1. Gene drug for Hemophilia B contains gene vector-FIX recombinant constructed by DNA sequence as leading sequence of therapy gene without important psychological function related gene on the short arms of human group D, G chromosomes or to which DNA sequence is homologous.
2. Gene drug for Hemophilia B according to the claim 1 wherein the said vector has the DNA sequences as SEQ NO. 1, and the insert site of FIX therapy gene is 5910.
3. Gene drug for Hemophilia B according to the claim 1 or 2 which contains in vitro human cells which contain the vector-FIX recombinant as the Right claim I and II claim.
4. Preparation method of gene drug for therapy hemophilia B contains following procedures:
(1) construct the gene vector using the DNA sequences without important physiological function-related gene or to which DNA sequence is homologous on the short arms of human group D,G chromosomes as the leading sequence for gene therapy.
(2) Transduce the FIX therapy gene into the above gene vector to obtain the vector-FIX recombinant.
5. Preparation method of gene drug for therapy hemophilia B according to claim 4 wherein:
(1) The leading sequence of gene therapy select from DNA sequence without importance physiological function- related gene DNA Sequence, length of 3.8 Kb ,insert positive selection gene Neo into site 1500 of leading sequence of therapy gene, yielding two arms of 1.5 kb and 2.3 kb using TK as negative selection gene on the short arms of human D,G groups chromosomes construct gene vector.
(2) The inserting site of the FIX gene therapy is 5910.
6. Preparation method of gene drug for therapy hemophilia B according to claim 4 or 5 wherein transferring the vector-FIX recombinant into in vitro human cell.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A cover for a liquid injection container comprising an inner plug and a cap,
said inner plug comprising an engaging portion to be fitted to a neck of the container, a threaded cylinder and a lip cylinder connected to said threaded cylinder,
said engaging portion comprising an inner cylinder, an outer cylinder, and a shoulder wall,
said threaded cylinder being arranged on the inner cylinder,
said cap having a side cylinder to be engaged with said threaded cylinder of said inner plug, and a cover cylinder covering said shoulder wall, and said outer cylinder,
said cover cylinder having a diameter larger than a diameter of said side cylinder,
said outer cylinder being provided with a cut portion,
said shoulder wall being provided with a cut portion connecting to said cut portion of the outer cylinder,
said shoulder wall being provided on a lower surface thereof with a tear off groove along the outer cylinder, and
said groove being connected to said cut portion of the shoulder wall.
2. A cover for a liquid injection container comprising an inner plug and a cap,
said inner plug comprising an engaging portion to be fitted to a neck of the container, a threaded cylinder, and a lip cylinder connected to said threaded cylinder,
said engaging portion comprising an inner cylinder, an outer cylinder, and a shoulder wall,
said threaded cylinder being arranged on the inner cylinder,
said cap having a side cylinder to be engaged with said threaded cylinder of said inner plug, and a cover cylinder covering said shoulder wall and said outer cylinder,
said cover cylinder having a diameter larger than a diameter of said side cylinder,
said outer cylinder having a connecting portion, and being provided with a cut portion,
said shoulder wall being provided with a cut portion connecting to said cut portion of the outer cylinder,
said shoulder wall being provided on a lower surface thereof with a tear off groove along the outer cylinder,
said groove being connected to said cut portion of the shoulder wall, and
an lower end of said outer cylinder extending beyond a lower end of the cylindrical wall of the cap.
3. A cover for a liquid injection container comprising an inner plug and a cap,
said inner plug comprising an engaging portion to be fitted to a neck of the container, a threaded cylinder, and a lip cylinder connected to said threaded cylinder,
said engaging portion comprising an inner cylinder, an outer cylinder, and a shoulder wall,
said threaded cylinder being arranged on the inner cylinder,
said cap having a side cylinder to be engaged with said threaded cylinder of said inner plug, and a cover cylinder covering said shoulder wall, and said outer cylinder,
said cover cylinder having a diameter larger than a diameter of said side cylinder,
said outer cylinder having a connecting portion, and being provided with a cut portion,
said shoulder wall being provided with a cut portion connecting to said cut portion of the outer cylinder,
said shoulder wall being provided on a lower surface thereof with a tear off groove along the outer cylinder,
said groove being connected to said cut portion of the shoulder wall, and
the outer cylinder being provided on an outer surface thereof at a lower end thereof with a flange.
4. A cover member of a liquid injection container according to claims 2 or 3, wherein a color of the inner plug is different from a color of the cap.
5. A cover member of a liquid injection container according to claims 1, 2 or 3, wherein the cap is molded from a transparent or a semitransparent synthetic resin.
6. A jig for holding an inner plug unrotatably, said inner plug comprising a lip cylinder, a threaded cylinder, and an engaging portion, said engaging portion comprising an outer cylinder, and an inner cylinder,
said inner plug being provided with a lot of longitudinal ribs between the lip cylinder and the threaded cylinder,
said jig comprising a column and a cylinder, said column having a diameter substantially same as a diameter of an inner surface of said inner cylinder, said cylinder extending from an outer periphery of the column and having a thickness substantially same as a space between the lip cylinder and the threaded cylinder,
said cylinder being provided with slits corresponding to said longitudinal ribs.
7. A jig for holding an inner plug unrotatably, said inner plug comprising a lip cylinder, a threaded cylinder, and an engaging portion, said engaging portion comprising an outer cylinder and an inner cylinder,
said lip cylinder having a partition wall, said partition wall being provided on a lower surface thereof with an inner wall aligned and below the lip cylinder, said inner wall being formed with vertical ribs on an inner surface thereof,
said jig comprising a holding base and a support step for receiving a lower end of the inner wall of the inner plug,
said holding base being provided with vertical grooves which engage with the vertical ribs, said holding base having an outer surface which engages with an inner surface of the inner wall of the inner plug.