What is claimed is:
1. A method for the production of a polypeptide sensitive to proteolysis due to its content of arginine and lysine residues, wherein the method comprises the steps of:
(a) providing a yeast cell with reduced expression of either or both of the proteases encoded by YAP3 and MKC7;
(b) transforming the yeast cell of step (a) with a vector comprising a yeast promoter operably linked to a DNA sequence coding for the polypeptide sensitive to proteolysis;
(c) expressing the polypeptide; and
(d) isolating the polypeptide.
2. The method of claim 1 wherein the protease is encoded by YAP3.
3. The method of claim 1 wherein the protease is encoded by MKC7.
4. The method of claim 1 wherein the proteases are encoded by both YAP3 and MKC7.
5. The method of claim 1 wherein the reduced expression is due to an elimination of either or both of the proteases encoded by YAP3 and MKC7.
6. The method of claim 1 wherein the reduced expression is due to the disabling of either or both of the proteases encoded by YAP3 and MKC7.
7. The method of claim 1 wherein the reduced activity is due to antisense binding.
8. A method for the production of a polypeptide sensitive to proteolysis due to its content of arginine and lysine residues, wherein the method comprises the steps of:
(a) providing a yeast cell with reduced activity for either or both of the Yap3 and Mkc7 proteases;
(b) transforming the yeast cell of step (a) with a vector comprising a yeast promoter operably linked to a DNA sequence coding for the polypeptide sensitive to proteolysis;
(c) expressing the polypeptide; and
(d) isolating the polypeptide.
9. The method of claim 8 wherein the protease is the Yap3 protease.
10. The method of claim 8 wherein the protease is the Mkc7 protease.
11. The method of claim 8 wherein the proteases are both the Yap3 and Mkc7 proteases.
12. The method of claim 1 or claim 8 wherein the vector further comprises a signal peptide to direct secretion of the polypeptide from the yeast cell.
13. The method of claim 12 wherein the signal peptide is Saccharomyces cerevisiae -factor prepro sequence.
14. The method of claim 1 or claim 10 wherein the polypeptide further comprises a glycine residue at its carboxyl terminus.
15. The method of claim 14 further comprising treating the polypeptide with peptidyl-glycine amidating enzyme after isolating the polypeptide.
16. The method of claim 1 or 8 wherein the polypeptide is selected from the group consisting of a precursor of Manduca sexta diuretic hormone, a site-directed mutant M. sexta diuretic hormone, albumin, human parathyroid hormone, insulin, glicentine related polypeptide, glucagon, calcitonin, neuropeptide Y, and analogues of each of these polypeptides.
17. A method for the production of a polypeptide sensitive to proteolysis due to its content of arginine and lysine residues, wherein the method comprises the steps of:
(a) providing a eukaryotic cell with reduced expression of either or both of the proteases encoded by YAP3 and MKC7;
(b) transforming the eukaryotic cell of step (a) with a vector comprising a promoter operably linked to a DNA sequence coding for the polypeptide sensitive to proteolysis;
(c) expressing the polypeptide; and
(d) isolating the polypeptide.
18. A method for the production of a polypeptide sensitive to proteolysis due to its content of arginine and lysine residues, wherein the method comprises the steps of:
(a) providing a eukaryotic cell with reduced activity for either or both of the Yap3 and Mkc7 proteases;
(b) transforming the eukaryotic cell of step (a) with a vector comprising a promoter operably linked to a DNA sequence coding for the polypeptide sensitive to proteolysis;
(c) expressing the polypeptide; and
(d) isolating the polypeptide.
19. The method of claim 17 or claim 18 wherein the eukaryotic cell is selected from the group consisting of an insect cell, a plant cell and a mammalian cell.
20. The method of claim 1, claim 8, claim 16 or claim 17 wherein the polypeptide is a heterologous polypeptide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A circuit comprising:
an input adapted to receive a frame transmitted wireless from a station of a wireless local area network wherein the frame comprises a first basic service set identifier in a first field, a first source address in a second field and a first destination address in a third field;
wherein the circuit is configured to replace in the first field of the first frame the first basic service set identifier by a second basic service set identifier of an access point of the wireless local area network while maintaining the first source address and the first destination address; and
an output adapted to transfer the replaced first frame to a wireless transmission entity.
2. The circuit of claim 1,
wherein the circuit comprises a further input adapted to receive a second frame transmitted from the access point of the wireless local area network, the second frame comprising the second basic service set identifier in a first field and a second source address in a second field and a second destination address in a third field;
wherein the circuit is configured to replace in the first field of the second frame the second basic service set identifier by the first basic service set identifier while maintaining the second source address and the second destination address; and
a further output adapted to transfer the replaced second frame to the wireless transmission entity.
3. The circuit of claim 1, wherein the first frame further comprises a fourth field and fifth field, wherein the fourth field is set logic one for indicating that the first frame is transmitted to a distribution system, and the fifth field is set to logic zero for indicating that the second frame is not transmitted from a distribution system.
4. A WLAN system comprising:
a first access point;
a second access point, wherein the second access point is configured to receive from a first station a first frame comprising a basic service set identifier field, a source address field and a destination address field; and
wherein the second access point is configured to replace in the first frame the content of the basic service set identifier field by an identifier of the first access point while maintaining the content of the source address field and the destination address field; and wherein the second access point is configured to transmit the replaced first frame wireless to the first access point.
5. The WLAN system according to claim 4, wherein the second access point is configured to receive wireless from the first access point a second frame, the second frame comprising a destination address field, a basic set identifier field and a source address field;
wherein the second access point is configured to replace in the second frame the content of the basic service set identifier field by an identifier of the second access point while maintaining the content of the source address field and the destination address field; and
wherein the second access point is configured to transmit the replaced second frame to the first station.
6. The WLAN system according to claim 5, wherein the second access point conforms to IEEE 802.11.
7. A repeater device comprising:
an input adapted to receive a frame transmitted wireless from a station of a wireless local area network, the frame using three address fields; and
wherein the repeater device is configured to modify the frame and to wireless transfer the modified frame to a bridge, the modified frame using no other address fields than the three address fields.
8. The repeater device of claim 7, wherein a first one of the three address fields used in the frame records a basic service set identifier, a second one of the three address fields records a source address and a third one of the three address fields records a destination address.
9. The repeater device according to claim 8, wherein the repeater device is configured to modify the content of the first address field while maintaining the content of the second and third address field.
10. The repeater device according to claim 9, wherein the frame is a data frame conformable to IEEE 802.11.
11. A WLAN system comprising:
a bridge access point; and
a repeater access point, wherein the repeater access point is configured to receive from a station a frame and to wirelessly transmit the received frame to the bridge access point, wherein the WLAN system is configured to use only three address fields in the frame when transmitting the received frame from the repeater access point to the bridge access point.
12. The WLAN system according to claim 11, wherein a first one of the three address fields used in the frame records a basic service set identifier, a second one of the three address fields records a source address and a third one of the three address fields records a destination address.
13. The WLAN system according to claim 12, wherein the repeater access point is configured to modify the content of the first address field while maintaining the content of the second and third address field.
14. The WLAN system according to claim 11, wherein the frame is a data frame conformable to IEEE 802.11.