1.-26. (canceled)
27. An isolated glutamine endopeptidase enzyme that cleaves a peptide bond after a QPF and a PFP motif in glutens.
28. The isolated glutamine endopeptidase enzyme of claim 27 that also cleaves a peptide bond after an XPY motif in glutens.
29. The isolated glutamine endopeptidase enzyme of claim 27 that also cleaves a peptide bond after an XPQ motif in glutens.
30. The isolated glutamine endopeptidase enzyme of claim 27 that also cleaves a peptide bond after both an XPQ and an XPY motif in glutens.
31. The isolated glutamine endopeptidase enzyme of claim 27 that is in the form of a formulation.
32. The formulation of claim 31, wherein the formulation is added to a gluten containing food stuff prior to consumption of the gluten-containing food stuff.
33. The formulation of claim 31, wherein the formulation is added in vivo concurrent with or after consumption of a gluten-containing food stuff.
34. The formulation of claim 32, wherein the formulation is admixed to the gluten-containing food stuff.
35. The formulation of claim 31, wherein the isolated enzyme has an apparent molecular weight of about 70-75 kDa as determined by gliadin zymograms or by sodium dodecyl sulfate polyacrylamide gel electrophoresis.
36. The formulation of claim 35, wherein the isolated enzyme has a functional pH range of 3-10 as determined by detectable Z-YPQ-pNA cleaving activity within a 72 hour digestion period and a functional pH range of 7-10 as determined by substantially complete Z-YPQ-pNA cleavage within a 1 hour digestion period.
37. The formulation of claim 36, wherein the isolated enzyme is 100% inhibited by 1 mM of EDTA or PMSF.
38. The formulation of claim 37, wherein the isolated enzyme is derived from a Rothia species bacteria, wherein the Rothia species bacteria is selected from the group consisting of Rothia mucilaginosa ot 681 (strain WSA-2B), Rothia mucilaginosa ATCC 25296 and Rothia species ot 188 (strain WSA-8).
39. The formulation of claim 38, wherein the isolated enzyme is stable in acid conditions.
40. The formulation of claim 39, wherein the isolated enzyme is lyophilized.
41. The formulation of claim 40, wherein the isolated enzyme has an amino acid sequence that show at least 60% similarity to SEQ. ID. NO: 1.
42. The formulation of claim 40, wherein the isolated enzyme comprises SEQ. ID. NO: 1.
43. The formulation of claim 40, wherein the isolated enzyme consists essentially of SEQ. ID. NO: 1.
44. The formulation of claim 40, wherein the isolated enzyme is SEQ. ID. NO: 1.
45. The formulation of claim 44 further comprising a prolyl endopeptidase.
46. A method of digesting gluten, the method comprising contacting gluten-containing foodstuff with an effective dose of a formulation of claim 42.
47. The method of claim 46, wherein the contacting is performed in vitro prior to consumption of the gluten-containing food stuff.
48. The method of claim 46, wherein the contacting is performed in vivo concurrent with or after consumption of the gluten-containing food stuff.
49. An assay for diagnosing Celiac Sprue, gluten allergy andor dermatitis herpetiformis in a subject comprising
a) contacting a biological sample from the subject with a fixed amount of gliadin for a 24 hour period;
b) measuring the amount of gliadin digestion; and
c) comparing the amount of gliadin digestion for the biological sample with that obtained for a control assay, wherein the control assay is a mixture of a same fixed amount of gliadin with a formulation of claim 46 for a 24 hour period, wherein if the extent of gliadin digestion of less than 50% of that of the control assay indicates the subject likely have Celiac Sprue, gluten allergy andor dermatitis herpetiformis.
50. The assay of claim 49, wherein the subject is at risk of developing Celiac Sprue, gluten allergy andor dermatitis herpetiformis.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method, comprising:
loading and displaying (240) a guideline graph based on a first user input;
receiving (260) a second user input selecting a desired portion of the guideline graph; and
processing the second user input to load and display (270) a sub-graph including the desired portion of the guideline graph.
2. The method of claim 1, wherein the guideline graph and the sub-graph include a plurality of nodes, each of the nodes representing a task to be completed by a user.
3. The method of claim 2, wherein each of the nodes is represented by at least a workflow step class and a graph node class, wherein the workflow step class represents shared properties of the node such that all nodes overlap and are synchronized between all guideline graphs and sub-graphs viewable over a network, and wherein the graph node class represents graph-specific properties.
4. The method of claim 2, wherein a portion of the plurality of nodes includes a visual feature to indicate one of (i) portions of the guideline graph that are viewable as a sub-graph and (ii) a status of a node, the status of the node being one of a level of completion of a task.
5. The method of claim 4, wherein the visual feature includes one of a shape, a size, a color and a font type.
6. The method of claim 1, wherein the first user input indicates at least one of a user type and a disease.
7. The method of claim 1, further comprising:
receiving (250, 280) a third user input including one of an information to update one of the guideline graph and the sub-graph and a selection of another viewing option.
8. The method of claim 1, further comprising:
determining a time remaining before a task from the sub-graph should be completed and a time elapsed since a patient event and displaying one of the time remaining and the time elapsed in a timer node.
9. The method of claim 8, further comprising:
generating an alert alerting a user that the task is due to be completed.
10. The method of claim 8, wherein the timer node includes a bar graph showing the time remaining and the time elapsed.
11. The method of claim 2, wherein each of the nodes is one of connected to another node via an arrow to indicate an order of the tasks and free-floating to indicate that the task is not required to precede and follow another task.
12. A system, comprising:
a user interface (104) to enter a first and a second user input;
a memory (108);
a processor (102) loading the guideline graph from the memory (108) based on the first user input and a sub-graph based on the second user input, which selects a portion of the guideline graph that a user desires to view in the sub-graph; and
a display (106) displaying the guideline graph and the sub-graph.
13. The system of claim 12, wherein the guideline graph and the sub-graph include a plurality of nodes, each of the nodes representing a task to be completed by a user.
14. The system of claim 13, wherein each of the nodes is represented by at least a workflow step class and a graph node class, wherein the workflow step class represents shared properties of the node such that all nodes overlap and are synchronized between all guideline graphs and sub-graphs viewable over a network, and wherein the graph node class represents graph-specific properties.
15. The system of claim 13, wherein a portion of the plurality of nodes includes a visual feature to indicate one of (i) portions of the guideline graph that are viewable as a sub-graph and (ii) a status of a node, the status of the node being one of a level of completion of a task.
16. The system of claim 15, wherein the visual feature includes one of a shape, a size, a color and a font type.
17. The system of claim 12, wherein the first user input indicates at least one of a user type and a disease.
18. The system of claim 12, wherein a third user input is entered via the user interface, the third user input including one of an information to update one of the guideline graph and the sub-graph and a selection of another viewing option.
19. The system of claim 12, wherein the processor (102) determines a time remaining before a task from the sub-graph should be completed and a time elapsed since a patient event and displaying one of the time remaining and the time elapsed in a timer node.
20. The system of claim 19, wherein the processor (102) generates an alert alerting a user that the task is due to be completed.
21. The system of claim 19, wherein the timer node includes a bar graph showing the time remaining and the time elapsed.
22. The system of claim 13, wherein each of the nodes is one of connected to another node via an arrow to indicate an order of the tasks and free-floating to indicate that the task is not required to precede and follow another task.
23. A computer-readable storage medium (106) including a set of instructions executable by a processor (102), the set of instructions operable to:
load and display (240) a guideline graph based on a first user input;
receive (260) a second user input selecting a desired portion of the guideline graph; and
process the second user input to load and display (270) a sub-graph including the desired portion of the guideline graph.