1-5. (canceled)
6: An immunogenic composition for conferring protection in a host against disease caused by Haemophilus influenzae, which comprises:
an analog of Haemophilus influenzae Hin47 protein having a decreased protease activity which is less than about 10% of that of natural Hin47 protein, and
a high molecular weight (HMW) protein of a strain of non-typeable Haemophilus influenzae.
7: The composition of claim 6 wherein said HMW protein is present in said composition in an amount which enhances the immune response in a host to the Hin47 protein.
8: The composition of claim 7 wherein said HMW protein is present in the said amount while the individual immunogenicities of the proteins in the composition is are not impaired.
9: The composition of claim 6 wherein said analog of Hin47 protein is one in which at least one amino acid of the natural Hin47 protein contributing to protease activity has been deleted or replaced by a different amino acid and which has substantially the saem immunogenic properties as natural Hin47 protein.
10: The composition of claim 9 wherein said at least one amino acid is selected from the group consisting of amino acids 91, 121 and 195 to 201 of natural Hin47 protein.
11: The compostion of claim 10 wherein Serine-197 is replaced by alanine.
12: The compostion of claim 10 wherein Histidine-91 is replaced by alanine, lysine or arginine.
13: The compostion of claim 12 wherein Histidine-91 is replaced by alanine.
14: The composition of claim 10 wherein Asp-121 is replaced by alanine.
15: The composition of claim 8 wherein said HMW protein is an HMW1 or HMW2 protein of a non-typeable strain of Haemophilus influenzae.
16: The composition of claim 15 wherein the HMW 1 and HMW2 proteins are produced recombinantly.
17: The composition of claim 15 where said HMW1 and HMW2 proteins are derived from the respective strain of non-typeable Haemophilus influenzae and possess respective molecular weights as set forth in the following Table:
Non-typeable H. influenzae Strain
Molecular Weight (kDa)
12
JoyC
K21
LCDC2
PMH1
15
Mature Protein:
HMW1
125
125.9
104.4
114.0
102.4
103.5
HMW2
120
100.9
111.7
103.9
121.9
18: The composition of claim 6 further comprising an adjuvant.
19: The composition of claim 18 wherein said adjuvant is aluminum hydroxide or aluminum phosphate.
20: The composition of claim 6 comprising about 25 to about 100 \u03bcg of the Hin47 protein analog, and about 25 to about 100 \u03bcg of the HMW protein.
21: The composition of claim 6 further comprising at least one additional antigenic component for conferring protection against infection caused by another pathogen.
22: The composition of claim 21 wherein said at least one additional antigenic component is selected from the group consisting of diphtheria toxoid, tetanus toxoid, pertussis antigens, non-virulent poliovirus and a conjugate of a tetanus or diphtheria toxoid and a capsular polysaccharide of Haemophilus influenzae.
23: The composition of claim 22 wherein said pertussis antigens are selected from the group consisting of pertussis toxoid, filamentous hemagglutinin, pertactin and agglutinogens.
24: A method of immunzing a host against disease caused by infection with Haemophius influenzae, including otitis media, which comprises administering to the host an immunoeffective amount of a composition as claimed in claim 6.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A stacked and folded electrochemical device comprising multi-stacked unit cells of full cells or bicells and a separation film interposed therebetween and wrapping them, wherein the separators of the unit cells are formed of a porous material having a low-thermal shrinkage rate and the separation film is formed of a porous material having a relatively high-thermal shrinkage rate.
2. The electrochemical device according to claim 1, wherein the device has a structure including multi-stacked unit cells of full cells or bicells and a porous separation film interposed therebetween, wherein the porous separation film has a unit length which is determined to wrap the unit cells and folds inward every unit length to wrap each unit cell continuously starting from the central unit cell to the outermost unit cell.
3. The electrochemical device according to claim 1, wherein the device has a structure including multi-stacked unit cells of full cells or bicells and a porous separation film interposed therebetween, wherein the porous separation film has a unit length which is determined to wrap the unit cells and folds outward every unit length to fold each unit cell in a Z-shape continuously starting from the unit cell of a first position to the unit cell of the last position while the remaining separation film wraps outer portions of the stacked unit cells.
4. The electrochemical device according to claim 1, wherein the separator exhibits no thermal shrinkage until at least 150\xb0 C.
5. The electrochemical device according to claim 1, wherein the separator is formed of a ceramic material or an engineering plastic having a melting point of more than 200\xb0 C.
6. The electrochemical device according to claim 5, wherein the ceramic material is selected from the group consisting of Pb(Zr,Ti)O3, Pb1\u2212xLaxZr1\u2212yTiyO3 wherein x and y are independently in the range of 0 to 1, PB(Mg3Nb23)O3\u2014PbTiO3, BaTiO3, HfO2, SrTiO3, TiO2, SiO2, Al2O3, ZrO2, SnO2, CeO2, MgO, CaO, Y2O3 and any combination thereof.
7. The device according to claim 5, wherein the engineering plastic is selected from the group consisting of polyester, polysulfone, polyimide, polyamide, polyacetal and any combination thereof.
8. The electrochemical device according to claim 1, wherein the separation film exerts shutdown functions by thermal behavior thereof at a temperature of 100 to 150\xb0 C.
9. The electrochemical device according to claim 8, wherein the separation film exerts shutdown functions by thermal behavior thereof at a temperature of 120 to 140\xb0 C.
10. The electrochemical device according to claim 1, wherein the separation film is formed of a polyolefin resin, or a copolymer or blend thereof.
11. The electrochemical device according to claim 1, wherein the separation film is formed of a porous material uniaxially oriented in the folding direction, such that thermal shrinkage takes place only in the folding direction.
12. The electrochemical device according to claim 1, wherein the device is a lithium secondary battery.
13. An electrochemical device pack comprising one or more electrochemical devices of claim 1.
14. The electrochemical device pack according to claim 13, wherein the electrochemical devices are connected in a serial or parallel manner.