1. (canceled)
2. A method for the treatment of cancer which spreads lymphatically or a disease caused by a lymphotrophic pathogen or for the regeneration of the immune system of an immunodeficient subject following transplantation of hematopoietic stem cells (HSC) comprising administering a pharmaceutically effective amount to a subject in need thereof of an iDC engineered to express
a) at least one cytokine which induces the self-differentiation of human dendritic cell (DC) progenitor cells into DCs; and
b) at least one antigen
wherein the iDC optionally comprises at least one vector, wherein said vector mediates the expression of said at least one cytokine and the at least on antigen.
3. The method according to claim 1, wherein the vector is a lentiviral vector.
4. The method to claim 1, wherein the lentiviral vector is integrase defective.
5. The method according to claim 1, wherein the iDC expressing at least one antigen expresses at least one cytokine which induces the self-differentiation of human dendritic cell (DC) progenitor cells into DCs.
6. The method according to claim 1, wherein the cytokine or combination of cytokines isare selected from the group consisting of GM-CSF, IL-4, IFN-\u03b1, IL-15, TGF-B, TNF-\u03b1, FLT3L, IL-3, CD40L, FLT3L and IL-3; FLT3L and CD40L; FLT3L and IFN \u03b1; GM-CSF and IFN-\u03b1 and IL-15; GM-CSF and IFN-\u03b1 and TNF-\u03b1; and GM-CSF and IFN-\u03b1 and TGF-\u03b2.
7. (canceled)
8. The method according to claim 1, wherein one antigen expressed by the iDC is an antigen which can induce a cytotoxic or humoral immune response selected from the group consisting of xeno-reactivity, allo-reactivity, neo-reactivity or auto-immunity
9. The method according to claim 1, wherein the immunodeficiency of the subject is an immunodeficiency selected from the group consisting of immunodeficiency caused by ionizing radiation, immunodeficiency caused by the administration of at least one cytotoxic pharmaceutical, primary immunodeficiency and immunodeficiency caused by a pathogen.
10-15. (canceled)
16. An iDC comprising at least one integrase-defective lentiviral vector, wherein said vector mediates expression of
a) at least one cytokine which induces the self-differentiation of human dendritic cell (DC) progenitor cells into DCs; and
b) at least one antigen.
17-22. (canceled)
23. A mouse with a regenerated immune system produced by a method according to claim 8.
24. Use of the mouse according to claim 23, for the study of the human immune system or for the testing of drugs, implants or devices for their use in humans.
25. A method for regenerating an immune system in an immunodeficient subject comprising the steps of:
a) transplanting hematopoietic stem cells to the subject; and
b) administering to the subject an induced dendritic cell (iDC) engineered to express at least one antigen and at least one cytokine which induces the self-differentiation of human dendritic cell (DC) progenitor cells into DCs.
26. The method according to claim 25, wherein the subject is a mouse and the HSC are derived from a human.
27. The method according to claim 26, wherein the mouse is characterized by the presence of endogenous T-cells and endogenous progenitors of dendritic cells.
28. The method according to claim 26, wherein the mouse strain has a primary immune deficiency that leads to dysfunction or absence of adaptive immune system (including T and B cells).
29. The method according to claim 26, wherein the mouse is selected from the group of strains consisting of NOD-Rag1nullIL2Rynull-NRG, NODLtSz-SCIDIL2Rynull-NSG and NODSCIDIL2Rynull-NOG.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A gray scale correction method for a display device which includes a color display pixel including a plurality of pixels, the method comprising the steps of:
setting a target value including a target luminance and a target chromaticity of the color display pixel;
measuring tristimulus values of each of a plurality of reference colors and each of a plurality of comparative colors in accordance with a prescribed gamma characteristic, wherein each of the plurality of reference colors represents a color of the color display pixel in the case where each one of the plurality of pixels has a reference gray scale and the other pixels each have a fixed pixel; and each of the plurality of comparative colors represents a color of the color display pixel in the case where each one of the plurality of pixels has an adjacent gray scale continuous to the reference gray scale and the other pixels each have the fixed pixel;
finding a reference value and a comparative value from the measured tristimulus values, wherein:
the reference value includes a reference luminance representing a sum of luminances of the plurality of reference colors and a reference chromaticity obtained from a sum of the tristimulus values of the plurality of reference colors; and
the comparative value includes a plurality of comparative luminances and a plurality of comparative chromaticities, wherein each of the plurality of comparative luminances represents a sum of luminances of the reference colors other than each one of the plurality of reference colors and one of the plurality of comparative colors, wherein a pixel of the one comparative color that corresponds to a pixel of the each one reference color having the reference gray scale has the adjacent gray scale; and the plurality of comparative chromaticities are each obtained from a sum of the tristimulus values of the reference colors other than the each one reference color and the one comparative color; and
correcting the reference gray scale based on the target value, the reference value and the comparative value.
2. The gray scale correction method for a display device of claim 1, wherein the step of correcting the reference gray scale includes the step of using a steepest descent method.
3. The gray scale correction method for a display device of claim 2, wherein the step of using the steepest descent method includes the step of using a function obtained by dividing the reference value by the target value and subtracting 1 from the resultant quotient.
4. The gray scale correction method for a display device of claim 1, wherein after the step of correcting the reference gray scale, the step of measuring the tristimulus values in accordance with the corrected gamma characteristic of the reference gray scale as the prescribed gamma characteristic, the step of finding the reference value and the comparative value, and the step of correcting the reference gray scale are further performed.
5. The gray scale correction method for a display device of claim 1, wherein in the step of measuring the tristimulus values, the gray scales of the pixels other than each one of the plurality of pixels are fixed to the fixed gray scale, and the gray scale of the each one pixel is varied from a minimum gray scale to a maximum gray scale.
6. The gray scale correction method for a display device of claim 1, wherein the plurality of pixels include three pixels.
7. The gray scale correction method for a display device of claim 6, wherein the three pixels include a red pixel, a green pixel and a blue pixel.
8. The gray scale correction method for a display device of claim 1, wherein:
the plurality of pixels include four pixels;
the target value further includes a target area size of a color reproduction range; and
in the step of finding the reference value and the comparative value,
the reference value further includes a reference area size of the color reproduction range defined by the plurality of reference colors; and
the comparative value further includes a plurality of comparative area sizes of the color reproduction range defined by the reference colors other than the each one reference color and the one comparative color.
9. The gray scale correction method for a display device of claim 8, wherein the four pixels include the red pixel, the green pixel, the blue pixel and a yellow pixel.
10. The gray scale correction method for a display device of claim 1, wherein:
the plurality of pixels include a first pixel, a second pixel, a third pixel, a fourth pixel and a fifth pixel;
the target value further includes a first target area size and a second target area size of the color reproduction range; and
in the step of finding the reference value and the comparative value,
the reference value further includes a first reference area size of a first part and a second area size of a second part of the color reproduction range defined by the plurality of reference colors, the first part being defined by four reference colors represented in the case where the first, second, third and fourth pixels respectively have the reference gray scale, and the second part being defined by three reference colors represented in the case where the first, fourth and fifth pixels respectively have the reference gray scale; and
the comparative value further includes a first comparative area size of the first part and a second comparative area size of a second part of the color reproduction range which is defined by the four reference colors, among the plurality of reference colors, represented in the case where any four pixels among the first through fifth pixels respectively have reference gray scales, and by one comparative color, among the plurality of comparative colors, represented in the case where the remaining one pixel has the adjacent gray scale, wherein the first part is defined by four colors represented in the case where the first, second, third and fourth pixels have the reference gray scale or the adjacent gray scale, and the second part is defined by three colors represented in the case where the first, fourth and fifth pixels have the reference gray scale or the adjacent gray scale.
11. The gray scale correction method for a display device of claim 10, wherein first pixel, the second pixel, the third pixel, the fourth pixel and the fifth pixel include the red pixel, the green pixel, the blue pixel, a yellow pixel and a cyan pixel.
12. A method for producing a display device, comprising the steps of:
preparing a display device having a prescribed gamma characteristic; and
correcting gray scales of the display device in accordance with the gray scale correction method for a display device of claim 1.
13. A method for producing a display device, comprising the steps of:
preparing a display device including a video signal input section, a signal processing section, and a display section; and
setting the signal processing section such that a gamma characteristic of the gray scale corrected in accordance with the gray scale correction method for a display device of claim 1 is exhibited.