1.-14. (canceled)
15. A method for inducing angiogenesis, which comprises administering a recombinant gelatin having an amino acid sequence derived from a partial amino acid sequence of collagen to a subject in need of angiogenesis induction.
16. (canceled)
17. The method according to claim 15, wherein the recombinant gelatin comprises repeat of a sequence represented by Gly-X-Y characteristic to collagen and has a molecular weight of 2 KDa to 100 KDa, wherein X and Y each independently represents any amino acid and a plurality of Gly-X-Y sequences may be the same or different.
18. The method according to claim 15, wherein the recombinant gelatin comprises repeat of a sequence represented by Gly-X-Y characteristic to collagen and has a molecular weight of 10 KDa to 90 KDa, wherein X and Y each independently represents any amino acid and a plurality of Gly-X-Y sequences may be the same or different.
19. The method according to claim 15, wherein the recombinant gelatin comprises repeat of a sequence represented by Gly-X-Y characteristic to collagen and a single molecule of the recombinant gelatin has at least two cell adhesion signal sequences wherein X and Y each independently represents any amino acid and a plurality of Gly-X-Y sequences may be the same or different.
20. The method according to claim 19, wherein the cell adhesion signal sequence is an amino acid sequence represented by Arg-Gly-Asp.
21. The method according to any one of claim 15, wherein the amino acid sequence of the recombinant gelatin does not comprise any of serine and threonine.
22. The method according to claim 15, wherein the amino acid sequence of the recombinant gelatin does not comprise any of serine, threonine, asparagine, tyrosine, and cysteine.
23. The method according to claim 15, wherein the amino acid sequence of the recombinant gelatin does not comprise an amino acid sequence represented by Asp-Arg-Gly-Asp.
24. The method according to claim 15, wherein the recombinant gelatin is represented by the following formula:
A-(Gly-X-Y)nm-B
wherein A represents any amino acid or amino acid sequence, B represents any amino acid or amino acid sequence, there exist n amino acids each independently represented by X, there exist n amino acids each independently represented by Y, n represents an integer from 3 to 100, m represents an integer of 2 to 10, and n Gly-X-Y sequences may be the same or different.
25. The method according to claim 15, wherein the recombinant gelatin is represented by the following formula:
Gly-Ala-Pro-(Gly-X-Y)633-Gly
wherein there exist 63 amino acids each independently represented by X, there exist 63 amino acids each independently represented by Y, and n Gly-X-Y sequences may be the same or different.
26. The method according to claim 15, wherein the recombinant gelatin has the following (1) or (2):
(1) the amino acid sequence shown in SEQ ID NO: 1; or
(2) an amino acid sequence having 80% or more homology to the amino acid sequence shown in SEQ ID NO: 1, and having an angiogenic action.
27. The method according to claim 15, wherein the recombinant gelatin is crosslinked.
28. The method according to claim 27, wherein crosslinking is carried out using an aldehyde, condensing agent, or enzyme.
29. The method according to claim 15, wherein the recombinant gelatin accumulates at an angiogenesis site so as to induce angiogenesis.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1.-12. (canceled)
13. A method for determining signal propagation delay between a first station and a second station, comprising:
generating a first series of first signal pulses in the first station at a first pulse-repetition rate f1;
generating a second series of second signal pulses in the second station at a second pulse-repetition rate f2 and generating a third series of third signal pulses in the second station at a third pulse-repetition rate f3, with f2=f1+\u0394f and f3=f1\u2212\u0394f applying to the pulse-repetition rates in the second station;
transmitting, by the second station, the second series of second signal pulses and the third series of third signal pulses to the first station for receipt at the first station;
feeding the second series of second signal pulses and the third series of third signal pulses received at the first station to a correlator and correlating the second series of second signal pulses and the third series of third signal pulses in the correlator with the first series of first signal pulses generated in the first station to generate a resultant series of result signal pulses; and
determining the signal propagation delay from a time distance \u0394 between two successive signal pulses of the resultant series of resultant signal pulses.
14. The method as claimed in claim 13, wherein a signal pulse of the resultant series of resultant signal pulses is generated in the correlator whenever a signal pulse of the first series of first signal pulses coincides in time with a signal pulse of the received second series of second signal pulses and the third series of third signal pulses.
15. The method as claimed in claim 13, wherein the second series of second signal pulses and the third series of third signal pulses in the second station are first mutually superimposed to form a further series by adding the second and third series; and wherein the second and third series are subsequently transmitted to the first station as a further series of signal pulses and received at the first station.
16. The method as claimed in claim 14, wherein the second series of second signal pulses and the third series of third signal pulses in the second station are first mutually superimposed to form a further series by adding the second and third series; and wherein the second and third series are subsequently transmitted to the first station as a further series of signal pulses and received at the first station.
17. The method as claimed in claim 13, wherein the second series of second signal pulses and the third series of third signal pulses are fed to a switch in the second station before being transmitted to the first station, the switch being controlled to alternately select one of the second series of second signal pulses and the third series of third signal pulses to form a selected series, and the selected series being transmitted to the first station and received by the first station.
18. The method as claimed in claim 14, wherein the second series of second signal pulses and the third series of third signal pulses are fed to a switch in the second station before being transmitted to the first station, the switch being controlled to alternately select one of the second series of second signal pulses and the third series of third signal pulses to form a selected series, and the selected series being transmitted to the first station and received by the first station.
19. The method as claimed in claim 17, wherein the switch is driven to change over at an instant in which the second series of second signal pulses and the third series of third signal pulses are generated simultaneously.
20. The method as claimed, in claim 13, wherein the second series of second signal pulses and third series of third signal pulses are generated with a fixed mutual phase relationship.
21. The method as claimed in claim 13, wherein the second pulse-repetition rate f2 and the third pulse-repetition rate f3 are controlled by a phase-locked loop of the second station.
22. The method as claimed in claim 21, wherein at least one of the second series of second signal pulses is generated by a pulse generator controlled by the phase-locked loop such that the second series has second pulse-repetition rate f2 and the third series of third signal pulses is generated by the pulse generator controlled by the phase-locked loop such that the third series has third pulse-repetition rate f3
23. The method as claimed in claim 21, wherein the first series of first signal pulses is transmitted by the first station to the second station and received at the second station;
wherein the first series of signal pulses received at the second station is fed to a correlator of the second station at which the received first series of signal pulses is correlated with one of the second series of second pulses signal and the third series of third pulse signals to generate a signal; and
wherein the signal is fed to the phase-locked loop and the phase-locked loop subsequently in turn makes the second pulse-repetition rate f2 and third pulse-repetition rate f3 available based on the generated signal.
24. The method as claimed in claim 22, wherein the first series of first signal pulses is transmitted by the first station to the second station and received at the second station;
wherein the first series of signal pulses received at the second station is fed to a correlator of the second station at which the received first series of signal pulses is correlated with one of the second series of second pulses signal and the third series of third pulse signals to generate a signal; and
wherein the signal is fed to the phase-locked loop and the phase-locked loop subsequently in turn makes the second pulse-repetition rate f2 and third pulse-repetition rate f3 available based on the generated signal.
25. The method as claimed in claim 13, wherein the a correlator comprises a mixer.
26. An arrangement for determining signal propagation delay \u03c4 between a first station in the arrangement and a second station in the arrangement, comprising a first pulse generator arranged in the first station, the first pulse generator generating a first series of first signal pulses at a first pulse-repetition rate f1;
at least one further pulse generator arranged in the second station, the at least one further pulse generator generating a second series of second signal pulses at a second pulse-repetition rate f2 and a third series of third signal pulses at a third pulse-repetition rate f3, with f2=f1+\u0394f and f3=f1\u2212\u0394f applying to the pulse-repetition rates;
a second transmitting device arranged in the second station and for transmitting the second series of second pulse signals and the third series of third pulse signals to the first station;
a receiving device and a correlator arranged in the first station, the receiving device receiving the transmitted second series of second pulse signals and the third series of third pulse signals and the correlator correlating the received second series of second pulse signals and the third series of third pulse signals with the first series to generate a resultant series of signal pulses; and
an evaluation unit arranged in the first station, the evaluation unit determining a time distance between two successive signal pulses of the resultant series of signal pulses.
27. The arrangement as claimed in claim 26, wherein the second station includes a switch connected to the at least one further pulse generator and to the transmitting device, the switch being configured to select one of the second series of second signal pulses and the third series of third signal pulses for transmittal to the first station.
28. The arrangement as claimed in claim 26, wherein the first station includes a transmitting device for transmitting the first series of first signal pulses to the second station;
wherein the second station includes a receiving device for receiving the transmitted first series of signal pulses and a correlator for correlating the received first series of signal pulses with at least one of the second series of second signal pulses and the third series of third signal pulses, with a signal being generated during the correlation; and
wherein the second station includes a phase-locked loop to which the signal generated during the correlation is fed, the phase-locked loop controlling the at least one further pulse generator based on the signal generated during the correlation.
29. The arrangement as claimed in claim 27, wherein the first station includes a transmitting device for transmitting the first series of first signal pulses to the second station
wherein the second station includes a receiving device for receiving the transmitted first series of signal pulses and a correlator for correlating the received first series of signal pulses with at least one of the second series of second signal pulses and the third series of third signal pulses, with a signal being generated during the correlation; and
wherein the second station includes a phase-locked loop to which the signal generated during the correlation is fed, the phase-locked loop controlling the at least one further pulse generator based on the signal generated during the correlation.