1. A method for the detection of at least one toxin in a sample, comprising the steps of:
a) providing:
i) a cell culture comprising human foreskin fibroblast cells, wherein said cell culture has been frozen and thawed in situ on a substrate, wherein the in situ frozen and thawed cells are suitable for detecting at least one toxin, and
ii) a sample suspected of containing at least one toxin;
b) adding said sample to said cell culture on said substrate without incubating said cell culture after thawing, to produce an inoculated culture;
c) incubating said inoculated culture to produce an intoxicated culture; and
d) observing said intoxicated culture for the presence of cytopathic effect on said cells of said cell culture, thereby detecting said at least one toxin.
2. The method of claim 1, wherein said substrate is selected from the group consisting of glass and plastic.
3. The method of claim 2, wherein said glass is a glass coverslip.
4. The method of claim 2, wherein said plastic substrate is the well of a multiwell plate.
5. The method of claim 1, wherein said toxin is a Clostridium toxin.
6. The method of claim 1, wherein said toxin is a Clostridium difficile toxin.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of geometric deformation of a seismic image for interpretation, comprising:
selecting a geologic layer represented in the seismic image;
determining by stratigraphic modelling a mean topographic depositional surface for said layer;
deforming the seismic image by displacing the traces by taking account of the modelled mean depositional surface; and
carrying out a geologic interpretation of the deformed image
2. A method as claimed in claim 1, wherein said stratigraphic modelling is based on a principle of monolithologic stationary diffusion.
3. A method as claimed in claim 1 wherein, the layer is defined by a top and a base thereof, the top being adjusted on the mean surface.
4. A method as claimed in claim 1 wherein, the layer is defined by a top and a base thereof with, the base being adjusted on the mean surface
5. A method as claimed in claim 2, wherein the layer is defined by a top and a base thereof with the top being adjusted on the mean surface.
6. A method as claimed in claim 2, wherein the layer is defined by a top and a base thereof with the base being adjusted on the mean surface.