1. A method for increasing the production of a bacteriocin comprising:
(a) adding a bacteriocin producing bacteria with an inducer bacteria to a culture system,
(b) adding a peptide having a carboxy terminal amino acid sequence of SEQ ID NO: 1, and
(c) culturing said bacteria with said peptide for a time sufficient to achieve increased production of said bacteriocin.
2. A method for stimulating the production of the bacteriocin having SEQ ID NO: 2, comprising
(a) adding the bacteriocin producing bacterial strain of L. salivarius NRRL B-30514 with inducer bacterial strain L. crispatus NRRL B-30884 to a culture system,
(b) adding a peptide having SEQ ID NO: 3, and
(c) culturing said bacteria with said peptide for a time sufficient to achieve increased production of said bacteriocin.
3. A method for stimulating the production of the bacteriocin having SEQ ID NO: 4 comprising:
(a) adding the bacteriocin producing bacterial strain of E. facecium NRRL B-30746 with inducer bacterial strain L. crispatus NRRL B-30884 to a culture system,
(b) adding a peptide having SEQ ID NO: 5, and
(c) culturing said bacteria with said peptide for a time sufficient to achieve increased production of said bacteriocin.
4. A method for stimulating the production of the bacteriocin having SEQ ID NO: 6 comprising
(a) adding the bacteriocin producing bacterial strain of S. cricetus NRRL B-30745 with inducer bacterial strain L. acidophilus NRRL B-30510 to a culture system,
(b) adding a peptide having SEQ ID NO: 7, and
(c) culturing said bacteria with said peptide for a time sufficient to achieve increased production of said bacteriocin.
5. A method for stimulating the production of the bacteriocin having SEQ ID NO: 2 comprising
(a) adding the bacteriocin producing bacterial strain of L. salivarius NRRL B-30514 with inducer bacterial strain L. crispatus NRRL B-30884 to a culture system,
(b) adding a peptide having a carboxy terminal amino acid sequence of SEQ ID NO: 1, and
(c) culturing said bacteria with said peptide for a time sufficient to achieve increased production of said bacteriocin.
6. A method for stimulating the production of the bacteriocin having SEQ ID NO: 4 comprising
(a) adding the bacteriocin producing bacterial strain of E. faecium NRRL B-30746 with inducer bacterial strain L. acidophilus NRRL B-30510 to a culture system,
(b) adding a peptide having a carboxy terminal amino acid sequence of SEQ ID NO: 1, and
(c) culturing said bacteria with said peptide for a time sufficient to achieve increased production of said bacteriocin.
7. A method for stimulating the production of the bacteriocin having SEQ ID NO: 6 comprising
(a) adding the bacteriocin producing bacterial strain of S. cricetus NRRL B-30745 with inducer bacterial strain L. acidophilus NRRL B-30510 to a culture system,
(b) adding a peptide having a carboxy terminal amino acid sequence of SEQ ID NO: 1, and
(c) culturing said bacteria with said peptide for a time sufficient to achieve increased production of said bacteriocin.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of manufacturing an organic solar photovoltaic array; comprising the steps:
forming a plurality of organic solar photovoltaic cells, further comprising:
patterning ITO onto a substrate;
applying a Self Assembled Monolayer layer onto the etched ITO glass, wherein the Self Assembled Monolayer layer comprises N-propyl trimethoxysilane or aminopropyl triethoxysilane;
annealing the Self Assembled Molecule layer inside a glovebox;
spraying an active layer of P3HT and PCBM on the Self Assembled Molecule layer;
drying the solar photovoltaic cell in an antechamber under vacuum for at least 12 hours;
spraying a layer comprising poly (3,4) ethylenedioxythiophene:poly-styrenesulfonate mixed with 5 vol. % of dimethylsulfoxide on the active layer;
placing the solar photovoltaic cell into high vacuum for 1 h;
annealing the solar photovoltaic cell;
encapsulating the solar photovoltaic cell with a UV-cured epoxy; and
integrating the plurality of organic solar photovoltaic cells into an array as depicted in FIG. 6 or FIG. 7.
2. The method of claim 1, wherein the patterning of the ITO further comprises:
obtaining an ITO-coated substrate;
patterning the ITO using photolithography;
etching the ITO; and
cleaning the etched ITO and substrate.
3. The method of claim 2, wherein the ITO photolithography pattern is sprayed onto the substrate using a custom made spray mask.
4. The method of claim 1, wherein the etching of the ITO is performed with a mixed solution of HCl and HNO3.
5. The method of claim 2, wherein the etched ITO and substrate is cleaned by sonification in trichloroethylene, acetone, and isopropanol.
6. The method of claim 5, wherein the cleaning is performed at 50\xb0 C. for 20 min each, followed by drying with N2.
7. The method of claim 1, wherein the active layer solution is prepared my mixing P3HT and PCBM with a weight ratio of 1:1 in dichlorobenzene.
8. The method of claim 7, wherein the active layer is stirred on a hotplate for 48 h at 60\xb0 C. prior to spraying.
9. The method of claim 1, wherein the layer comprising poly (3,4) ethylenedioxythiophene:poly-styrenesulfonate mixed with 5 vol. % of dimethylsulfoxide is prepared by:
diluting the poly (3,4) ethylenedioxythiophene:poly-styrenesulfonate
filtering the diluted poly (3,4) ethylenedioxythiophene:poly-styrenesulfonate through a 0.45 \u03bcm filter; and
mixing the dimethylsulfoxide into the diluted poly (3,4) ethylenedioxythiophene:poly-styrenesulfonate.
10. The method of claim 1, wherein the high vacuum is 10\u22126 Torr.
11. The method of claim 1, further comprising assembling the organic solar photovoltaic cell into an array of 50 individual cells with an active area of 12 mm2.
12. The method of claim 10, wherein the array is configured with 10 cells in series, in one row, and 5 rows in parallel.
13. The method of claim 1, wherein the Self Assembled Monolayer layer comprises N-propyl trimethoxysilane.