematopoietic stem cells substantially free of malignant cells from an individual having a c malignancy comprising
(a) obtaining a sample of cells that includes hematopoietic stem cells from an individual having a c malignancy;
(b) separating the cells in the sample into CD34 and CD34 cells;
(c) collecting the CD34 cells;
(d) separating the CD34 cells into CD34 c and CD34 c cells;
(e) collecting the CD34 c cells.
15. The method of claim 14 wherein the individual has a c IL-7R malignancy, wherein (d) and (e) are modified as follows
(d) separating CD34 cells into a first group of CD34 c IL-7R cells and a second group of CD34 c IL-7R, CD34 c IL-7R and CD34 c IL-7R cells;
(e) collecting the first group of cells.
16. The method of claim 14, wherein the malignancy is leukemia.
17. The method of claim 16, wherein the leukemia is selected from the group consisting of acute non-lymphoblastic leukemia (ANLL) or acute lymphoblastic leukemia (ALL).
18. A kit for purifying hematopoietic stem cells, said kit having a package comprising
a means for selecting CD34 cells, said means selected from the group consisting of labeled anti-CD34 antibodies, for labeling cells for separation by a mechanical cell sorter that detects the presence of the label, and anti-CD34 antibodies attached to a first solid support;
a means for selecting c cells, said means selected from the group consisting of labeled anti-c antibodies, for labeling cells for separation by a mechanical cell sorter that detects the presence of the label, and anti-c antibodies attached to a second solid support.
19. The kit of claim 18 wherein the first solid support is selected from the group consisting of agarose beads, polystyrene beads, magnetic beads, hollow fiber membranes and plastic petri dish.
20. The kit of claim 18 wherein the second solid support is selected from the group consisting of agarose beads, polystyrene beads, magnetic beads, hollow fiber membranes, plastic support and plastic petri dish.
21. The kit of claim 18 wherein the anti-CD34 antibodies are labeled with a fluorescent label or a magnetic label.
22. The kit of claim 18 wherein the anti-c antibodies are labeled with a fluorescent label or a magnetic label.
23. The kit of claim 18 wherein the anti-c antibodies are monoclonal antibodies.
24. The kit of claim 23 wherein the anti-c antibodies are y1 monoclonal antibodies.
25. A kit for purifying normal pluripotent hematopoietic stem cells substantially free of malignant cells from an individual having a c malignancy comprising, said kit having a package comprising
a means for selecting CD34 cells, said means selected from the group consisting of labeled anti-CD34 antibodies, for labeling cells for separation by a mechanical cell sorter that detects the presence of the label, and anti-CD34 antibodies attached to a first solid support;
a means for selecting c cells, said means selected from the group consisting of labeled anti-c antibodies, for labeling cells for separation by a mechanical cell sorter that detects the presence of the label, and anti-c antibodies attached to a second solid support.
26. The kit of claim 25 wherein the first solid support is selected from the group consisting of agarose beads, polystyrene beads, magnetic beads, hollow fiber membranes and plastic petri dish.
27. The kit of claim 25 wherein the second solid support is selected from the group consisting of agarose beads, polystyrene beads, magnetic beads, hollow fiber membranes, plastic support and plastic petri dish.
28. The kit of claim 25 wherein the anti-CD34 antibodies are labeled with a fluorescent label or a magnetic label.
29. The kit of claim 25 wherein the anti-c antibodies are labeled with a fluorescent label or a magnetic label.
30. The kit of claim 25 wherein the anti-c antibodies are monoclonal antibodies.
31. The kit of claim 30 wherein the anti-c antibodies are c1 monoclonal antibodies.
32. A method of reconstituting the lympho-hematopoietic system of an individual in need thereof comprising administering an effective amount of the cell suspension of claim 1.
33. A method of reconstituting the lympho-hematopoietic system of an individual in need thereof comprising administering an effective amount of the cell suspension of claim 12.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An information processing device comprising:
a feature quantity obtaining unit configured to obtain a feature quantity of an object to be extracted, which is extracted from a retrieval target, and specific information that is to be specified an appearing location of the feature quantity in the retrieval target;
a feature quantity holding unit configured to, when storing the feature quantity in a feature quantity table which holds the feature quantity obtained by the feature quantity obtaining unit with identification information added thereto, reference the feature quantity table, add new identification information to the feature quantity and hold the feature quantity in the feature quantity table when a similar feature quantity in which a similarity with the feature quantity is greater than or equal to a first similarity is not held in the feature quantity table, and output identification information which is the same as the similar feature quantity as identification information of the feature quantity when the similar feature quantity is held in the feature quantity table; and
a retrieval table holding unit configured to hold the specific information obtained by the feature quantity obtaining unit as a retrieval table in association with the identification information added by or the identification information outputted by the feature quantity holding unit for the feature quantity.
2. The information processing device according to claim 1, further comprising:
a first retrieving unit configured to receive a target feature quantity of an object to be specified, reference the feature quantity table, retrieve a feature quantity in which a similarity with the target feature quantity is greater than or equal to the first similarity in the feature quantity table, and obtain identification information corresponding to the found feature quantity from the feature quantity table; and
a second retrieving unit configured to reference the retrieval table, retrieve the identification information obtained by the first retrieving unit in the retrieval table, and obtain specific information corresponding to the found identification information from the retrieval table.
3. The information processing device according to claim 2, wherein
the retrieval table holding unit further holds the feature quantity obtained by the feature quantity obtaining unit in the retrieval table in association with the identification information, in addition to the specific information; and
when the first retrieving unit receives the target feature quantity of the object to be specified,
the first retrieving unit references the feature quantity table, retrieves a feature quantity in which a similarity with the received target feature quantity is greater than or equal to a second similarity which is lower than the first similarity in the feature quantity table, and obtains identification information corresponding to the found feature quantity from the feature quantity table,
the second retrieving unit references the retrieval table, retrieves the identification information obtained by the first retrieving unit in the retrieval table, and obtains a feature quantity corresponding to the found identification information from the retrieval table, and
when the feature quantity is found in plurals by the second retrieving unit, the second retrieving unit computes a similarity with the target feature quantity for each of the plurality of found feature quantities, and obtains the specific information correlated with the feature quantity in which the computed similarity is greater than or equal to the first similarity from the retrieval table.
4. The information processing device according to claim 2, wherein
when the first retrieving unit receives the target feature quantity of the object to be specified,
the first retrieving unit references the feature quantity table, retrieves a feature quantity in which a similarity with the received target feature quantity is greater than or equal to a second similarity which is lower than the first similarity in the feature quantity table, and obtains identification information corresponding to the found feature quantity from the feature quantity table, and
the second retrieving unit references the retrieval table, retrieves the identification information obtained by the first retrieving unit in the retrieval table, and obtains specific information corresponding to the found identification information from the retrieval table; and
when the specific information is found in plurals by the second retrieving unit,
the feature quantity obtaining unit obtains, in plurals, a feature quantity of the appearing location which is specified with the plurality of specific information obtained by the second retrieving unit from the retrieval target, and associates the plurality of feature quantities with the corresponding identification information, and
the second retrieving unit computes a similarity with the target feature quantity for each of the plurality of feature quantities obtained by the feature quantity obtaining unit, obtains the feature quantity in which the computed similarity is greater than or equal to the first similarity, and obtains the corresponding specific information from the identification information corresponding to the obtained feature quantity.
5. The information processing device according to claim 1, further comprising:
a table generating unit configured to, when a capacity of the feature quantity table becomes greater than or equal to a threshold, divide the feature quantity table or create a new feature quantity table to generate a feature quantity table for saving and a feature quantity table for update; and
wherein the feature quantity holding unit uses the feature quantity table for update.
6. The information processing device according to claim 5, further comprising:
a table updating unit configured to hold a usage state of the identification information at time of referencing the feature quantity table or the retrieval table, and move data of the identification information which is not used the most from the feature quantity table for update to the feature quantity table for saving based on the usage state using an LRU (Least Recently Used) method.
7. A data processing method using an information processing device, comprising:
obtaining a feature quantity of an object to be extracted, which is extracted from a retrieval target, and specific information that is to be specified an appearing location of the feature quantity in the retrieval target;
when storing the feature quantity in a feature quantity table which holds the obtained feature quantity with identification information added thereto, referencing the feature quantity table, adding new identification information to the feature quantity and holding the feature quantity in the feature quantity table when a similar feature quantity in which a similarity with the feature quantity is greater than or equal to a threshold is not held in the feature quantity table, and outputting identification information which is the same as the similar feature quantity as identification information of the feature quantity when the similar feature quantity is held in the feature quantity table; and
holding the obtained specific information as a retrieval table in association with the identification information added or outputted for the feature quantity.
8. The data processing method using the information processing device according to claim 7, further comprising:
receiving a target feature quantity of an object to be specified, referencing the feature quantity table, retrieving a feature quantity in which a similarity with the target feature quantity is greater than or equal to the threshold in the feature quantity table, and obtaining identification information corresponding to the found feature quantity from the feature quantity table; and
referencing the retrieval table, retrieving the obtained identification information in the retrieval table, and obtainging specific information corresponding to the found identification information from the retrieval table.
9. A non-transitory computer-readable recording medium storing a program for causing a computer to execute procedures of:
obtaining a feature quantity of an object to be extracted, which is extracted from a retrieval target, and specific information that is to be specified an appearing location of the feature quantity in the retrieval target;
when storing the feature quantity in a feature quantity table which holds the obtained feature quantity with identification information added thereto, referencing the feature quantity table, adding new identification information to the feature quantity and holding the feature quantity in the feature quantity table when a similar feature quantity, in which a similarity with the feature quantity is greater than or equal to a threshold, is not held in the feature quantity table, and outputting identification information which is the same as the similar feature quantity as identification information of the feature quantity when the similar feature quantity is held in the feature quantity table; and
holding the obtained specific information as a retrieval table in association with the identification information added or outputted for the feature quantity.
10. The non-transitory computer-readable recording medium according to claim 9, wherein the program further causes the computer to execute the procedures of:
receiving a target feature quantity of an object of to be specified, referencing the feature quantity table, retrieving a feature quantity in which a similarity with the target feature quantity is greater than or equal to the threshold in the feature quantity table, and obtaining identification information corresponding to the found feature quantity from the feature quantity table; and
referencing the retrieval table, retrieving the obtained identification information in the retrieval table, and obtaining specific information corresponding to the found identification information from the retrieval table.