1. A method comprising
inserting, using a computer, a digital stamp onto a user’s computer communication;
wherein said digital stamp has at least one advertiser message thereon and is an intelligent file having computer code embedded thereon, said computer code being capable of at least one of gathering and reporting data regarding use of the digital stamp; and
wherein said computer communication comprises at least one of email communication and social network web communication.
2. The method of claim 1, wherein said inserting is performed by a user using a computer to actively insert the digital stamp on the computer communication or pre-designating the digital stamp to be inserted on the computer communication.
3. The method of claim 1, wherein said inserting comprises inserting a digital stamp into a user’s email message for display in the email message; and wherein the method further comprises using a computer to send the email message to a recipient.
4. The method of claim 1, further comprising registering the user with a digital stamp provider over a data communications network using a computer.
5. The method of claim 4, wherein said registering provides user profile information to a server, which information is stored on the server and used to suggest digital stamps to the user on a computer display prior to the user inserting a digital stamp onto a computer communication.
6. A computer-implemented method comprising
receiving a request from a user-controlled computer for a digital stamp; and facilitating computer insertion of the digital stamp by the user into a computer communication for viewing by a viewer of the digital stamp, wherein said computer communication comprises at least one of email communication and social network web communication;
wherein said digital stamp has at least one advertiser message thereon and is an intelligent file having computer code embedded thereon, said computer code being capable of at least one of gathering and reporting data regarding use of the digital stamp.
7. The method of claim 6, further comprising logging, using a computer, a user’s use of a digital stamp in computer communications, wherein said use comprises a user inserting using a computer a digital stamp onto a computer communication.
8. The method of claim 7, further comprising compensating the user for inserting a digital stamp into a computer communication.
9. The method of claim 8, wherein the digital stamp further comprises at least one hyperlink to an advertiser’s website and wherein user compensation is determined at least in part based on whether the viewer clicks on a hyperlink on the digital stamp.
10. The method of claim 9, wherein user compensation is further determined based on whether the viewer makes at least one purchase from the advertiser’s website.
11. The method of claim 6, further comprising charging an advertiser for a user’s use of a digital stamp having the advertiser’s message thereon.
12. The method of claim 8, wherein said compensating comprises compensating the user with credits that may be redeemed for at least one of monetary compensation, discounts on merchandise purchases, merchandise, lottery entry, coupons from the advertiser, credit from the advertiser, and an opportunity to donate to charity.
13. The method of claim 6, further comprising providing recommended digital stamps to the user-controlled computer for display to the user prior to facilitating computer insertion of the digital stamp onto the computer communication.
14. A computer system comprising
a computer server coupled to a database,
wherein the database comprises information regarding digital stamps available for a user to include in a computer communication; and
wherein said digital stamp has at least one advertiser message thereon and is an intelligent file having computer code embedded thereon, said computer code being capable of at least one of gathering and reporting data regarding use of the digital stamp; and
wherein said computer communication comprises at least one of email communication and social network web communication.
15. The system of claim 14, wherein said information comprises at least one form of information selected from the group consisting of artwork associated with at least one digital stamp, hyperlink information, and information regarding how much a user may be compensated for using a digital stamp.
16. The system of claim 14, wherein the computer server is adapted to be able to monitor andor control usage of the digital stamps.
17. A computer-implemented method comprising
receiving input to a computer from a user requesting a digital stamp; and
facilitating computer insertion of the digital stamp by the user into a computer communication for viewing by a viewer of the digital stamp,
wherein said computer communication comprises at least one of email communication and social network web communication; and
wherein said digital stamp has at least one advertiser message thereon and is an intelligent file having computer code embedded thereon, said computer code being capable of at least one of gathering and reporting data regarding use of the digital stamp.
18. The method of claim 17, further comprising the computer communicating the digital stamp to at least a second computer having a display on which at least one viewer can view the digital stamp.
19. A computer-readable medium having computer readable instructions embodied thereon, that when read by a machine, cause the machine to perform the method of claim 6, wherein the computer readable medium is adapted to facilitate computer insertion of a digital stamp by a user.
20. A computer-readable medium having computer readable instructions embodied thereon, that when read by a machine, cause the machine to perform the method of claim 17, wherein the computer readable medium is adapted to facilitate computer insertion of a digital stamp by a user.
21. A method comprising
receiving a file to a computer, wherein the file has a graphic advertiser message thereon; and
transforming using a computer the file into an intelligent file having computer code embedded thereon, said computer code being capable of at least one of gathering and reporting data regarding use of the intelligent file, while maintaining the advertiser message on the intelligent file.
22. The method of claim 21, further comprising using said data to perform on a computer at least one of determining compensation for a user of the intelligent file, charging the advertiser for a user’s use of the intelligent file; and reporting to an advertiser demographics of users of the intelligent file.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of modulating angiogenesis in a mammal comprising administering to the mammal a therapeutically effective amount of a Nod factor.
2. The method of modulating angiogenesis in a mammal comprising administering to the mammal a therapeutically effective amount of an oligosaccharide of formula I or a pharmaceutically acceptable salt thereof:
wherein:
R1 is selected from hydrogen, \u2014X-Alk or \u2014X-Alk1-Q-Y-Alk2;
wherein:
X is selected from \u2014C(O)\u2014, \u2014C(NRN)\u2014, \u2014C(S)\u2014, \u2014SO2\u2014, \u2014P(O)(ORN)\u2014 wherein RN is hydrogen, hydroxy, amino, optionally substituted C1-8alkyl, optionally substituted C2-8alkenyl, optionally substituted C2-8alkynyl, optionally substituted and optionally substituted aryl;
Alk is selected from an optionally substituted, straight chain or branched, alkyl, alkenyl or alkynyl group having from 2 to 30 carbon atoms;
Alk1 is absent or present and is selected from an optionally substituted divalent C1-10alkyl, optionally substituted divalent C2-10alkenyl and optionally substituted divalent C2-10alkynyl chain;
Q is absent or present and is selected from an optionally substituted divalent cycloalkyl, optionally substituted divalent cycloalkenyl, optionally substituted divalent heterocycle, optionally substituted divalent aryl or optionally substituted divalent heteroaryl ring system;
Y is absent or present and is selected from \u2014NH\u2014, \u2014O\u2014, \u2014S\u2014, \u2014NHC(O)\u2014, \u2014C(O)NH\u2014, NHSO3\u2014, \u2014C(RG)\u2550N\u2014N\u2014, \u2014NHC(O)NH\u2014, \u2014NHC(S)NH\u2014, \u2014NHC(NH)NH\u2014, \u2014C(RG)\u2550N, and \u2014N\u2550C(R)\u2014, wherein RG is hydrogen, optionally substituted C1-6alkyl, optionally substituted arylC1-4alkyl, optionally substituted aryl or optionally substituted heteroaryl, provided that both Q and Y are not simultaneously absent;
Alk2 is absent or present and is selected from hydrogen, or an optionally substituted, straight chain or branched, alkyl, alkenyl or alkynyl group having from 1 to 30 carbon atoms;
R2 is selected from hydrogen, C1-4alkyl or R2 can combine with R1 and N to form an azide;
R3 and R4 are independently selected from hydrogen, carbamoyl and C1-4acyl;
R5 is selected from hydrogen, fucopyranosyl, carbamoyl and C1-4acyl;
R6 is selected from hydrogen, C1-4acyl or a monosaccharide;
R7 is independently selected from an acetamide or a hydroxyl group;
R8 is selected from hydrogen, sulphonato, C1-4acyl or a monosaccharide;
R9 is selected from hydrogen or a monosaccharide;
R10 is selected from hydrogen or optionally substituted C1-4alkyl;
R11 is selected from hydrogen, a monosaccharide, glycerol, C1-4acyl or C1-4alkyl;
R12 is selected from hydrogen, fucopyranosyl or C1-4acyl;
R13 is independently selected from hydrogen or fucopyranosyl;
m is an integer selected from 0 and 1;
n is an integer selected from 0 to 3; and
where the reducing end sugar ring is in open chain or ring closed form.
3. The method according to claim 2 wherein R1 is hydrogen.
4. The method according to claim 2 wherein R1 is \u2014X-Alk and wherein Alk is selected from an optionally substituted, straight chain or branched, alkyl, alkenyl or alkynyl group having from 5 to 25 carbon atoms.
5. The method according to claim 4 wherein Alk is selected from an optionally substituted, straight chain or branched, alkyl, alkenyl or alkynyl group having from 10 to 25 carbon atoms.
6. The method according to claim 5 wherein Alk is selected from an optionally substituted, straight chain or branched, alkyl, alkenyl or alkynyl group having from 14 to 22 carbon atoms.
7. The method according to claim 4 wherein X is \u2014C(O)\u2014.
8. The method according to claim 2 wherein m is 1 and n is an integer selected from 1 to 2.
9. The method according to claim 2 wherein R1 is \u2014X-Alk1-Q-Y-Alk2 and wherein X is \u2014C(O)\u2014, Alk1 is selected from divalent C1-4alkyl or is absent, Q is selected from optionally substituted divalent aryl or optionally substituted divalent heteroaryl, Y is selected from \u2014O\u2014, \u2014NH\u2014, \u2014S\u2014, \u2014NHC(O)\u2014, or \u2014C(O)NH\u2014, and Alk2 is an optionally substituted C1-25alkyl or C1-24alkenyl group.
10-11. (canceled)
12. The method according to claim 2 wherein R2 is hydrogen.
13. The method according to claim 2 wherein R3, R4 and R5 are independently selected from hydrogen, carbamoyl or acetyl.
14-15. (canceled)
16. The method according to claim 2 wherein R6 is hydrogen.
17. The method according to claim 2 wherein R7 is an acetamide.
18. The method according to claim 2 wherein R8 is selected from hydrogen, sulphonato, C1-4acyl, an unsubstituted monosaccharide, or a substituted monosaccharide of formula III:
wherein:
Rx is selected from hydrogen, C1-4alkyl or C1-4acyl;
Ry is selected from hydrogen, sulphonato or C1-4acyl;
Rz is selected from hydrogen, C1-4alkyl or C1-4acyl; and
RH is selected from H or ORP, wherein RP is selected from hydrogen, C1-4alkyl or C1-4acyl.
19. The method according claim 2 wherein R8 is selected from hydrogen, arabinosyl, sulphonato, C1-4acyl or a substituted monosaccharide of formula IV:
wherein:
Rx is selected from hydrogen, C1-4alkyl or C1-4acyl;
Ry is selected from hydrogen, sulphonato or C1-4acyl; and
Rz is selected from hydrogen, C1-4alkyl or C1-4acyl.
20. The method according to claim 19 wherein R8 is selected from sulphonato or a substituted monosaccharide of formula IV wherein Rz is selected from acetyl or hydrogen, R is hydrogen, and R is selected from hydrogen or methyl.
21-29. (canceled)
30. The method according to claim 2 wherein R2 is hydrogen or C1-4alkyl; R3, R4 and R5 are independently selected from hydrogen, carbamoyl and C1-4acyl; R6 is hydrogen, C1-4acyl or \u03b1-L-fucopyranosyl; each R7 is independently selected from an acetamide or a hydroxyl group; R8 is hydrogen, arabinosyl, sulphonato, C1-4acyl or a substituted monosaccharide of formula IV:
wherein:
Rx is hydrogen or C1-4acyl, Ry is hydrogen, sulphonato or C1-4acyl, and Rz is hydrogen, C1-4alkyl or C1-4acyl;
R9 is hydrogen, \u03b1-L-fucosyl or arabinosyl;
R10 is hydrogen, methyl or substituted methyl;
R11 is hydrogen, mannosyl, glycerol or C1-4alkyl;
R12 is hydrogen or C1-4acyl;
m is the integer 1; and
n is an integer selected from 1 or 2.
31. The method according to claim 2 wherein the oligosaccharide is of formula V
wherein:
R1 is selected from hydrogen, \u2014X-Alk or \u2014X-Alk1-Q-Y-Alk2;
R2 is selected from hydrogen or methyl;
R3 and R4 are independently selected from hydrogen and carbamoyl;
Rz is selected from hydrogen or acetyl;
Rx is selected from hydrogen or methyl; and n is an integer selected from 1 or 2.
32-34. (canceled)
35. The method according to claim 2 wherein the oligosaccharide is of formula VI:
wherein:
R1 is selected from hydrogen, \u2014X-Alk or \u2014X-Alk1-Q-Y-Alk2; and n is an integer selected from 1 or 2.
36. The method according to claim 2 wherein the oligosaccharide is of formula VII:
wherein:
R1 is selected from hydrogen, \u2014X-Alk or \u2014X-Alk1-Q-Y-Alk2; and
n is an integer selected from 1 or 2.
37. The method according to claim 2 wherein the oligosaccharide is of formula VIII:
wherein:
R1 is \u2014X-Alk1-Q-Y-Alk2;
X is selected from \u2014C(O)\u2014, \u2014C(NRN)\u2014, \u2014C(S)\u2014, \u2014SO2\u2014, \u2014P(O)(ORN)\u2014 wherein RN is hydrogen, hydroxy, amino, optionally substituted C1-8alkyl, optionally substituted C2-8alkenyl, optionally substituted C2-8alkynyl, optionally substituted C1-4alkylaryl, and optionally substituted aryl;
Alk1 is absent or present and is selected from an optionally substituted divalent C1-10alkyl, optionally substituted divalent C2-10alkenyl and optionally substituted divalent C2-10alkynyl chain;
Q is absent or present and is selected from an optionally substituted divalent cycloalkyl, optionally substituted divalent cycloalkenyl, optionally substituted divalent heterocycle, optionally substituted divalent aryl or optionally substituted divalent heteroaryl ring system;
Y is absent or present and is selected from \u2014NH\u2014, \u2014O\u2014, \u2014S\u2014, \u2014NHC(O)\u2014, \u2014C(O)NH\u2014, NHSO3\u2014, \u2014C(RG)\u2550N\u2014N\u2014, \u2014NHC(O)NH\u2014, \u2014NHC(S)NH\u2014, \u2014NHC(NH)NH\u2014, \u2014C(RG)\u2550N\u2014, and \u2014N\u2550C(R)\u2014, wherein RG is hydrogen, optionally substituted C1-6alkyl, optionally substituted arylC1-4alkyl, optionally substituted aryl or optionally substituted heteroaryl;
Alk2 is absent or present and is selected from an optionally substituted, straight chain or branched, alkyl, alkenyl or alkynyl group having from 1 to 30 carbon atoms; and
n is an integer selected from 1 or 2.
38-39. (canceled)
40. The method according to claim 1 wherein the Nod factor is neutral, or does not have a charge, positive or negative, of greater magnitude than 1.
41. A method of preventing or treating an angiogenesis associated disorder comprising administering to a subject in a need of such treatment a therapeutically effective amount of a Nod-factor as defined in claim 1.
42. (canceled)
43. The method according to claim 41 wherein said therapeutically effective amount is an amount effective to inhibit primary tumor formation and metastasis in solid tumors, said tumors being associated with a cancer selected from rhabdomyosarcomas, retinoblastoma, Ewing sarcoma, neuroblastoma, osteosarcoma, colon, prostate, head and neck, breast, bladder, liver, pancreatic lung, CNS, Paget’s disease and blood-born tumors such as leukemia and hemangiona.
44. The method according to claim 43 wherein the Nod factor is combined with at least one additional anti-cancer, anti-metastatic or antineoplastic agent.
45-64. (canceled)
65. An angiogenesis modulating composition comprising a Nod-factor as defined in claim 1.
66. An angiogenesis modulating composition comprising an oligosaccharide or salt of formula I as defined in claim 2.
67. The method of claim 2 wherein the oligosaccharide or salt is neutral, or does not have a charge, positive or negative, of greater magnitude than 1.
68. A method of preventing or treating an angiogenesis associated disorder comprising administering to a subject in a need of such treatment a therapeutically effective amount of an oligosaccharide or salt of formula I as defined in claim 2.
69. The method according to claim 68 wherein said therapeutically effective amount is an amount effective to inhibit primary tumor formation and metastasis in solid tumors, said tumors being associated with a cancer selected from rhabdomyosarcomas, retinoblastoma, Ewing sarcoma, neuroblastoma, osteosarcoma, colon, prostate, head and neck, breast, bladder, liver, pancreatic, lung, CNS, Paget’s disease and blood-born tumors such as leukemia and hemangiona.
70. The method according to claim 69 wherein the oligosaccharide or salt is combined with at least one additional anti-cancer, anti-metastatic or antineoplastic agent.