What is claimed is:
1. A composition comprising a purified antimicrobial polypeptide and hydroxyethyl starch.
2. The composition of claim 1, wherein said purified antimicrobial polypeptide and said hydroxyethyl starch are in solution.
3. The composition of claim 1, wherein said purified antimicrobial polypeptide is a purified defensin.
4. The composition of claim 2, wherein said purified defensin is present in a concentration of about 0.01 to 1000 mgl.
5. The composition of claim 2, wherein said purified defensin is present in a concentration of about 0.1 to 5 mgl.
6. The Composition of claim 2, wherein said hydroxyethyl starch is present in a concentration of about 1 to 200 gl.
7. The composition of claim 2, wherein said purified defensin is present in a concentration of about 0.01 to 1000 mgl and said hydroxyethyl starch is present in a concentration of about 1 to 200 gl.
8. The composition of claim 3, wherein said defensin is selected from the group consisting of SEQ ID NOs: 37-95.
9. The composition of claim 3, wherein said defensin is encoded by SEQ ID NO:37.
10. The composition of claim 1, further comprising a cell surface receptor binding compound.
11. The composition of claim 10, wherein said cell surface receptor binding compound is selected from the group consisting of IGF-1, EGF, NGF, and substance P and combinations thereof.
12. A composition comprising an antimicrobial polypeptide and an impermeant anion selected from the group consisting of lactobionate and gluconate.
13. The composition of claim 12, wherein said antimicrobial polypeptide and said impermeant ion are in solution.
14. The composition of claim 12, wherein said antimicrobial polypeptide is a purified defensin.
15. The composition of claim 14, wherein said purified defensin is present in a concentration of about 0.01 to 1000 mgl.
16. The composition of claim 14, wherein said impermeant ion is lactobionic acid, and wherein said lactobionate is present in a concentration of about 1 to 500 mM.
17. The composition if claim 14, wherein said impermeant anion is gluconate, and wherein said mannitol is present in a concentration of about 1 to 500 mM.
18. The composition of claim 14, wherein said defensin is selected from the group consisting of SEQ ID NOs: 37-95.
19. The composition of claim 14, wherein said defensin is encoded by SEQ ID NO:37.
20. The composition of claim 14, further comprising a cell surface receptor binding compound.
21. The composition of claim 20, wherein said cell surface receptor binding compound is selected from the group consisting of IGF-1, EGF, NGF, and substance P and combinations thereof.
22. A composition comprising a purified antimicrobial polypeptide and an ex vivo internal organ.
23. The composition of claim 22, wherein said purified antimicrobial polypeptide is in solution at a concentration of about 0.01 to 1000 mgl.
24. The composition of claim 22, wherein said ex vivo internal organ is selected from kidneys, hearts, lungs, small intestines, large intestines, livers, and pancreases.
25. The composition of claim 22, wherein said antimicrobial polypeptide is encoded by SEQ ID NO:37.
26. The composition of claim 22, further comprising a macromolecular oncotic agent selected from the group consisting of hydroxyethyl starch, dextran, and glucose.
27. The composition of claim 22, further comprising an impermeant anion selected from the group consisting of gluconate and lactobionate.
28. The composition of claim 22, further comprising glutathione.
29. The composition of claim 22, further comprising a cell surface receptor binding compound.
30. The composition of claim 22, wherein said cell surface receptor binding compound is selected from the group consisting of IGF-1, EGF, NGF, and substance P.
31. A method comprising:
a) providing:
i) cellular material selected from the group consisting of internal organs, skin, and gametes; and
ii) a solution comprising a purified antimicrobial polypeptide;
b) storing said cellular material in said solution comprising a purified antimicrobial peptide.
32. The method of claim 31, wherein said purified antimicrobial polypeptide is in solution at a concentration of about 0.01 to 1000 mgI.
33. The method of claim 31, wherein said cellular material is an internal organ.
34. The method of claim 33, wherein said internal organ is infused with said solution.
35. The method of claim 34, wherein said internal organ is selected from the group consisting of kidneys, hearts, lungs, small intestines, large intestines, livers, and pancreases.
36. The method of claim 33, wherein said internal organ is a human organ.
37. The method of claim 31, wherein said purified antimicrobial polypeptide is a defensin selected from the group consisting of SEQ ID Nos: 37-95.
38. The method of claim 31, wherein said antimicrobial polypeptide is encoded by SEQ ID NO:37.
39. The method of claim 31, wherein said solution further comprises a macromolecular oncotic agent selected from the group consisting of hydroxyethyl starch, dextran, and glucose.
40. The method of claim 31, wherein said solution further comprises an impermeant anion selected from the group consisting of gluconate and lactobionate.
41. The method of claim 31, wherein said solution further comprises a cell surface receptor binding compound.
42. The method of claim 41, wherein said cell surface receptor binding compound is selected from the group consisting of IGF-1, EGF, NGF, and substance P and combinations thereof.
43. A composition comprising a cell surface receptor binding compound and hydroxyethyl starch.
44. The composition of claim 43, wherein said cell surface receptor binding compound and said hydroxyethyl starch are in solution.
45. The composition of claim 44, wherein said cell surface receptor binding compound is selected from the group consisting of IGF-1, EGF, NGF, and substance P and combinations thereof.
46. A kit comprising:
a) a vessel containing a solution comprising a compound selected from the group consisting of lactoboinate and hydroxyethyl starch; and
b) a vessel containing an antimicrobial polypeptide.
47. The kit of claim 46, wherein said antimicrobial polypeptide is BNP-1.
48. The kit of claim 46, wherein said vessel containing an antimicrobial polypeptide further comprises a cell surface receptor binding compound.
49. The kit of claim 48, wherein said cell surface receptor binding compound is selected from the group consisting of IGF-1, EGF, NGF, and substance P.
50. The kit of claim 46, further comprising instructions for combining said solution and said antimicrobial polypeptide to form an organ storage solution.
51. A process for producing a storage solution comprising:
a) providing a solution comprising a compound selected from the group consisting of hydroxyethyl starch and lactobionate and a purified antimicrobial polypeptide; and
b) combining said solution with said purified antimicrobial polypeptide to form a storgae solution.
52. The process of claim 51, further comprising the steps of providing at least one cell surface receptor binding compound and combining said at least one cell surface receptor binding compound with said solution and said antimicrobial polypeptide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A sheet conveying apparatus comprising:
an apparatus body including a sheet conveying path;
a conveying roller pair including a first roller provided on one side with respect to the sheet conveying path and a second roller provided on another side with respect to the sheet conveying path, the conveying roller pair conveying the sheet along the sheet conveying path by nipping the sheet by a nip portion between the first and second rollers;
a rotary member provided on the second roller side with respect to the sheet conveying path, the rotary member including:
a first abutment portion against which a front end in the conveying direction of a first sheet abuts in a state in which the rotary member is positioned at a first abutment position, the rotary member being rotated in a predetermined direction from the first abutment position by the first abutment portion being pushed by the first sheet; and
a second abutment portion against which a front end in the conveying direction of a second sheet conveyed following the first sheet abuts in a state in which the rotary member is positioned at a second abutment position rotated in the predetermined direction from the first abutment position, the rotary member being rotated in the predetermined direction from the second abutment position by the second abutment portion being pushed by the second sheet; and
a restricting portion provided on the second roller side with respect to the sheet conveying path and near the nip portion, the restricting portion being configured such that the first abutment portion is positioned on the second roller side from a straight line connecting a point protruding most to the sheet conveying path of the restricting portion and the nip portion in a state in which the second abutment portion is in contact with a surface of the first sheet as the rotary member rotates in the predetermined direction by the first abutment portion being pushed by the first sheet.
2. The sheet conveying apparatus according to claim 1, further comprising a downstream guide guiding the sheet conveyed by the nip portion on the side downstream in the conveying direction of the nip portion,
wherein the restricting portion composes at least a part of the downstream guide.
3. The sheet conveying apparatus according to claim 1, wherein the downstream guide includes a guide roller guiding the sheet that has passed through the nip portion along the sheet conveying path and the restricting portion composes a point protruding most to the sheet conveying path by the guide roller.
4. The sheet conveying apparatus according to claim 2, wherein the apparatus body is configured to permit to access to the sheet conveying path from an outside of the apparatus body on the side downstream in the conveying direction of the nip portion.
5. The sheet conveying apparatus according to claim 4, wherein the apparatus body is configured such that an image forming unit having an image forming portion configured to form an image on the sheet conveyed by the conveying roller pair is detachably mounted above, and
the downstream guide is opened to permit to connect to a conveying guide on the image forming unit side at an upper surface of the apparatus body.
6. The sheet conveying apparatus according to claim 1, wherein the rotary member is provided coaxially with the second roller.
7. The sheet conveying apparatus according to claim 1, wherein the rotary member corrects a skew of a sheet conveyed to the nip portion by causing the first and second abutment portions abut against the sheet on the side upstream in the conveying direction of the nip portion of the conveying roller pair at the first and second abutment positions.
8. The sheet conveying apparatus according to claim 1, further comprising a positioning mechanism positioning the rotary member to the first and second abutment positions.
9. The sheet conveying apparatus according to claim 8, wherein the positioning mechanism is configured to position the rotary member at the second abutment position by rotating the rotary member in the predetermined direction after the rotary member starting to be rotated in the predetermined direction by the first abutment portion being pushed by the first sheet.
10. The sheet conveying apparatus according to claim 8, wherein the positioning mechanism includes a cam member, a cam follower contacting with the cam member and an elastic member biasing the cam follower toward the cam member.
11. The sheet conveying apparatus according to claim 1, further comprising a detecting portion configured to detect a rotation of the rotary member.
12. A sheet conveying apparatus comprising:
an apparatus body;
a conveying roller pair including a first roller provided on one side with respect to a sheet conveying path and a second roller provided on another side with respect to the sheet conveying path, the conveying roller pair conveying the sheet along the sheet conveying path by nipping the sheet by a nip portion between the first and second roller;
a rotary member provided on the second roller side with respect to the sheet conveying path, the rotary member including:
a first abutment portion against which a front end in the conveying direction of a first sheet abuts in a state in which the rotary member is positioned at a first abutment position, the rotary member being rotated in a predetermined direction from the first abutment position by the first abutment portion being pushed by the first sheet; and
a second abutment portion against which a front end in the conveying direction of a second sheet conveyed following the first sheet abuts in a state in which the rotary member is positioned at a second abutment position rotated in the predetermined direction from the first abutment position, the rotary member being rotated in the predetermined direction from the second abutment position by the second abutment portion being pushed by the second sheet;
an upstream guide configured to guide the sheet from the upstream side in the conveying direction toward the nip portion along the sheet conveying path; and
a downstream guide guiding the sheet being nipped and conveyed by the nip portion of the conveying roller pair on the side downstream in the conveying direction of the nip portion, the downstream guide having a restricting portion provided on the second roller side with respect to the sheet conveying path and protruding to the first roller side, and the restricting portion being configured to protrude such that the second abutment portion is positioned on the second roller side rather than a guide surface of the first roller side of the upstream guide in a state in which a front end portion of the first abutment portion is positioned on a straight line connecting a point protruding most to the sheet conveying path of the restricting portion and the nip portion.
13. The sheet conveying apparatus according to claim 12, wherein the restricting portion is composed of a guide roller guiding a sheet that has passed through the nip portion along the sheet conveying path.
14. The sheet conveying apparatus according to claim 12, wherein the apparatus body is configured such that an image forming unit having an image forming portion configured to form an image on the sheet conveyed by the conveying roller pair is detachably mounted above, and
the downstream guide is opened to permit to connect to a conveying guide on the image forming unit side at an upper surface of the apparatus body.
15. An image forming apparatus comprising:
the sheet conveying apparatus as set forth in claim 1; and
an image forming portion configured to form an image on a sheet conveyed by the sheet conveying apparatus.
16. The image forming apparatus according to claim 15 further comprising an image forming apparatus body including the image forming portion,
wherein the sheet conveying apparatus is detachably mounted with the image forming apparatus body and to permit to access to the sheet conveying path from an outside of the apparatus in a state in which the sheet conveying apparatus is detached from the image forming apparatus body.