1. A method for opening a fuel dispenser, comprising:
opening a lower panel on the fuel dispenser that secures a lower compartment of the fuel dispenser;
disengaging a hidden latch assembly located behind the lower panel that is coupled to an upper panel of the fuel dispenser to disengage the upper panel, wherein the upper panel secures an upper compartment of the fuel dispenser and is located adjacent to the lower compartment; and
opening the upper panel after the step of disengaging.
2. The method of claim 1 wherein opening the lower panel comprises unlocking a lower panel lock that secures the lower panel to the lower compartment of the fuel dispenser.
3. The method of claim 1 wherein disengaging the hidden latch assembly further comprises disengaging a ski-boot latch from a hook coupled to the upper panel.
4. The method of claim 3 wherein disengaging the hidden latch assembly further comprises removing a pad-lock from a pad-lock acceptor on the ski boot latch that blocks operation of the ski-boot latch when the pad-lock is inserted into the pad-lock acceptor.
5. The method of claim 4 wherein disengaging the ski-boot latch from the hook further prevents the lower panel from being closed because of interference between the hook and the lower panel.
6. The method of claim 1 wherein opening the upper panel further comprises disengaging a cam locking assembly from a bracket coupled to the upper compartment of the fuel dispenser.
7. The method of claim 6 wherein the cam locking assembly further comprises a lock and disengaging the cam locking assembly from the bracket further comprises inserting a key into the lock and unlocking the lock, wherein unlocking the lock disengages a cam from the bracket and retains the key in the lock.
8. The method of claim 7 further comprising preventing the upper panel from being closed when the lock is in a locked position.
9. A method for closing a fuel dispenser, comprising:
closing an upper panel of the fuel dispenser that secures an upper compartment of the fuel dispenser;
engaging a hidden latch assembly located within a lower compartment of the fuel dispenser that is coupled to the upper panel and secures the upper panel closed when engaged; and
closing a lower panel that secures the lower compartment of the fuel dispenser and hides the hidden latch assembly.
10. A fuel dispenser for providing multi-layered security for a fuel dispenser electronic compartment, comprising:
a housing;
an upper panel adapted to secure an upper compartment in the housing;
a hidden latch assembly located within a lower compartment of the housing and adapted to couple the upper panel when engaged; and
a lower panel adapted to secure the lower compartment and to hide the hidden latch assembly such that the upper panel can be opened by opening the lower panel and disengaging the hidden latch assembly.
11. The fuel dispenser of claim 10 wherein the hidden latch assembly comprises a ski-boot latch and a hook ring, and is adapted to couple the upper panel to the fuel dispenser when the hook ring of the ski-boot latch engages a hook that is secured to the upper panel.
12. The fuel dispenser of claim 11 wherein the ski-boot latch further comprises a slot and the hidden latch assembly further comprises a pad-lock acceptor adapted to pass through the slot when the ski-boot latch is engaged from a disengaged position and to receive a pad-lock to prevent the ski-boot latch from being disengaged when the pad-lock is in place.
13. The fuel dispenser of claim 10 wherein the hidden latch assembly is further adapted to prevent the lower panel from being closed when the hidden latch assembly is in a disengaged position.
14. The fuel dispenser of claim 13 wherein the hidden latch assembly prevents the lower panel from being closed when in the disengaged position by interference of a hook that is coupled to the upper panel with the lower panel.
15. The fuel dispenser of claim 10 wherein the upper panel further comprises a weather seal adapted to exert pressure against the upper panel to prevent the upper panel from being closed when the hidden latch assembly is in a disengaged position.
16. The fuel dispenser of claim 10 wherein the upper panel further comprises a cam locking assembly that further secures the fuel dispenser electronic compartment.
17. The fuel dispenser of claim 16 wherein the cam locking assembly further comprises a lock brace coupled to the fuel dispenser electronic compartment and a lock wherein the lock brace is adapted to prevent opening and closing of the upper panel when the lock is in a locked position.
18. The fuel dispenser of claim 17 wherein the cam locking assembly is further adapted to cause the upper panel to compress a weather seal associated with the upper panel against the fuel dispenser electronic compartment when the lock is engaged with the lock brace and is locked from an unlocked position.
19. The fuel dispenser of claim 16 wherein the cam locking assembly further comprises a lock and the cam locking assembly is further adapted to prevent removal of a key from the lock when the lock is in an unlocked position.
20. The fuel dispenser of claim 16 wherein the cam locking assembly further comprises a lock and the cam locking assembly is further adapted to prevent opening and closing of the upper panel when the lock is in a locked position.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A hypoxia inducible factor (HIF) mutein polypeptide wherein one or more of a hydroxylatable amino acid residue in a wild type HIF polypeptide are mutated, said HIF mutein displaying decreased binding to VHL, increased resistance to degradation in the presence of oxygen and comprises both functional wild type HIFA polypeptide transactivation domains.
2. The HIF mutein polypeptide of claim 1, wherein said hydroxylatable amino acid residue is a proline.
3. The HIF mutein polypeptide of claim 2, wherein said proline is at position 564 in wild type HIF, numbered in accordance with wild type HIF.
4. A hypoxia inducible factor (HIF) mutein polypeptide wherein the amino acid at position 564 in wild type HIF, numbered in accordance with wild type HIF is not a proline, said HIF mutein displaying decreased binding to VHL and increased resistance to degradation in the presence of oxygen.
5. A hypoxia inducible factor (HIF) mutein polypeptide comprising the amino acid sequence at least 85% similar to wild type HIF, wherein the amino acid at position 564 in wild type HIF, numbered in accordance with wild type HIF is not a proline, said HIF mutein displaying decreased binding to VHL and increased resistance to degradation in the presence of oxygen.
6. A hypoxia inducible factor (HIF) mutein polypeptide wherein the amino acid at position 562 in wild type HIF, numbered in accordance with wild type HIF is not a leucine, said HIF mutein displaying decreased binding to VHL and increased resistance to degradation in the presence of oxygen.
7. A hypoxia inducible factor (HIF) mutein polypeptide comprising the amino acid sequence at least 85% similar to wild type HIF, wherein the amino acid at position 562 in wild type HIF, numbered in accordance with wild type HIF is not a leucine, said HIF mutein displaying decreased binding to VHL and increased resistance to degradation in the presence of oxygen.
8. The HIF mutein polypeptide of claim 4, 5, 6, or 7 with an additional substitution such that the amino acid at position 402 is not a proline.
9. The HIF mutein polypeptide of claim 4 or 5, wherein the amino acid at position 564 is an alanine.
10. The HIF mutein polypeptide of claim 6 or 7, wherein the amino acid at position 562 is an alanine.
11. A HIF mutein polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4.
12. A nucleic acid encoding a polypeptide of claim 1-10 or 11.
13. The nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO:3).
14. An expression vector comprising a nucleic acid of claim 12 or 13 operably linked to a promoter.
15. A host cell containing the expression vector of claim 14.
16. A pharmaceutical composition comprising the HIF mutein any one of claims 1-11 and a pharmaceutically acceptable carrier.
17. A pharmaceutical composition comprising nucleic acid of claim 12 or 13 and a pharmaceutically acceptable carrier.
18. An antibody that specifically binds to the HIF mutein any one of claims 1-11.
19. A method for increasing the expression of at least one gene in a cell whose expression is activated by a HIF polypeptide comprising contacting the cell with the expression vector or claim 14.
20. The method of claim 16, wherein the cell is provide in vivo, in vitro or ex vivo.
21. A method of reducing, preventing or treating hypoxia or ischemia-related tissue damage in a subject having or at risk of having such damage comprising administering to the subject a therapeutically effective amount of:
(a) the nucleic acid sequence of claim 12 or 13 or
(b) the polypeptide of claim 1-10 or 11
thereby reducing the tissue damage.
22. A method of increasing angiogenesis or vascularization in a subject, comprising administering to the subject a therapeutically effective amount of:
(a) the nucleic acid sequence of claim 12 or 13 or
(b) the polypeptide of claim 1-10 or 11
thereby increasing a angiogenesis or vascularization.