1. An information processing apparatus with a processor, the information processing apparatus comprising:
an instruction unit configured to instruct, using the processor, changing of layout for document data including a first page on which objects are arranged and a second page following the first page;
a display control unit configured to display, using the processor, a definition screen to define a page range;
a definition unit configured to define, using the processor, a page number as the page range of changing target in accordance with instruction input via the definition screen;
a reducing unit configured to reduce a distance between the objects arranged on a first page included in the page range to a predetermined distance shorter than the distance between the objects arranged on the first page when the changing of layout is instructed;
an arranging unit configured to arrange an object arranged on the second page in a space of the first page generated by reducing the distance between the objects arranged on the first page to the predetermined distance;
a determining unit configured to determine whether a ratio of the object arranged on the second page exceeds a threshold value; and
an eliminating unit configured to eliminate the second page by arranging the object arranged on the second page in the space of the first page,
wherein the reducing unit is configured to reduce the distance between the objects arranged on the first page included in the page range to the predetermined distance when the determining unit determines that the ratio does not exceed the threshold value.
2. The information processing apparatus according to claim 1, further comprising:
a setting unit configured to set a priority for a gap between the objects, wherein the reducing unit is configured to reduce the distance of the gap between the objects in a priority order from low to high based on the priority set by the setting unit.
3. An information processing method comprising:
instructing changing of layout for document data including a first page on which objects are arranged and a second page following the first page;
displaying, using a processor, a definition screen to define a page range;
defining, using the processor, a page number as the page range of changing target in accordance with instruction input via the definition screen;
reducing a distance between the objects arranged on a first page included in the page range to a predetermined distance shorter than the distance between the objects arranged on the first page when the changing of layout is instructed;
arranging an object arranged on the second page in a space of the first page generated by reducing the distance between the objects arranged on the first page to the predetermined distance;
determining whether a ratio of the object arranged on the second page exceeds a threshold value; and
eliminating the second page by arranging the object arranged on the second page in the space of the first page,
wherein the reducing unit is configured to reduce the distance between the objects arranged on the first page included in the page range to the predetermined distance when the determining unit determines that the ratio does not exceed the threshold value.
4. The information processing method according to claim 3, further comprising:
setting a priority for a gap between the objects, wherein reducing a distance of the gap between the objects arranged on a first page comprises reducing the distance between the objects in a priority order from low to high based on the priority set.
5. A computer readable device storing a program for causing an information processing apparatus to perform an information processing method comprising:
instructing changing of layout for document data including a first page on which objects are arranged and a second page following the first page;
displaying a definition screen to define a page range;
defining a page number as the page range of changing target in accordance with instruction input via the definition screen;
reducing a distance between the objects arranged on a first page included in the page range to a predetermined distance shorter than the distance between the objects arranged on the first page when the changing of layout is instructed;
arranging an object arranged on the second page in a space of the first page generated by reducing the distance between the objects arranged on the first page to the predetermined distance;
determining whether a ratio of the object arranged on the second page exceeds a threshold value; and
eliminating the second page by arranging the object arranged on the second page in the space of the first page,
wherein the reducing unit is configured to reduce the distance between the objects arranged on the first page included in the page range to the predetermined distance when the determining unit determines that the ratio does not exceed the threshold value.
6. The computer readable device according to claim 5, wherein the method further comprises:
setting a priority for a gap between the objects, wherein reducing a distance of the gap between the objects arranged on a first page comprises reducing the distance between the objects in a priority order from low to high based on the priority set.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for detecting the presence of cervical dysplasia in a subject, the method comprising
a) contacting a cervical sample from a subject with a reagent or reagents for detecting a level of at least one target RNA, wherein the at least one target RNA:
(i) is capable of specifically hybridizing to a nucleic acid having a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(ii) comprises a sequence that is complementary to at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(iii) comprises at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 345 to 388;
b) comparing the level of the at least one target RNA to a normal level of the RNA; and
c) detecting the presence of cervical dysplasia in the subject when the level of the at least one target RNA in the sample is greater than a normal level of the at least one target RNA.
2. (canceled)
3. A method for facilitating the detection of cervical dysplasia in a subject, comprising:
(a) contacting a cervical sample from a subject with a reagent or reagents for detecting a level of at least one target RNA, wherein the at least one target RNA:
(i) is capable of specifically hybridizing to a nucleic acid having a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(ii) comprises a sequence that is complementary to at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(iii) comprises at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 345 to 388; and
(b) comparing the level of the at least one target RNA to a normal level of the RNA; and
(c) communicating the results of (b) to a medical practitioner for the purpose of determining whether the subject has cervical dysplasia.
4. The method of claim 1, wherein detecting a level of at least one target RNA in a sample comprises:
(a) hybridizing nucleic acids of the sample with at least one polynucleotide that is complementary to a target RNA in the sample or to a complement thereof; and
(b) detecting at least one complex comprising a polynucleotide hybridized to at least one nucleic acid selected from the target RNA, a DNA amplicon of the target RNA, and a complement of the target RNA.
5. (canceled)
6. The method of claim 1, wherein the method further comprises isolating nucleic acids from the cervical sample.
7. The method of claim 6, wherein the nucleic acids comprise RNA that has been separated from DNA.
8. The method of claim 1, wherein at least one target RNA in its mature form comprises fewer than 30 nucleotides.
9. The method of claim 1, wherein at least one target RNA is a microRNA.
10. The method of claim 1, wherein levels of at least two target RNAs are detected, wherein at least two of the target RNAs:
(i) are capable of specifically hybridizing to a nucleic acid having a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(ii) comprise a sequence that is complementary to at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(iii) comprise at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 345 to 388; and
wherein the at least two target RNAs are different.
11-12. (canceled)
13. The method of claim 10, wherein levels of at least three target RNAs are detected, wherein at least three of the target RNAs:
(i) are capable of specifically hybridizing to a nucleic acid having a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(ii) comprise a sequence that is complementary to at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 1 to 41 and 133 to 211; or
(iii) comprise at least 15 contiguous nucleotides of a sequence selected from SEQ ID NOs: 345 to 388; and
wherein the at least three target RNAs are different.
14-16. (canceled)
17. The method of claim 10, wherein levels of at least five target RNAs are detected.
18. (canceled)
19. The method of claim 1, wherein the method further comprises detection of a level of at least one target RNA that is an mRNA.
20. The method of claim 19, wherein the mRNA is selected from CDKN2A, MKI67, TOP2A, MCM5, BIRC5, MMP9, and MCM2.
21.-24. (canceled)
25. A synthetic polynucleotide comprising a first region, wherein the first region comprises a sequence of at least 8 contiguous nucleotides that is identical or complementary to a sequence of at least 8 contiguous nucleotides of one of SEQ ID NOs: 1 to 7, 9 to 37, 133 to 201, and 345 to 388.
26-27. (canceled)
28. The synthetic polynucleotide of claim 25, wherein the first region comprises a sequence of at least 12 contiguous nucleotides that is identical or complementary to a sequence of at least 12 contiguous nucleotides of one of SEQ ID NOs: 1 to 7, 9 to 37, 133 to 201, and 345 to 388.
29. The synthetic polynucleotide of claim 25, wherein the polynucleotide comprises a detectable label.
30. The synthetic polynucleotide of claim 29, wherein the detectable label is a FRET label.
31. (canceled)
32. The synthetic polynucleotide of claim 25, wherein the polynucleotide further comprises a second region that is not identical or complementary to a region of the target RNA.
33-36. (canceled)
37. A kit comprising a synthetic polynucleotide of claim 25.
38. (canceled)
39. The kit of claim 37, wherein the kit further comprises at least one polymerase.
40. The kit of claim 37, wherein the kit further comprises dNTPs.
41. The method of claim 1, wherein the reagent is selected from a probe that specifically hybridizes to the RNA, a primer or primers for reverse transcribing the RNA into cDNA, a primer or primers for amplifying the cDNA, a microarray comprising at least one nucleic acid that hybridizes to the RNA or cDNA reverse transcribed from the RNA, and a primer for sequencing the RNA or cDNA reverse transcribed from the RNA.