1-20. (canceled)
21. A solid-state image pickup device, comprising a pixel array region, on a semiconductor substrate, where a plurality of pixels are two-dimensionally disposed in a row direction and a column direction with a first pitch, wherein
each of the pixels includes:
a photoelectric conversion region; and
a microlens for converging light into the photoelectric conversion region,
the microlenses are disposed in a disposition region such that a second pitch of the microlenses in a central region of the disposition region is smaller than the first pitch, and
a third pitch of the microlenses in a peripheral region of the disposition region is the same as the first pitch.
22. The solid-state image pickup device according to claim 21, wherein and
an outer shape of the pixel array region has a rectangular shape,
an outer shape of the disposition region has at least one curve which connects two adjacent vertexes of the disposition region and is concave with respect to a line passing through the two adjacent vertexes.
23. The solid-state image pickup device according to claim 21, wherein
a shape of a border of the central region and the peripheral region is substantially circle whose center is substantially same as a center of the pixel array region.
24. The solid-state image pickup device according to claim 21, wherein
the pixels are disposed such that a pitch between the pixels in a peripheral portion of the pixel array region is greater than a pitch between the pixels in a central portion of the pixel array region.
25. The solid-state image pickup device according to claim 21, wherein
the solid-state image pickup device is an amplifying-solid-state image pickup device.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A reagent for measuring a basophil andor a nucleated red blood cell, comprising:
(a) an alcohol having 4 to 8 total carbon atoms, and
(b) one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I):
(wherein R1 and R2 are independently an alkyl group;
R3, R4, R5 and R8 are independently a hydrogen atom or an alkyl group; and
X\u2212 is an anion), and
a compound of the general formula (II):
(wherein R7 and R8 are independently an alkyl group, wherein the alkyl group may have an acidic group which may form a salt;
R9, R10, R11 and R12 are independently a hydrogen atom or an acidic group which may form a salt; and
at least one of R7 to R12 has an acidic group which has released a proton).
2. The reagent according to claim 1, wherein the alcohol is selected from the group consisting of butanol, pentanol, heptanol, isobutanol, isoamyl alcohol, 2-phenylethanol and 2-phenoxyethanol.
3. The reagent according to claim 2, wherein an acidic group of the general formula (II) is a carboxyl group or a sulfonic group.
4. The reagent according to claim 1, wherein a counter ion of an acidic group forming a salt of the general formula (II) is an alkali metal ion or an alkylammonium ion.
5. The reagent according to claim 1, further comprising a surfactant for hemolyzing a red blood cell, and giving damage to a cell membrane of a leukocyte to such an extent that a fluorescent dye is capable of permeating therethrough.
6. The reagent according to claim 5, wherein the surfactant is a cationic surfactant.
7. The reagent according to claim 6, wherein the cationic surfactant is the type of a quaternary ammonium salt or a pyridinium salt.
8. The reagent according to claim 1, having a pH of 2.0 to 4.5.
9. The reagent according to claim 1, further comprising an aromatic organic acid.
10. The reagent according to claim 9, wherein the aromatic organic acid is selected from the group consisting of salicylic acid, salicylate, phthalic acid and phthalate.
11. A reagent kit for measuring a basophil andor a nucleated red blood cell, comprising:
a first solution containing
an alcohol having 4 to 8 total carbon atoms and
a surfactant for hemolyzing a red blood cell and giving damage to a cell membrane of a leukocyte to such an extent that a fluorescent dye is capable of permeating therethrough, and a second solution containing one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I) according to claim 1 and a compound of the general formula (II) according to claim 1.
12. The reagent kit according to claim 11, wherein the first solution contains an aromatic organic acid.
13. The reagent kit according to claim 11, wherein the first solution has a pH of 2.0 to 4.5.
14. The reagent kit according to claim 11, wherein the second solution contains an organic solvent.
15. A method for analyzing sample, comprising the steps of:
staining a blood cell in the sample by using
an alcohol having 4 to 8 total carbon atoms and
one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I) according to claim 1 and a compound of the general formula (II) according to claim 1;
irradiating the stained blood cell with light;
obtaining scattered light information and fluorescent information from the stained blood cell; and
counting a basophil andor a nucleated red blood cell in the sample based on the scattered light information and the fluorescent information.
16. The method according to claim 15, wherein the sample is mixed with the alcohol and the fluorescent dye in the staining step.
17. The method according to claim 15, in the staining step, further using a surfactant for hemolyzing a red blood cell and giving damage to a cell membrane of a leukocyte to such an extent that a fluorescent dye is capable of permeating therethrough.
18. The method according to claim 15, wherein the scattered light information is forward scattered light information.
19. The method according to claim 17, wherein the surfactant is a cationic surfactant.
20. The method according to claim 15, in the staining step, further using an aromatic organic acid.