What is claimed is:
1. A method for purification of a proteinaceous material from a mixture of the proteinaceous material and interferants comprising:
a) supplying a first end of a hollow fiber filter with a mixture of a proteinaceous material having a molecular weight between approximately 50,000 and 1,000,000 and interferants having a molecular weight that is less than 50% of the molecular weight of the proteinaceous material to a first end of a hollow fiber filter;
b) applying a pressure force to a lumen of the hollow fiber filter to cause the interferants in the mixture to pass through the membrane of the hollow fiber filter;
c) adding buffer or other fluid which does not react with the proteinaceous material to further cause the interferants to pass through the membrane of the hollow fiber filter; and
d) recovering the proteinaceous material from a second end of the hollow fiber filter, said second end being disposed at an opposite end of the hollow fiber filter from the first end.
2. The method of claim 1, wherein said proteinaceous material is selected from the group consisting of antibodies, activated antibodies, fluorescent labels, activated fluorescent labels, and conjugated antibody fluorescent label.
3. The method of claim 1, wherein said interferants have a molecular weight that is less than 10% of the molecular weight of the proteinaceous material.
4. The method of claim 1, wherein said pressure force used to cause the interferants in the mixture to pass through the membrane of the hollow fiber filter is a positive pressure force.
5. The method of claim 1, wherein the hollow fiber filter has a molecular weight cut off of from about 1,000 to 50,000.
6. The method of claim 1, wherein the proteinaceous material recovered from the second end of the hollow fiber filter is at a concentration greater than 40%.
7. The method of claim 1, wherein the proteinaceous material recovered from the second end of the hollow fiber filter is at a concentration greater than 60%.
8. A method for purification of a biological macromolecule from a mixture of the biological macromolecule and interferants comprising:
a) supplying a first end of a hollow fiber filter with a mixture of a biological macromolecule having a molecular weight between approximately 20,000 and 2,000,000 and interferants having a molecular weight that is less than 50% of the molecular weight of the biological macromolecule to a first end of a hollow fiber filter;
b) applying a pressure force to a lumen of the hollow fiber filter to cause the interferants in the mixture to pass through the membrane of the hollow fiber filter;
c) adding buffer or other fluid which does not react with the biological macromolecule to further cause the interferants to pass through the membrane of the hollow fiber filter; and
d) recovering the biological macromolecule from a second end of the hollow fiber filter, said second end being disposed at an opposite end of the hollow fiber filter from the first end.
9. The method of claim 1, wherein said biological macromolecule is selected from the group consisting of nucleic acids and complex carbohydrates.
10. The method of claim 1, wherein said interferants have a molecular weight that is less than 10% of the molecular weight of the biological macromolecule.
11. The method of claim 1, wherein said pressure force used to cause the interferants in the mixture to pass through the membrane of the hollow fiber filter is a positive pressure force.
12. The method of claim 1, wherein the hollow fiber filter has a molecular weight cut off of from about 3,000 to 5,000.
13. The method of claim 1, wherein the biological macromolecule recovered from the second end of the hollow fiber filter is at a concentration greater than 20%.
14. The method of claim 1, wherein the proteinaceous material recovered from the second end of the hollow fiber filter is at a concentration greater than 30%.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A system comprising:
a magnetic emulator, wherein said magnetic emulator is operable to electrically couple, and communicate data to, a read-head located on a magnetic stripe reader; and
a non-moveable magnetic shielding located about said magnetic emulator, wherein said read-head is inoperable to receive data from said magnetic emulator when said magnetic shielding is between said magnetic emulator and said read-head.
2-3. (canceled)
4. The system of claim 1, further comprising:
a source of energy; and
a regulator for regulating said source of energy, wherein said regulated energy is utilized by said magnetic emulator.
5. The system of claim 1, wherein said communicated data includes a dynamic payment number and a dynamic code.
6. The card of claim 1, further comprising a processor.
7. The card of claim 1, further comprising a plurality of flexible layers of circuit board.
8. The card of claim 1, further comprising a flexible layer of circuit board.
9. The card of claim 1, further comprising a second magnetic emulator.
10. The card of claim 1, further comprising:
a processor; and
a button.
11. The card of claim 1, wherein said magnetic emulator is operable to receive data from an encoding head of said magnetic stripe reader.
12. The card of claim 1, further comprising a button.
13. The card of claim 1, further comprising a magnetic stripe encoding receiver operable to receive data from an encoding head of said magnetic stripe reader.
14. The card of claim 1, further comprising a battery.
15. The card of claim 1, further comprising a read-head detector for detecting said read-head.
16. The card of claim 1, further comprising an RFID field detector.
17. The card of claim 1, further comprising:
a read-head detector; and
an RFID field detector.
18. The card of claim 1, further comprising:
a battery; and
a button.
19. The card of claim 1, wherein said magnetic emulator includes a coil.
20. The card of claim 1, further comprising:
a battery; and
a processor.
21. The card of claim 1, further comprising:
a second magnetic shielding located about said magnetic emulator.