1. An isolated nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising a nucleotide sequence that encodes SEQ ID NO: 40;
b) a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleic acid molecule that encodes the peptide of SEQ ID NO: 40;
c) a nucleic acid molecule comprising a nucleotide sequence that encodes a peptide that is at least 95% identical to the amino acid sequence of SEQ ID NO: 40; and
d) a nucleic acid molecule comprising a nucleotide sequence that hybridizes with a full-length complement of a nucleotide sequence that encodes the peptide of SEQ ID NO: 40, wherein hybridization conditions comprise two washes in 0.2\xd7SSC at 65\xb0 C. for 20 minutes each; and
wherein each of the peptides encoded by the nucleic acid molecule of a), b), c) or d) targets a translationally fused polypeptide into plant plastids.
2. An isolated nucleic acid molecule encoding a fusion polypeptide comprising the nucleic acid molecule of claim 1.
3. A vector comprising the nucleic acid molecule of claim 2.
4. A plant cell which comprises the vector of claim 3.
5. A transgenic plant comprising the nucleic acid molecule of claim 2.
6. The transgenic plant of claim 5, wherein the plant is selected from the group consisting of maize, soybean, tomato, potato, cotton, sunflower, alfalfa, lettuce, tobacco, and rice.
7. A method for targeting a polypeptide to a plastid in a plant comprising introducing into the plant a vector comprising a first nucleic acid molecule encoding a plastid peptide linked to a second nucleic acid molecule encoding said polypeptide such that translation of the first and second nucleic acid molecule produces a fusion protein, wherein said first nucleic acid molecule is the nucleic acid molecule of claim 1.
8. The method of claim 7 wherein the plastid transit peptide is N-terminal to the polypeptide in the fusion protein.
9. The method of claim 7 wherein the polypeptide is selected from the group consisting of Bt toxin proteins, EPSP synthase, GAT, ALS, and enzymes that modify a physiological process that occurs in a plastid.
10. The method of claim 9 wherein the physiological process is photosynthesis, fatty acid synthesis, amino acid synthesis, oil synthesis, carotenoid synthesis, terpenoid synthesis, and starch synthesis.
11. The method of claim 7 wherein the polypeptide is isolated from the plant plastids.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A decorative architectural ornament and bracket, said decorative architectural ornament comprising a gripping slot defined by two side walls;
said bracket comprising two arms;
wherein the first arm defines mounting holes;
wherein the second arm defines a gripping end;
wherein said first arm is adapted to be secured to a wall or substrate and said gripping end of said second arm is adapted to be mated with said gripping slot of said decorative architectural ornament, thereby securing said decorative architectural ornament to a wall or substrate.
2. The decorative architectural ornament and bracket of claim 1, wherein the decorative architectural ornament further comprises weep holes.
3. The decorative architectural ornament and bracket of claim 1, wherein the gripping end of the bracket defines one or more teeth.
4. A method for installing a decorative architectural ornament on a wall or substrate without puncturing said decorative architectural ornament, said method comprising:
a) providing a decorative architectural ornament which defines a gripping slot;
b) providing a bracket which includes two arms;
wherein the first arm defines mounting holes; and
wherein the second arm defines a gripping end;
c) securing the first arm of the bracket to a wall or substrate;
d) mating the gripping end of the second arm of the bracket to the gripping slot of the decorative architectural ornament.
5. The method of claim 4 further comprising applying an adhesive to either the gripping end of the second arm of the bracket or the gripping slot prior to mating the gripping end to the gripping slot of the decorative architectural ornament.
6. A bracket and non-structural architectural form, comprising:
an L-shaped bracket member having a first bracket leg and a second bracket leg, the first bracket leg adapted to be affixed to a mounting surface using mechanical fasteners;
a non-structural architectural form adapted to be mounted to a mounting surface, the form defining a cavity adapted to receive the second bracket leg in mating engagement.
7. The bracket and non-structural architectural form of claim 6, the non-structural architectural form further comprising an abutment flange, wherein the first bracket leg is adapted to be contacted by the abutment flange when the form is mounted to the bracket.
8. The bracket and form of claim 6, wherein the abutment flange and cavity are disposed in flanges which are perpendicular to each other.
9. The bracket and form of claim 6, wherein the second bracket leg comprises one or more teeth.
10. The bracket and form of claim 6, wherein the non-structural architectural form further comprises one or more weep holes.