1. A method comprising:
(1) providing a set of primers that generate targets from genomic regions which include a polymorphic locus of interest;
(ii) providing an expanded probe set comprising a core subset of probes that hybridize only to particular targets with particular alleles and a second subset of probes that hybridize to multiple targets;
(iii) exposing the targets to the expanded probe set under reaction conditions such that hybridization of targets and probes generates a signal;
(iv) performing allele group assignments based on the signal of hybridization of targets and members of the core subset of probes.
2. The method of claim 1, further comprising performing allele group assignments based on the signal of hybridization of targets and the second subset of probes.
3. The method of claim 2, further comprising making final allele assignments where allele group assignments made based on the second probe set agree with allele group assignments made based on the first subset of probes.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A solid particle dispenser comprising:
a particle container having an upper closure wall, side closure wall, and a lower closure wall;
said upper closure wall having a shaped rotation preventing opening;
said lower closure wall having a plurality of open passages essentially uniformly spaced therethrough;
a helical coil spring having an upper extent and a lower extent within said dispenser;
a shaped plunger for passing through said shaped rotation preventing opening;
said upper extent of said helical spring is secured to said shaped plunger that passes through said upper closure wall shaped rotation preventing opening;
a movable plate extending over said lower closure wall with apertures essentially uniformly spaced therethrough;
said lower extent of said helical coil spring is secured to said movable apertured plate such that pressing said shaped plunger inwardly rotates said movable apertured plate to aline said movable apertured plate apertures with said lower closure wall open passages for essentially uniformly dispensing particles.
2. A solid particle dispenser as in claim 1 wherein:
aid upper extent of said helical coil spring has a smaller radial diameter than said lower extent of said helical coil spring such that pressing on said plunger moves said upper extent of said helical coil spring vertically to rotate said lower extent of said helical coil spring within said dispenser.
3. A solid particle dispenser as in claim 2 wherein:
said helical coil spring coils uniformly increase in radial diameter from said upper extent to said lower extent of said helical coil spring.
4. A solid particle dispenser as in claim 1 wherein:
said movable apertured plate apertures are elongated to increase the distance said lower closure wall open passages are exposed to particles within said dispenser as said movable apertured plate is rotated.
5. A solid particle dispenser as in claim 4 wherein:
said movable apertured plate elongated open apertures and said lower closure wall open passages are positioned to be alined and dispense particles both when said plunger is depressed and when said plunger is raised by return pressure from said helical coil spring, to dispense particles on both forward and return rotation of said movable apertured plate.
6. A solid particle dispenser as in claim 1 wherein:
said dispenser having means to limit rotation of said movable apertured plate.
7. A solid particle dispenser as in claim 6 wherein:
said means to limit movement of said movable apertured plate is a limit stop on said lower closure wall that extends into a limit slot in said movable apetured plate.
8. A solid particle dispenser as in claim 6 wherein:
said means to limit movement of said movable apertured plate is a tab on said movable apertured plate that abuts against an adjustable stop in a recess associated with said side closure wall.
9. A solid particle dispenser as in claim 8 wherein:
said adjustable stop projects through an adjustment slot in said side closure wall.
10. A solid particle dispenser as in claim 9 wherein:
said adjustable stop is attached to a stop stem on an adjustment slide.
11. A solid particle dispenser as in claim 6 wherein:
said means to limit rotation of said movable apertured plate has an adjustable stop associated with said side closure wall;
a peripheral slot is in said movable apertured plate;
said adjustable stop protrudes into said peripheral slot to limit movement of said movable apertured plate.
12. A solid particle dispenser as in claim 11 wherein:
said adjustable stop is attached to an adjustment slide by a stop stem;
an adjustment slot is provided in said side closure wall for passage of said stop stem.
said adjustable stop and said stop stem extend through said adjustment slot and frictionally hold said adjustable stop in said side closure wall.
13. A solid particle dispenser as in claim 6 wherein:
said means to limit movement of said movable apertured plate is an adjustable abutting stopper on said shaped plunger.
14. A solid particle dispenser as in claim 13 wherein:
said shaped plunger has a longitudinal slot facing outwardly;
said abutting stopper is adjusted by frictionally sliding said abutting stopper within said longitudinal slot in said shaped plunger.
15. A solid particle dispenser as in claim 6 including:
a plunger flange below and attached to said shaped plunger to limit movement of said shaped plunger.
16. A solid particle dispenser as in claim 15 wherein:
said means to limit movement of said movable apertured plate is a cylinder positioned under said plunger flange.
17. A solid particle dispenser as in claim 16 wherein:
said cylinder is provided with different depth recesses to adjust the distance said shaped plunger can be depressed into said dispenser.
18. A solid particle dispenser as in claim 17 wherein:
said cylinder is rotatable to selectively place different depth recesses under said plunger flange.
19. A solid particle dispenser as in claim 18 including:
a depth selection dial attached to said cylinder for rotating said cylinder;
indicia on said upper closure wall for alinement with said depth selection dial to indicate said recessed depth of said selected recess.