What is claimed is:
1. A method of isolating genomic deoxyribonucleic acid from whole blood, comprising:
providing a biological fluid comprising a red blood cell and a white blood cell, wherein the white blood cell has a genomic deoxyribonucleic acid and a protein;
lysing the red blood cell by adding a first aqueous solution to the biological fluid, thereby forming a lysis mixture;
removing the white blood cell from the lysis mixture, and washing the white blood cell with a second aqueous solution;
lysing the white blood cell by adding a third aqueous solution to the white blood cell, thereby forming an extraction mixture;
precipitating the protein from the extraction mixture by adding a fourth aqueous solution; and
removing the precipitated protein from the extraction mixture, thereby forming a nucleic acid solution comprising the genomic deoxyribonucleic acid, wherein the nucleic acid solution is substantially free of a polymerase inhibitor.
2. The method of claim 1 wherein the first and second aqueous solutions are the same.
3. The method of claim 1 wherein the first, second, third, and fourth aqueous solution are free of a chaotropic salt and an organic solvent.
4. The method of claim 3 wherein the biological fluid comprises whole blood.
5. The method of claim 3 wherein the first aqueous solution comprises a first inorganic salt and a second inorganic salt.
6. The method of claim 5 wherein the first aqueous solution substantially consists of about 150 mM ammonium chloride, about 10 mM sodium bicarbonate, and about 0.1 mM sodium ethylenediaminetetraacetic acid.
7. The method of claim 3 wherein the step of removing the white blood cell and the step of removing the precipitated protein comprises centrifugation.
8. The method of claim 3 wherein the third aqueous solution comprises a detergent.
9. The method of claim 8 wherein the third aqueous solution substantially consists of about 0.1 wt % sodium lauryl sulfate.
10. The method of claim 3 wherein the fourth aqueous solution comprises an organic salt.
11. The method of claim 10 wherein the fourth aqueous solution substantially consists of 5M ammonium acetate.
12. The method of claim 3 wherein the first and second aqueous solution substantially consist of about 150 mM ammonium chloride, about 10 mM sodium bicarbonate, and about 0.1 mM sodium ethylenediaminetetraacetic acid, the third aqueous solution substantially consists of about 0.1 wt % sodium lauryl sulfate, and the fourth aqueous solution substantially consists of 5M ammonium acetate.
13. The method of claim 3 further comprising precipitating the genomic deoxyribonucleic acid by adding isopropanol to the nucleic acid solution.
14. The method of claim 3 wherein at least one of the steps of providing a biological fluid, lysing the red blood cell, removing the white blood cell, lysing the white blood cell, precipitating the protein, and removing the precipitated protein is automated.
15. A kit for purifying genomic deoxyribonucleic acid from whole blood, comprising:
a first aqueous solution comprising a first inorganic salt and a second inorganic salt, wherein the first aqueous solution lyses a red blood cell;
a second aqueous solution comprising a detergent, wherein the second aqueous solution lyses a white blood cell;
a third aqueous solution comprising an organic salt, wherein the third aqueous solution precipitates a protein; and
wherein a genomic nucleic acid isolated from whole blood following a procedure that employs the first, second and third aqueous solution is substantially free of a polymerase inhibitor.
16. The kit of claim 14 wherein the first aqueous solution substantially consists of about 150 mM ammonium chloride, about 10 mM sodium bicarbonate, and about 0.1 mM sodium ethylenediaminetetraacetic acid.
17. The kit of claim 14 wherein the second aqueous solution substantially consists of about 0.1 wt % sodium lauryl sulfate.
18. The kit of claim 14 wherein the third aqueous solution substantially consists of 5M ammonium acetate.
19. The kit of claim 14 wherein the first aqueous solution substantially consists of about 150 mM ammonium chloride, about 10 mM sodium bicarbonate, and about 0.1 mM sodium ethylenediaminetetraacetic acid, the second aqueous solution substantially consists of about 0.1 wt % sodium lauryl sulfate, and the third aqueous solution substantially consists of 5M ammonium acetate.
20. The kit of claim 14 further comprising isopropanol.
21. The kit of claim 14 further comprising a printed protocol comprising instructions according to claim 1.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An electronic device comprising:
a connector configured to connect to an external device;
a controller configured to determine whether the external device is a Surface Acoustic Wave (SAW) device, to output a request signal to the SAW device through the connector when the external device is the SAW device, to receive a response signal corresponding to the request signal from the SAW device through the connector, and to process the response signal; and
an output interface unit configured to output data according to a result of processing the response signal.
2. The electronic device of claim 1, wherein the controller comprises:
a SAW device controller configured to transmit the request signal to is the SAW device, and to receive the response signal from the SAW device;
a switch configured to switch a path of a signal;
a processor configured to control the switch so that a signal inputoutput unit is connected to the connector when it is determined that the external device is the SAW device based on an IDentification (ID) value of the external device, and to process the response signal.
3. The electronic device of claim 2, wherein the response signal includes an ID code for recognizing a type of the SAW device, and detection information on a physical change or a chemical change.
4. The electronic device of claim 3, wherein the controller is further configured to control the SAW device controller to selectively read the detection information from sensors when a deployment structure of the sensors of the SAW device is parallel based on the determination of the identification code.
5. The electronic device of claim 1, further comprising a power managing unit configured to supply power of a battery to the SAW device when a power terminal of the SAW device is connected to a power terminal of the connector.
6. The electronic device of claim 2, wherein the processor is further configured to recognize that the SAW device has a wireless communication function, and to transmit and receive a signal to and from a wireless communication sensor through the SAW device.
7. The electronic device of claim 6, wherein the SAW device is included in the electronic device.
8. The electronic device of claim 2, wherein:
the connector comprises an ear jack, and
the processor is further configured to control the SAW device to transmit and receive a signal to determine whether the external device is the SAW device, when the external device is connected to the ear jack.
9. The electronic device of claim 2, wherein the SAW device controller is further configured to transmit the request signal to the SAW device through one of a plurality of data terminals of the connector, and to receive the response signal through another one of the plurality of data terminals.
10. The electronic device of claim 1, wherein the request signal and the response signal are one of a single-ended signal and a differential signal.
11. A method of operating an interface with an external device in an electronic device, the method comprising the steps of:
recognizing a connection of the external device to the electronic device;
determining whether the external device is a Surface Acoustic Wave (SAW) device;
transmitting a request signal to the SAW device when the external device is the SAW device;
receiving a response signal corresponding to the request signal from the SAW device; and
processing the response signal; and
outputting data according to the processed response signal.
12. The method of claim 11, wherein transmitting the request signal comprises controlling a switch so that a SAW device controller of the electronic device is connected to the SAW device.
13. The method of claim 11, wherein receiving the response signal comprises recognizing a type of the SAW device based on an IDentification (ID) code included in the response signal.
14. The method of claim 13, wherein:
recognizing the type of the SAW device comprises recognizing that the SAW device includes sensors having a parallel arrangement structure,
the request signal has a frequency band to which at least one of the sensors responds, and
the response signal is received from at least one of the sensors.
15. The method of claim 13, wherein recognizing the type of the SAW device comprises recognizing that the SAW device has a wireless communication function, and
further comprising transmitting and receiving a signal to and from a wireless communication sensor through the SAW device having the wireless communication function.
16. The method of claim 15, wherein the SAW device is in the electronic device.
17. The method of claim 11, further comprising:
transmitting a first signal to the external device through an ear jack when the external device is connected to the ear jack; and
recognizing the external device as the SAW device when the external device transmits a second signal in response to the first signal.
18. The method of claim 11, wherein:
transmitting the request signal comprises transmitting the request signal to the SAW device through one of a plurality of data terminals, and
receiving the response signal comprises receiving the response signal from the SAW device through another one of the plurality of data terminals.
19. The method of claim 11, wherein the request signal and the response signal are one of a single-ended signal and a differential signal.