1. A manufacturing method of a three dimensional structure having a hydrophobic inner surface, comprising:
anodizing a three dimensional metal member and forming fine holes on an external surface of the metal member;
forming a replica by coating a non-wetting polymer material on the outer surface of the metal member and forming the non-wetting polymer material to be a replication structure corresponding to the fine holes of the metal member;
forming an exterior by surrounding the replication structure with an exterior forming material; and
etching the metal member and eliminating the metal member from the replication structure and the exterior forming material.
2. The manufacturing method of claim 1, wherein the exterior forming material has adhesion on its surface contacting the replication structure.
3. The manufacturing method of claim 1, wherein the exterior forming material has flexibility so as to be adhered on a curved external surface of the replication structure.
4. The manufacturing method of claim 2, wherein the exterior forming material is an acryl film.
5. The manufacturing method of claim 1, further comprising, before anodizing, spraying fine particles and forming fine protrusions and depressions on the external surface of the metal member.
6. The manufacturing method of claim 5, wherein the metal member is formed in a cylindrical shape, and the fine particles are sprayed on a circumferential surface of the metal member.
7. The manufacturing method of claim 6, wherein the exterior forming material is adhered on an area corresponding to the circumferential surface of the metal member.
8. The manufacturing method of claim 1, wherein the non-wetting polymer material is provided in the fine holes of the metal member, and the replication structure has a plurality of columns corresponding to the fine holes.
9. The manufacturing method of claim 8, wherein the plurality of columns partially stick to each other to form a plurality of groups.
10. The manufacturing method of claim 1, wherein the metal member is wet-etched.
11. The manufacturing method of claim 1, wherein the metal member is formed of an aluminum material.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A complex of enzyme, protein and carrier comprising
(1) a carrier;
(2) an enzyme, two or more molecules of the enzyme being conjugated to the carrier in (1); and
(3) a protein with a specific binding potency to other substance(s), the protein being conjugated to at least one molecule of the two or more molecules of the enzyme in (2).
2. A complex of enzyme, protein and carrier comprising
(1) a carrier;
(2) an enzyme, two or more molecules of the enzyme being conjugated to the carrier in (1);
(3) a protein with a specific binding potency to other substances, the protein being conjugated to at least one molecule of the two or more molecules of the enzyme in (2); and
(4) the same protein as in (3), conjugated directly to the carrier in (1).
3. The complex of enzyme, protein and carrier according to claim 1, wherein the carrier has an average molecular weight of 5,000 to 500,000 Da, determined by gel filtration chromatography.
4. The complex of enzyme, protein and carrier according to claim 2, wherein the carrier has an average molecular weight of 5,000 to 500,000 Da, determined by gel filtration chromatography.
5. The complex of enzyme, protein and carrier according to claim 3, wherein the carrier has an average molecular weight of 10,000 to 300,000 Da, determined by gel filtration chromatography.
6. The complex of enzyme, protein and carrier according to claim 4, wherein the carrier has an average molecular weight of 10,000 to 300,000 Da, determined by gel filtration chromatography.
7. The complex of enzyme, protein and carrier according to claim 1, wherein the carrier has two or more amino groups.
8. The complex of enzyme, protein and carrier according to claim 2, wherein the carrier has two or more amino groups.
9. The complex of enzyme, protein and carrier according to claim 1, wherein the carrier is one selected from the group consisting of (a) a peptide polymer having two or more basic amino groups and (b) a polysaccharide having introduced active groups, the kind of the active groups being at least one kind selected from the group consisting of amino group, aldehyde group and vinyl group.
10. The complex of enzyme, protein and carrier according to claim 2, wherein the carrier is one selected from the group consisting of (a) a peptide polymer having two or more basic amino groups and (b) a polysaccharide having introduced active groups, the kind of the active groups being at least one kind selected from the group consisting of amino group, aldehyde group and vinyl group.
11. The complex of enzyme, protein and carrier according to claim 9, wherein the carrier is polylysine.
12. The complex of enzyme, protein and carrier according to claim 10, wherein the carrier is polylysine.
13. The complex of enzyme, protein and carrier according to claim 1, wherein the enzyme is one selected from the group consisting of horse radish peroxidase, alkaline phosphatase, -galactosidase and glucose oxidase.
14. The complex of enzyme, protein and carrier according to claim 2, wherein the enzyme is one selected from the group consisting of horse radish peroxidase, alkaline phosphatase, -galactosidase and glucose oxidase.
15. The complex of enzyme, protein and carrier according to claim 1, wherein the protein with a specific binding potency to other substance(s) is at least one selected from the group consisting of an antibody and fragment(s) thereof.
16. The complex of enzyme, protein and carrier according to claim 2, wherein the protein with a specific binding potency to other substance(s) is at least one selected from the group consisting of an antibody and fragment(s) thereof.
17. The complex of enzyme, protein and carrier according to claim 15, wherein the antibody is a polyclonal antibody or monoclonal antibody.
18. The complex of enzyme, protein and carrier according to claim 16, wherein the antibody is a polyclonal antibody or monoclonal antibody.
19. The complex of enzyme, protein and carrier according to claim 15, wherein the fragment(s) means at least one selected from the group consisting of F(ab)2, Fab and Fabc.
20. The complex of enzyme, protein and carrier according to claim 16, wherein the fragment(s) means at least one selected from the group consisting of F(ab)2, Fab and Fabc.
21. The complex of enzyme, protein and carrier according to claim 1, wherein the protein with a specific binding potency to other substance(s) is avidin or streptavidin.
22. The complex of enzyme, protein and carrier according to claim 2, wherein the protein with a specific binding potency to other substance(s) is avidin or streptavidin.
23. An immunoassay kit comprising the complex according to claim 1.
24. An immunoassay kit comprising the complex according to claim 2.
25. The immunoassay kit according to claim 23, wherein the immunoassay is immunohistostaining or enzyme immunoassay.
26. The immunoassay kit according to claim 24, wherein the immunoassay is immunohistostaining or enzyme immunoassay.