1. A method for proliferating cardiomyocytes andor cardiac progenitor cells, comprising contacting cultured cardiomyocytes andor cardiac progenitor cells with GSK3\u03b2 inhibitor, and p38 inhibitor, wherein the cardiomyocyte is a human cardiomyocyte and the cardiac progenitor cell is a human cardiac progenitor cell.
2. The method according to claim 1, wherein said cardiomyocytes andor cardiac progenitor cells are cardiomyocytes andor cardiac progenitor cells obtained by differentiation induction of pluripotent stem cells.
3. The method according to claim 1, wherein said cardiomyocytes andor cardiac progenitor cells are further contacted with i) CaMK2 inhibitor, andor ii) ERK activator.
4. The method according to claim 3, wherein said CaMK2 inhibitor is KN62 or KN93, and said ERK dephosphorylation inhibitor is SU1498.
5. The method according to claim 1, wherein expression of CyclinA2, CyclinD2, CyclinD3, Cdk2 and cdk4 is enhanced and expression of Ink4b is decreased in cardiomyocytes andor cardiac progenitor cells after contacting cardiomyocytes andor cardiac progenitor cells with GSK3 beta inhibitor, and p38 inhibitor.
6. The method according to claim 1, wherein said GSK3\u03b2 inhibitor is CHIR99021 or BIO and said p38 inhibitor is SB203580.
7. A method for screening a cardiomyocyte- andor cardiac progenitor cell-proliferating agent, comprising the steps of:
(1) contacting a test substance with cardiomyocytes andor cardiac progenitor cells obtained by differentiation induction of pluripotent stem cells;
(2) counting the number of cardiomyocytes andor cardiac progenitor cells after step (1); and
(3) selecting the test substance as a cardiomyocyte- andor cardiac progenitor cell-proliferating agent when the number of cardiomyocytes andor cardiac progenitor cells is equivalent to or larger than the number of cardiomyocytes andor cardiac progenitor cells which are contacted with a positive control agent having cardiomyocyte- andor cardiac progenitor cell-proliferating ability, wherein the cardiomyocyte is a human cardiomyocyte and the cardiac progenitor cell is a human cardiac progenitor cell,
and wherein said positive control agent is at least one compound selected from the group consisting of ERK dephosphorylation inhibitor, Raf activator, and CaMK2 inhibitor.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. Monitoring system for a driver’s braking habits comprising:
a sensor means connected to a main line of a braking system of a vehicle,
said sensor means are provided to sense and evaluate the driver’s braking habits characterized by the following levels of pressure being applied to said brakes by said river:
a normal pressure level;
an above normal pressure level; and
an excessive pressure level;
said sensor means are adapted to activate a warning signal means connected to said sensor means, wherein said warning signal means are provided to warn said driver only when the pressure level exceeds the above normal pressure level and the excessive pressure level, thus facilitating correction of bad driving habits of said driver and development of preventive driving techniques.
2. Monitoring system according to claim 1, wherein said sensor means comprise a first sensor means, said first sensor means being adapted to sense and evaluate the above normal pressure level; and a second sensor means, said second sensor means being adapted to sense and evaluate the excessive pressure level.
3. Monitoring system according to claim 2, wherein each of said first and said second sensor means are connected to a respective first and second warning signal means.
4. Monitoring system according to claim 3, wherein said first and said second warning signal means are light signals.
5. Monitoring system according to claim 3, wherein said first and said second warning signal means are buzzer signals.
6. Monitoring system according to claim 2, wherein said first and said second sensor means are pressure sensors.
7. Monitoring system according to claim 1, wherein said system further comprises a first and a second event counter, said first and said second event counters are connected to said sensor means and are adapted to monitor and count the number of times when the above normal and the excessive pressure levels were applied to said brakes.
8. Monitoring system according to claim 7, wherein each of said first and said second event counters are provided with debouncing elements, wherein said debouncing elements are provided to prevent said counters from counting more than one event at a time.
9. Monitoring system according to claim 1, wherein said system further comprises an electronic control unit, said electronic control unit being connected between said sensor means and said warning signal means, wherein said electronic control unit is provided to monitor said system and to record all information.
10. Monitoring system according to claim 9, wherein said system further comprises a communication port, said communication port is connected to said electronic control unit and provided to download all information recorded into said electronic control unit.
11. Monitoring system according to claim 10, wherein said system further comprises a satellite port, said satellite port being connected to said electronic control unit and is adapted to transmit all information recorded in said electronic control unit to a remote location.