1. A method for identifying or quantifying a plurality of small molecule analytes, the method comprising:
providing a control sample comprising known quantities of a plurality of small molecule analytes, each analyte comprising a different reactive functional group;
contacting a first isotopic variant of a labeling reagent with each said analyte in the control sample to yield a plurality of first isotopically labeled analytes, wherein the labeling reagent is covalently linked to the analytes at the different reactive functional groups;
providing an experimental sample comprising a plurality of said small molecule analytes;
contacting a second isotopic variant of the labeling reagent with each said analyte in the experimental sample to yield a plurality of second isotopically labeled analytes, wherein the first and second isotopically labeled analytes are chemically equivalent yet isotopically distinct;
mixing at least portions of the control and experimental samples to yield a combined sample; and
subjecting the combined sample to mass spectrometric analysis to identify or quantify the plurality of analytes in the experimental sample.
2. A method for identifying or quantifying a plurality of small molecule analytes, the method comprising:
providing a control sample comprising known quantities of a plurality of small molecule analytes, each analyte comprising a different reactive functional group;
providing an experimental sample comprising a plurality of said small molecule analytes;
dividing the experimental sample into first and second experimental subsamples;
adding an amount of the control sample to the first experimental subsample;
contacting a first isotopic variant of a labeling reagent with each said analyte in the control sample to yield a plurality of first isotopically labeled analytes, wherein the labeling reagent is covalently linked to the analytes at the different reactive functional groups;
contacting a second isotopic variant of the labeling reagent with each said analyte in each of the first and second experimental subsamples to yield first and second experimental subsamples each comprising a plurality of second isotopically labeled analytes, wherein the first and second isotopically labeled analytes are chemically equivalent yet isotopically distinct;
adding equal amounts of the control sample to each of the first and second experimental subsamples to yield first and second combined samples;
subjecting each of the first and second combined samples to mass spectrometric analysis; and
comparing peak intensities generated by the first and second combined samples to identify or quantify the plurality of analytes in the experimental sample.
3. A method for detecting differences in the concentrations of a plurality of small molecule analytes present in first and second samples, the method comprising:
providing first and second samples, each sample comprising a plurality of small molecule analytes, each analyte comprising a different reactive functional group;
contacting a first isotopic variant of a labeling reagent with each said analyte in the first sample to yield a plurality of first isotopically labeled analytes, wherein the labeling reagent is covalently linked to the analytes at the different reactive functional groups;
contacting a second isotopic variant of the labeling reagent with each said analyte in the second sample to yield a plurality of second isotopically labeled analytes, wherein the first and second isotopically labeled analytes are chemically equivalent yet isotopically distinct;
mixing at least portions of the first and second samples to yield a combined sample; and
subjecting the combined sample to mass spectrometric analysis to determine a normalized isotope ratio characterizing analytes whose concentration is the same in the first and second samples and an isotope ratio of the first and second isotopically labeled analytes, wherein a difference in the isotope ratio of the first and second isotopically labeled analytes and the normalized isotope ratio is indicative of a difference in concentration of the analyte in the first and second samples.
4. A method for identifying a small molecule analyte in a sample, the method comprising:
providing a sample comprising a plurality of small molecule analytes, each analyte comprising a different reactive functional group;
dividing the sample into at least first and second portions;
contacting a first isotopic variant of a labeling reagent with each said analyte in the first portion to yield a plurality of first isotopically labeled analytes, wherein the labeling reagent is covalently linked to the analytes at the different reactive functional groups;
contacting a second isotopic variant of the labeling reagent with each said analyte in the second portion to yield a plurality of second isotopically labeled analytes, wherein the first and second isotopically labeled analytes are chemically equivalent yet isotopically distinct;
mixing the first and second portions to yield a combined sample; and
subjecting the combined sample to mass spectrometric analysis to yield mz values for at least one analyte; and
comparing the mz values to known or predicted mz values for a known analyte to identify the analyte.
5. The method of any of claims 1 to 4 further comprising subjecting the samples to fractionation prior to mass spectrometric analysis.
6. (canceled)
7. The method of any of claims 1 to 4 wherein the labeling reagent comprises an amine.
8. The method of claim 7 wherein the labeling reagent further comprises a hydrophobic moiety.
9. The method of claim 8 wherein the labeling reagent is aniline or an aniline derivative.
10. The method of any of claims 1 to 4 wherein the reactive functional groups comprise a functional group selected from the group consisting of a carbonyl, phosphate and carboxyl.
11. The method of claim 10 wherein the reactive function groups comprise carbonyl, phosphate and carboxyl.
12.-15. (canceled)
16. The method of any of claims 1 to 4 wherein the small molecule analytes comprise primary or secondary cellular metabolites.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for recording Internet Protocol (IP) media sessions through a network, comprising:
providing a recording system located externally to a communication system;
delivering to the recording system via telephony call integration (CTI) at least one characteristic of an IP media session;
assessing said at least one characteristic for compliance with at least one rule; and
if said at least one characteristic matches said at least one rule, sending a request from the recording system to a management unit of the communication system to forward data related to the IP media session to the recording system; and
forwarding data of the IP media session to the recording system after adjusting destination IP address and port.
2. The method of claim 1, wherein said at least one rule comprises a dynamic rule.
3. The method of claim 1, wherein said at least one rule comprises a static rule.
4. The method of claim 1, wherein said at least one rule comprises a deterministic rule.
5. The method of claim 1, wherein said at least one rule comprises a statistical rule.
6. The method of claim 5, wherein said statistical rule includes instructions to record a certain percentage of said session in a certain time interval.
7. The method of claim 1, wherein said at least one rule comprises an event driven rule.
8. The method of claim 1, wherein said at least one characteristic of the data comprises metadata.
9. The method of claim 1, comprising:
pre-processing the data of the IP media session before forwarding.
10. The method of claim 9, wherein said pre-processing comprises at least one of encapsulation, re-encapsulation, summation, compression, encryption or decryption of at least a portion of the data.
11. The method of claim 1 comprising compressing the data prior to forwarding.
12. The method of claim 1 comprising:
detecting initiation of the IP media session by a recording agent; and
contacting the recording device by the recording agent.
13. The method of claim 1, wherein said method further comprising at least one of the following steps:
displaying said forwarded data; and
recording said forwarded data.
14. The method of claim 1, wherein said at least one rule states that all data of the session is to be forwarded.
15. The method of claim 1, wherein said IP media session is one of the following sessions: voice over IP sessions, video conference sessions, chat sessions, a trunk radio sessions and web collaboration sessions.
16. The method of claim 1, wherein said step of forwarding comprises duplicating only a portion of the data.
17. The method of claim 1, wherein said at least one rule is driven by an event which occurs externally to the session.
18. The method of claim 1, wherein the forwarding is performed by one of the parties participating in the IP media session.
19. The method of claim 18, wherein one of the participating parties is an IP telephone.
20. The method of claim 18, wherein one of the participating parties is a gateway.